PTC1,OsMS1

2015-01-20 | Categories genes  | Tags anther  sterility  cell death  tapetal  pollen  microspore  anther development  tapetum  reproductive  development  pollen wall  PCD  transcriptional activator  pollen exine formation  tapetal programmed cell death  transcription factor  temperature  cytoplasm  male sterility 

• Information
  • Symbol: PTC1,OsMS1
  • MSU: LOC_Os09g27620
  • RAPdb: Os09g0449000
• Publication
  • PERSISTENT TAPETAL CELL1 encodes a PHD-finger protein that is required for tapetal cell death and pollen development in rice, 2011, Plant Physiol.
  • OsMS1 functions as a transcriptional activator to regulate programmed tapetum development and pollen exine formation in rice., 2019, Plant Mol Biol.
  • OsMS188 Is a Key Regulator of Tapetum Development and Sporopollenin Synthesis in Rice, 2021, Rice (N Y).
  • A natural allele of OsMS1 responds to temperature changes and confers thermosensitive genic male sterility., 2022, Nat Commun.
• Genbank accession number
  • AK288120
• Key message
  • PTC1 encodes a PHD-finger (for plant homeodomain) protein, which is expressed specifically in tapetal cells and microspores during anther development in stages 8 and 9, when the wild-type tapetal cells initiate a typical apoptosis-like cell death
  • Microarray analysis indicated that 2,417 tapetum- and microspore-expressed genes, which are principally associated with tapetal development, degeneration, and pollen wall formation, had changed expression in ptc1 anthers
  • Moreover, the regulatory role of PTC1 in anther development was revealed by comparison with MS1 and other rice anther developmental regulators
  • These findings suggest a diversified and conserved switch of PTC1/MS1 in regulating programmed male reproductive development in both dicots and monocots, which provides new insights in plant anther development
  • The evolutionary significance of PTC1 was revealed by partial genetic complementation of the homologous mutation MALE STERILITY1 (MS1) in the dicot Arabidopsis (Arabidopsis thaliana)
  • Here, we report a key regulator in monocot rice (Oryza sativa), PERSISTANT TAPETAL CELL1 (PTC1), which controls programmed tapetal development and functional pollen formation
  • Even though ptc1 mutants show phenotypic similarity to ms1 in a lack of tapetal DNA fragmentation, delayed tapetal degeneration, as well as abnormal pollen wall formation and aborted microspore development, the ptc1 mutant displays a previously unreported phenotype of uncontrolled tapetal proliferation and subsequent commencement of necrosis-like tapetal death
  • OsMS1 functions as a transcriptional activator to regulate programmed tapetum development and pollen exine formation in rice.
  • OsMS1 functions as a transcriptional activator and interacts with known tapetal regulatory factors through its plant homeodomain (PHD) regulating tapetal programmed cell death (PCD) and pollen exine formation in rice
  • In addition, cytological observation revealed delayed tapetal PCD, defective pollen exine formation and a lack of DNA fragmentation according to a TUNEL analysis in the anthers of osms1 mutant
  • Results of qPCR suggested that the expression of the genes associated with tapetal PCD and pollen wall biosynthesis, such as EAT1, AP37, AP25, OsC6 and OsC4, were significantly reduced in osms1 mutant
  • Taken together, our results demonstrate that the interaction of OsMS1 with known tapetal regulatory factors through its PHD finger regulates tapetal PCD and pollen exine formation in rice
  • OsMS1 associates with the transcription factor TDR to regulate expression of downstream genes in a temperature-dependent manner
  • A natural allele of OsMS1 responds to temperature changes and confers thermosensitive genic male sterility.
  • Temperature regulates the abundances of OsMS1 and OsMS1(wenmin1) proteins
  • The high temperature causes more reduction of OsMS1(wenmin1) than OsMS1 in nuclei
