PAIR2

2015-01-20 | Categories genes  | Tags meiotic  meiosis  reproductive  pollen  vegetative 

• Information
  • Symbol: PAIR2
  • MSU: LOC_Os09g32930
  • RAPdb: Os09g0506800
• Publication
  • An insertional mutation in the rice PAIR2 gene, the ortholog of Arabidopsis ASY1, results in a defect in homologous chromosome pairing during meiosis, 2004, Mol Genet Genomics.
  • OsAM1 is required for leptotene-zygotene transition in rice, 2011, Cell Res.
  • PAIR2 is essential for homologous chromosome synapsis in rice meiosis I, 2006, J Cell Sci.
  • MER3 is required for normal meiotic crossover formation, but not for presynaptic alignment in rice, 2009, J Cell Sci.
  • OsSGO1 maintains synaptonemal complex stabilization in addition to protecting centromeric cohesion during rice meiosis, 2011, Plant J.
  • The role of OsCOM1 in homologous chromosome synapsis and recombination in rice meiosis, 2012, Plant J.
  • The central element protein ZEP1 of the synaptonemal complex regulates the number of crossovers during meiosis in rice, 2010, Plant Cell.
  • Central region component1, a novel synaptonemal complex component, is essential for meiotic recombination initiation in rice, 2013, Plant Cell.
  • OsREC8 is essential for chromatid cohesion and metaphase I monopolar orientation in rice meiosis, 2011, Plant Physiol.
  • OsSPO11-1 is essential for both homologous chromosome pairing and crossover formation in rice, 2010, Chromosoma.
  • Rice OsBRCA2 Is Required for DNA Double-Strand Break Repair in Meiotic Cells, 2020, Front Plant Sci.
• Genbank accession number
  • AB109238
• Key message
  • In the absence of OsAM1, many other critical meiotic components, including PAIR2, ZEP1 and OsMER3, could not be correctly installed onto chromosomes
  • In contrast, in pair2, Osmer3 and zep1 mutants, OsAM1 could be loaded normally, suggesting that OsAM1 plays a fundamental role in building the proper chromosome structure at the beginning of meiosis
  • Finally, we found that the centromeric localization of OsSGO1 depends on OsAM1, not other meiotic proteins such as OsREC8, PAIR2, OsMER3, or ZEP1
  • Mature PAIR2 mRNA and several splicing variants were found to be highly expressed in wild-type reproductive tissues, and lower expression was also detected in vegetative tissues
  • We found that CRC1 is also essential for the recruitment of PAIR2 onto meiotic chromosomes
  • In situ hybridization and BrdU incorporation experiments revealed that PAIR2 expression is specifically enhanced in male and female meiocytes, but not in those at pre-meiotic S phase or in the pollen maturation stages
  • The PAIR2 gene is required for homologous chromosome synapsis at meiosis I in rice (Oryza sativa L
  • However, PAIR2 does not play a role in AE formation, sister chromatid cohesion at centromeres or kinetochore assembly in meiosis I of rice
  • PAIR2 is essential for homologous chromosome synapsis in rice meiosis I
  • Several key meiotic proteins, including ZEP1 and OsMER3, were not loaded normally onto chromosomes in Oscom1 mutants, whereas the localization of OsREC8, PAIR2 and PAIR3 seemed to be normal
  • Moreover, OsCOM1 was loaded normally onto meiotic chromosomes in Osrec8, zep1 and Osmer3 mutants, but could not be properly loaded in Osam1, pair2 and OsSPO11-1(RNAi) plants
  • To elucidate the genetic system that establishes homologous chromosome pairing in monocot plants, we have isolated an asynaptic mutant of rice, designated pair2 (homologous pairing aberration in rice meiosis 2), in which 24 completely unpaired univalents are observed at pachytene and diakinesis
  • The results obtained in this study suggest that the PAIR2 gene is essential for homologous chromosome pairing in meiosis, as in the case of the genes ASY1 and HOP1
