ISA3,OsISA3

• Symbol: ISA3,OsISA3
• MSU: LOC_Os09g29404
• RAPdb: Os09g0469400

• Rice debranching enzyme isoamylase3 facilitates starch metabolism and affects plastid morphogenesis, 2011, Plant Cell Physiol.
• Co-Overexpression of Two Key Source Genes, OsBMY4 and OsISA3, Improves Multiple Key Traits of Rice Seeds., 2022, J Agric Food Chem.

Genbank accession number

• Furthermore, chloroplasts in the leaf blade of isa3 seedlings were large and pleomorphic
• We investigated whether ISA3 plays a role in amyloplast development and starch metabolism in the developing endosperm
• ISA3-green fluorescent protein (GFP) fusion protein expressed under the control of the rice ISA3 promoter was targeted to the amyloplast stroma in the endosperm
• Both overexpression and loss of function of ISA3 in the endosperm generated pleomorphic amyloplasts and starch granules
• A transposon-inserted rice mutant of isoamylase3 (isa3) contained an increased amount of starch in the leaf blade at the end of the night, indicating that ISA3 plays a role in the degradation of transitory starch during the night
• Overexpression of ISA3 in the sugary1 mutant, which is deficient in ISA1 activity, did not convert water-soluble phytoglycogen to starch granules, indicating that ISA1 and ISA3 are not functionally redundant
• These results suggest that ISA3 facilitates starch metabolism and affects morphological characteristics of plastids in rice
• Systematic analyses of the transgenic rice indicated that co-overexpression of OsBMY4 and OsISA3 not only promoted rice yield and quality, but also improved seed germination and stress tolerance

• ISA3~OsISA3, OsBMY4, Co-Overexpression of Two Key Source Genes, OsBMY4 and OsISA3, Improves Multiple Key Traits of Rice Seeds, Co-Overexpression of Two Key Source Genes, OsBMY4 and OsISA3, Improves Multiple Key Traits of Rice Seeds