  • Here, we show that OsMS1 is a histone binding protein, and its natural allele OsMS1(wenmin1) confers thermosensitive male sterility in rice
  • OsMS1 is primarily localized in nuclei, while OsMS1(wenmin1) is localized in nuclei and cytoplasm
• Connection
  • OsTDF1, PTC1~OsMS1, The functional analysis of OsTDF1 reveals a conserved genetic pathway for tapetal development between rice and Arabidopsis, The qRT-PCR analysis revealed that OsTDF1 acts downstream of UDT1 and upstream of TDR, EAT1, OsMYB103 and PTC1 in rice, suggesting that the genetic pathway for tapetum development is generally conserved between rice and Arabidopsis
  • AP37~OsERF3, PTC1~OsMS1, OsMS1 functions as a transcriptional activator to regulate programmed tapetum development and pollen exine formation in rice., Results of qPCR suggested that the expression of the genes associated with tapetal PCD and pollen wall biosynthesis, such as EAT1, AP37, AP25, OsC6 and OsC4, were significantly reduced in osms1 mutant
  • DTD~EAT1, PTC1~OsMS1, OsMS1 functions as a transcriptional activator to regulate programmed tapetum development and pollen exine formation in rice., Results of qPCR suggested that the expression of the genes associated with tapetal PCD and pollen wall biosynthesis, such as EAT1, AP37, AP25, OsC6 and OsC4, were significantly reduced in osms1 mutant
  • OSC4, PTC1~OsMS1, OsMS1 functions as a transcriptional activator to regulate programmed tapetum development and pollen exine formation in rice., Results of qPCR suggested that the expression of the genes associated with tapetal PCD and pollen wall biosynthesis, such as EAT1, AP37, AP25, OsC6 and OsC4, were significantly reduced in osms1 mutant
  • OsC6, PTC1~OsMS1, OsMS1 functions as a transcriptional activator to regulate programmed tapetum development and pollen exine formation in rice., Results of qPCR suggested that the expression of the genes associated with tapetal PCD and pollen wall biosynthesis, such as EAT1, AP37, AP25, OsC6 and OsC4, were significantly reduced in osms1 mutant
  • OsMADS15~DEP, PTC1~OsMS1, OsMS1 functions as a transcriptional activator to regulate programmed tapetum development and pollen exine formation in rice., Y2H and BiFC assays demonstrated that OsMS1 can interact with OsMADS15 and TDR INTERACTING PROTEIN2 (TIP2)
  • PTC1~OsMS1, TDR, OsMS1 functions as a transcriptional activator to regulate programmed tapetum development and pollen exine formation in rice., Y2H and BiFC assays demonstrated that OsMS1 can interact with OsMADS15 and TDR INTERACTING PROTEIN2 (TIP2)
  • PTC1~OsMS1, TIP2~bHLH142, OsMS1 functions as a transcriptional activator to regulate programmed tapetum development and pollen exine formation in rice., Y2H and BiFC assays demonstrated that OsMS1 can interact with OsMADS15 and TDR INTERACTING PROTEIN2 (TIP2)
  • PTC1~OsMS1, PTC2, PERSISTENT TAPETAL CELL 2 is required for normal tapetal programmed cell death and pollen wall patterning., The defective tapetal PCD phenotype of ptc2 was similar to that of a PCD delayed mutant, ptc1, in rice, while the abnormal pollen wall patterning resembled that of a pollen wall defective mutant, tek, in Arabidopsis thaliana
  • PTC1~OsMS1, RMS2~OsGELP34, RMS2 encoding a GDSL lipase mediates lipid homeostasis in anthers to determine rice male fertility., Furthermore, RMS2 is directly targeted by male fertility regulators Undeveloped Tapetum 1 (UDT1) and Persistent Tapetal Cell 1 (PTC1) both in vitro and in vivo, suggesting that RMS2 may serve as a key node in the rice male fertility regulatory network
  • PTC1~OsMS1, TDR, A natural allele of OsMS1 responds to temperature changes and confers thermosensitive genic male sterility., OsMS1 associates with the transcription factor TDR to regulate expression of downstream genes in a temperature-dependent manner

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