  • An insertional mutation in the rice PAIR2 gene, the ortholog of Arabidopsis ASY1, results in a defect in homologous chromosome pairing during meiosis
• Connection
  • OsRad21-4~OsREC8, PAIR2, OsSPO11-1 is essential for both homologous chromosome pairing and crossover formation in rice, Although the two axial-associated proteins, REC8 and PAIR2, are loaded onto the chromosomes, the depletion of PAIR2 from the chromosomes is much later than in wild type
  • OsAM1, PAIR2, OsAM1 is required for leptotene-zygotene transition in rice, In the absence of OsAM1, many other critical meiotic components, including PAIR2, ZEP1 and OsMER3, could not be correctly installed onto chromosomes
  • OsAM1, PAIR2, OsAM1 is required for leptotene-zygotene transition in rice, In contrast, in pair2, Osmer3 and zep1 mutants, OsAM1 could be loaded normally, suggesting that OsAM1 plays a fundamental role in building the proper chromosome structure at the beginning of meiosis
  • MER3~RCK, PAIR2, OsAM1 is required for leptotene-zygotene transition in rice, In the absence of OsAM1, many other critical meiotic components, including PAIR2, ZEP1 and OsMER3, could not be correctly installed onto chromosomes
  • MER3~RCK, PAIR2, OsAM1 is required for leptotene-zygotene transition in rice, In contrast, in pair2, Osmer3 and zep1 mutants, OsAM1 could be loaded normally, suggesting that OsAM1 plays a fundamental role in building the proper chromosome structure at the beginning of meiosis
  • PAIR2, ZEP1~OsZEP, OsAM1 is required for leptotene-zygotene transition in rice, In the absence of OsAM1, many other critical meiotic components, including PAIR2, ZEP1 and OsMER3, could not be correctly installed onto chromosomes
  • PAIR2, ZEP1~OsZEP, OsAM1 is required for leptotene-zygotene transition in rice, In contrast, in pair2, Osmer3 and zep1 mutants, OsAM1 could be loaded normally, suggesting that OsAM1 plays a fundamental role in building the proper chromosome structure at the beginning of meiosis
  • MER3~RCK, PAIR2, MER3 is required for normal meiotic crossover formation, but not for presynaptic alignment in rice, In addition, MER3 does not colocalize with PAIR2 at the beginning of prophase I, but locates on one end of PAIR2 fragments at later stages, whereas MER3 foci merely locate on one end of REC8 fragments when signals start to be seen in early prophase I
  • MER3~RCK, PAIR2, MER3 is required for normal meiotic crossover formation, but not for presynaptic alignment in rice, The normal loading of PAIR2 and REC8 in mer3 implies that their loading is independent of MER3
  • MER3~RCK, PAIR2, MER3 is required for normal meiotic crossover formation, but not for presynaptic alignment in rice, On the contrary, the absence of MER3 signal in pair2 mutants indicates that PAIR2 is essential for the loading and further function of MER3
  • OsRad21-4~OsREC8, PAIR2, MER3 is required for normal meiotic crossover formation, but not for presynaptic alignment in rice, In addition, MER3 does not colocalize with PAIR2 at the beginning of prophase I, but locates on one end of PAIR2 fragments at later stages, whereas MER3 foci merely locate on one end of REC8 fragments when signals start to be seen in early prophase I
  • OsRad21-4~OsREC8, PAIR2, MER3 is required for normal meiotic crossover formation, but not for presynaptic alignment in rice, The normal loading of PAIR2 and REC8 in mer3 implies that their loading is independent of MER3
  • OsAM1, PAIR2, OsSGO1 maintains synaptonemal complex stabilization in addition to protecting centromeric cohesion during rice meiosis, Finally, we found that the centromeric localization of OsSGO1 depends on OsAM1, not other meiotic proteins such as OsREC8, PAIR2, OsMER3, or ZEP1
