BC12,GDD1,YD1,LGW

2015-01-20 | Categories genes  | Tags ga  root  culm  dwarf  cell elongation  cell division  cellulose  leaf  leaf color  development  grain  grain length  grain size  nucleus  grain weight 

• Information
  • Symbol: BC12,GDD1,YD1,LGW
  • MSU: LOC_Os09g02650
  • RAPdb: Os09g0114500
• Publication
  • Brittle Culm 12, a dual-targeting kinesin-4 protein, controls cell-cycle progression and wall properties in rice, 2010, Plant J.
  • Mutation of rice BC12/GDD1, which encodes a kinesin-like protein that binds to a GA biosynthesis gene promoter, leads to dwarfism with impaired cell elongation, 2011, Plant Cell.
  • Characterization of a novel allele of bc12/gdd1 indicates a differential leaf color function for BC12/GDD1 in Indica and Japonica backgrounds, 2020, Plant Science.
  • Low grain weight, a new allele of BRITTLE CULM12, affects grain size through regulating GW7 expression in rice., 2022, Front Plant Sci.
• Genbank accession number
  • AK100974
• Key message
  • Therefore, BC12/GDD1, a kinesin-like protein with transcription regulation activity, mediates cell elongation by regulating the GA biosynthesis pathway in rice
  • Mutation of rice BC12/GDD1, which encodes a kinesin-like protein that binds to a GA biosynthesis gene promoter, leads to dwarfism with impaired cell elongation
  • Mitotic microtubule array comparison, flow cytometric analysis and expression assays of cyclin-dependent kinase (CDK) complexes in root-tip cells showed that cell-cycle progression is affected in bc12 mutants
  • GDD1 was cloned by a map-based approach, was expressed constitutively, and was found to encode the kinesin-like protein BRITTLE CULM12 (BC12)
  • bc12 mutants display dwarfism resulting from a significant reduction in cell number and brittleness due to an alteration in cellulose microfibril orientation and wall composition
  • Here, we report the functional characterization of Brittle Culm12 (BC12), a gene encoding a kinesin-4 protein
  • BC12 is expressed mainly in tissues undergoing cell division and secondary wall thickening
  • Therefore, BC12 functions as a dual-targeting kinesin protein and is implicated in cell-cycle progression, cellulose microfibril deposition and wall composition in the monocot plant rice
  • In this study we reveal that the novel BC12328Leu allele of BC12 modulated plant leaf color in yd1 plants, which has not been previously reported in studies of BC12/GDD1/MTD1/SRG1
  • We conclude that LGW regulates grain development by directly binding to the GW7 promoter and activating its expression
  • Overexpression of LGW increased the grain length, indicating that LGW is a positive regulator for regulating grain length
  • Elevating the GW7 expression levels in lgw plants rescued the small grain size phenotype
  • Our findings revealed that LGW plays an important role in regulating grain size, and manipulation of this gene provides a new strategy for regulating grain weight in rice
  • Protein sequence alignment analysis revealed that the mutation site was located at the nuclear localization signal (NLS) of LGW/BC12, resulting in the lgw protein not being located in the nucleus
• Connection
  • BC12~GDD1~YD1~LGW, GW7~GL7~SLG7~Chalk7, Low grain weight, a new allele of BRITTLE CULM12, affects grain size through regulating GW7 expression in rice., Elevating the GW7 expression levels in lgw plants rescued the small grain size phenotype
  • BC12~GDD1~YD1~LGW, GW7~GL7~SLG7~Chalk7, Low grain weight, a new allele of BRITTLE CULM12, affects grain size through regulating GW7 expression in rice., We conclude that LGW regulates grain development by directly binding to the GW7 promoter and activating its expression

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