OsTKPR1,OsDFR2,OS-DFR2,OsCCRL1

• Symbol: OsTKPR1,OsDFR2,OS-DFR2,OsCCRL1
• MSU: LOC_Os09g32020
• RAPdb: Os09g0493500

• Conserved metabolic steps for sporopollenin precursor formation in tobacco and rice, 2013, Physiol Plant.
• Ostkpr1 functions in anther cuticle development and pollen wall formation in rice., 2019, BMC Plant Biol.
• OsCCRL1 is Essential for Phenylpropanoid Metabolism in Rice Anthers., 2023, Rice (N Y).

Genbank accession number

• Ostkpr1 functions in anther cuticle development and pollen wall formation in rice.
• Loss of function of OsTKPR1 delayed tapetum degradation, reduced the levels of anther cuticular lipids, and impaired Ubisch body and pollen exine formation, resulting in complete male sterility
• Localization studies suggest that OsTKPR1 accumulates in the endoplasmic reticulum, while specific accumulation of OsTKPR1 mRNA in the anther tapetum and microspores is consistent with its function in anther and pollen wall development
• Our results show that OsTKPR1 is indispensable for anther cuticle development and pollen wall formation in rice, providing new insights into the biochemical mechanisms of the conserved sporopollenin metabolon in flowering plants
• Furthermore, an R2R3 MYB transcription factor OsMYB103/OsMYB80/OsMS188/BM1, involved in tapetum and pollen development, regulates the expression of OsCCRL1
• In the current study, to investigate this, we identified and analyzed the male-sterile mutant, osccrl1 (cinnamoyl coA reductase-like 1), which exhibited delayed tapetal programmed cell death (PCD) and defective mature pollen
• Map-based cloning, genetic complementation, and gene knockout revealed that OsCCRL1 corresponds to the gene LOC_Os09g32020
• OsCCRL1 was preferentially expressed in the tapetal cells and microspores, and localized to the nucleus and cytoplasm in both rice protoplasts and Nicotiana benthamiana leaves
• The osccrl1 mutant exhibited reduced CCRs enzyme activity, less lignin accumulation, delayed tapetum degradation, and disrupted phenylpropanoid metabolism

• OsPKS1~YY2~OsLAP6, OsTKPR1~OsDFR2~OS-DFR2~OsCCRL1, Conserved metabolic steps for sporopollenin precursor formation in tobacco and rice, Similarly, recombinant rice OsPKS1 and OsTKPR1 were shown to function as downstream enzymes of NtACOS1
• OsPKS1~YY2~OsLAP6, OsTKPR1~OsDFR2~OS-DFR2~OsCCRL1, Conserved metabolic steps for sporopollenin precursor formation in tobacco and rice, Using fatty acids as starting substrates, sequential activities of heterologously expressed tobacco enzymes NtACOS1, NtPKS1 and NtTKPR1 resulted in the production of reduced tetraketide alpha-pyrones
• OsTKPR1~OsDFR2~OS-DFR2~OsCCRL1, OsTKPR2, OsTKPR2 is part of a sporopollenin-producing metabolon required for exine formation in rice., We found that OsTKPR2 and OsTKPR1 share a similar expression pattern, and their coding proteins have a similar subcellular localization and enzyme activities towards reduced tetraketide α-pyrone and hydroxylated tetraketide α-pyrone
• OsTKPR1~OsDFR2~OS-DFR2~OsCCRL1, OsTKPR2, OsTKPR2 is part of a sporopollenin-producing metabolon required for exine formation in rice., OsTKPR1 and OsTKPR2 were integrated into a metabolon including OsACOS12 and OsPKS2, which resulted in a significant increased enzymatic efficiency when both OsTKPR1 and OsTKPR2 were present, indicating the mutual dependence of OsTKPR2 and OsTKPR1 for their full biochemical activities
• OsTKPR1~OsDFR2~OS-DFR2~OsCCRL1, OsTKPR2, OsTKPR2 is part of a sporopollenin-producing metabolon required for exine formation in rice., Furthermore, the cooperation and possible functional divergence between OsTKPR2 and OsTKPR1 is also discussed