  • PAIR2, ZEP1~OsZEP, OsSGO1 maintains synaptonemal complex stabilization in addition to protecting centromeric cohesion during rice meiosis, Finally, we found that the centromeric localization of OsSGO1 depends on OsAM1, not other meiotic proteins such as OsREC8, PAIR2, OsMER3, or ZEP1
  • OsSGO1, PAIR2, OsSGO1 maintains synaptonemal complex stabilization in addition to protecting centromeric cohesion during rice meiosis, Finally, we found that the centromeric localization of OsSGO1 depends on OsAM1, not other meiotic proteins such as OsREC8, PAIR2, OsMER3, or ZEP1
  • MER3~RCK, PAIR2, OsSGO1 maintains synaptonemal complex stabilization in addition to protecting centromeric cohesion during rice meiosis, Finally, we found that the centromeric localization of OsSGO1 depends on OsAM1, not other meiotic proteins such as OsREC8, PAIR2, OsMER3, or ZEP1
  • OsRad21-4~OsREC8, PAIR2, OsSGO1 maintains synaptonemal complex stabilization in addition to protecting centromeric cohesion during rice meiosis, Finally, we found that the centromeric localization of OsSGO1 depends on OsAM1, not other meiotic proteins such as OsREC8, PAIR2, OsMER3, or ZEP1
  • OsCOM1, PAIR2, The role of OsCOM1 in homologous chromosome synapsis and recombination in rice meiosis, Several key meiotic proteins, including ZEP1 and OsMER3, were not loaded normally onto chromosomes in Oscom1 mutants, whereas the localization of OsREC8, PAIR2 and PAIR3 seemed to be normal
  • OsCOM1, PAIR2, The role of OsCOM1 in homologous chromosome synapsis and recombination in rice meiosis, Moreover, OsCOM1 was loaded normally onto meiotic chromosomes in Osrec8, zep1 and Osmer3 mutants, but could not be properly loaded in Osam1, pair2 and OsSPO11-1(RNAi) plants
  • PAIR2, PAIR3, The role of OsCOM1 in homologous chromosome synapsis and recombination in rice meiosis, Several key meiotic proteins, including ZEP1 and OsMER3, were not loaded normally onto chromosomes in Oscom1 mutants, whereas the localization of OsREC8, PAIR2 and PAIR3 seemed to be normal
  • PAIR2, ZEP1~OsZEP, The role of OsCOM1 in homologous chromosome synapsis and recombination in rice meiosis, Several key meiotic proteins, including ZEP1 and OsMER3, were not loaded normally onto chromosomes in Oscom1 mutants, whereas the localization of OsREC8, PAIR2 and PAIR3 seemed to be normal
  • PAIR2, ZEP1~OsZEP, The role of OsCOM1 in homologous chromosome synapsis and recombination in rice meiosis, Moreover, OsCOM1 was loaded normally onto meiotic chromosomes in Osrec8, zep1 and Osmer3 mutants, but could not be properly loaded in Osam1, pair2 and OsSPO11-1(RNAi) plants
  • OsRad21-4~OsREC8, PAIR2, The role of OsCOM1 in homologous chromosome synapsis and recombination in rice meiosis, Several key meiotic proteins, including ZEP1 and OsMER3, were not loaded normally onto chromosomes in Oscom1 mutants, whereas the localization of OsREC8, PAIR2 and PAIR3 seemed to be normal
  • OsRad21-4~OsREC8, PAIR2, The role of OsCOM1 in homologous chromosome synapsis and recombination in rice meiosis, Moreover, OsCOM1 was loaded normally onto meiotic chromosomes in Osrec8, zep1 and Osmer3 mutants, but could not be properly loaded in Osam1, pair2 and OsSPO11-1(RNAi) plants
  • OsSPO11-1~OsTOP6A1, PAIR2, The role of OsCOM1 in homologous chromosome synapsis and recombination in rice meiosis, Moreover, OsCOM1 was loaded normally onto meiotic chromosomes in Osrec8, zep1 and Osmer3 mutants, but could not be properly loaded in Osam1, pair2 and OsSPO11-1(RNAi) plants
  • MER3~RCK, PAIR2, The role of OsCOM1 in homologous chromosome synapsis and recombination in rice meiosis, Several key meiotic proteins, including ZEP1 and OsMER3, were not loaded normally onto chromosomes in Oscom1 mutants, whereas the localization of OsREC8, PAIR2 and PAIR3 seemed to be normal
  • MER3~RCK, PAIR2, The role of OsCOM1 in homologous chromosome synapsis and recombination in rice meiosis, Moreover, OsCOM1 was loaded normally onto meiotic chromosomes in Osrec8, zep1 and Osmer3 mutants, but could not be properly loaded in Osam1, pair2 and OsSPO11-1(RNAi) plants
  • MER3~RCK, PAIR2, The central element protein ZEP1 of the synaptonemal complex regulates the number of crossovers during meiosis in rice, Although PAIR2 and MER3 were loaded normally in zep1, their dissociation was delayed severely compared with the wild type
  • PAIR2, ZEP1~OsZEP, The central element protein ZEP1 of the synaptonemal complex regulates the number of crossovers during meiosis in rice, Although PAIR2 and MER3 were loaded normally in zep1, their dissociation was delayed severely compared with the wild type
  • CRC1, PAIR2, Central region component1, a novel synaptonemal complex component, is essential for meiotic recombination initiation in rice, We found that CRC1 is also essential for the recruitment of PAIR2 onto meiotic chromosomes
  • OsRad21-4~OsREC8, PAIR2, OsREC8 is essential for chromatid cohesion and metaphase I monopolar orientation in rice meiosis, Immunolocalization analyses revealed that the loading of PAIR2, PAIR3, OsMER3, and ZEP1 all depended on OsREC8
  • OsRad21-4~OsREC8, PAIR2, OsREC8 is essential for chromatid cohesion and metaphase I monopolar orientation in rice meiosis, By contrast, the presence of the OsREC8 signal in pair2, pair3, Osmer3, and zep1 mutants indicated that the loading of OsREC8 did not rely on these four proteins
  • MER3~RCK, PAIR2, OsREC8 is essential for chromatid cohesion and metaphase I monopolar orientation in rice meiosis, Immunolocalization analyses revealed that the loading of PAIR2, PAIR3, OsMER3, and ZEP1 all depended on OsREC8
  • MER3~RCK, PAIR2, OsREC8 is essential for chromatid cohesion and metaphase I monopolar orientation in rice meiosis, By contrast, the presence of the OsREC8 signal in pair2, pair3, Osmer3, and zep1 mutants indicated that the loading of OsREC8 did not rely on these four proteins
  • PAIR2, ZEP1~OsZEP, OsREC8 is essential for chromatid cohesion and metaphase I monopolar orientation in rice meiosis, Immunolocalization analyses revealed that the loading of PAIR2, PAIR3, OsMER3, and ZEP1 all depended on OsREC8
  • PAIR2, ZEP1~OsZEP, OsREC8 is essential for chromatid cohesion and metaphase I monopolar orientation in rice meiosis, By contrast, the presence of the OsREC8 signal in pair2, pair3, Osmer3, and zep1 mutants indicated that the loading of OsREC8 did not rely on these four proteins
  • OsDMC1~DMC1B~OsDMC1B, PAIR2, OsDMC1 is not required for homologous pairing in rice meiosis., Moreover, OsDMC1 was not detected in pairing-defective mutants, such as pair2, pair3, Oscom1 and Osrad51c, while it was loaded onto meiotic chromosomes in zep1, Osmer3, Oszip4 and Oshei10
  • OsMTOPVIB, PAIR2, OsMTOPVIB is required for meiotic bipolar spindle assembly., We further revealed that the mono-orientation of sister kinetochores was prevalent in OsmtopVIB, whereas biorientation of sister kinetochores was frequently observed in Osspo11-1, pair2, and crc1 In addition, mutations of the cohesion subunit OsREC8 resulted in biorientation of sister kinetochores as well as bipolar spindles even in the background of OsmtopVIB Therefore, we propose that biorientation of the kinetochore is required for bipolar spindle assembly in the absence of homologous recombination

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