CPD,OsCPDP,qUVR-10

2015-01-20 | Categories genes  | Tags growth  resistant  breeding  mitochondria  chloroplast 

• Information
  • Symbol: CPD,OsCPDP,qUVR-10
  • MSU: LOC_Os10g08580
  • RAPdb: Os10g0167600
• Publication
  • qUVR-10, a major quantitative trait locus for ultraviolet-B resistance in rice, encodes cyclobutane pyrimidine dimer photolyase, 2005, Genetics.
  • Spontaneously occurring mutations in the cyclobutane pyrimidine dimer photolyase gene cause different sensitivities to ultraviolet-B in rice, 2005, Plant J.
  • Increase in CPD photolyase activity functions effectively to prevent growth inhibition caused by UVB radiation, 2007, Plant J.
  • Cyclobutane pyrimidine dimer CPD photolyase repairs ultraviolet-B-induced CPDs in rice chloroplast and mitochondrial DNA, 2011, Plant J.
  • UV radiation-sensitive norin 1 rice contains defective cyclobutane pyrimidine dimer photolyase, 2000, Plant Cell.
  • Ultraviolet-B sensitivities in Japanese lowland rice cultivars: cyclobutane pyrimidine dimer photolyase activity and gene mutation, 2004, Plant Cell Physiol.
  • The native cyclobutane pyrimidine dimer photolyase of rice is phosphorylated, 2008, Plant Physiol.
  • A gene for a Class II DNA photolyase from Oryza sativa: cloning of the cDNA by dilution-amplification, 2003, Mol Genet Genomics.
• Genbank accession number
  • AB096003
• Key message
  • UV-B radiation damages nuclear, chloroplast and mitochondrial DNA by the formation of cyclobutane pyrimidine dimers (CPDs), which are the primary UV-B-induced DNA lesions, and are a principal cause of UV-B-induced growth inhibition in plants
  • We previously indicated that UV-resistant rice cultivars are better able to repair cyclobutane pyrimidine dimers (CPDs) through photorepair than are UV-sensitive cultivars
  • Surjamkhi (indica) exhibited greater sensitivity to UVB radiation and was more deficient in CPD photorepair ability compared with UV-resistant Sasanishiki (japonica)
  • In order to verify that suggestion, we examined the correlation between UVB sensitivity and CPD photolyase activity in 17 rice cultivars of progenitors and relatives in breeding of UV-resistant Sasanishiki and UV-sensitive Norin 1
  • To date, the CPD repair processes in plant chloroplasts and mitochondria remain poorly understood
  • Here, we report the photoreactivation of CPDs in chloroplast and mitochondrial DNA in rice
  • Biochemical and subcellular localization analyses using rice strains with different levels of CPD photolyase activity and transgenic rice strains showed that full-length CPD photolyase is encoded by a single gene, not a splice variant, and is expressed and targeted not only to nuclei but also to chloroplasts and mitochondria
  • The results indicate that rice may have evolved a CPD photolyase that functions in chloroplasts, mitochondria and nuclei, and that contains DNA to protect cells from the harmful effects of UV-B radiation
  • Cyclobutane pyrimidine dimer (CPD) photolyase repairs ultraviolet-B-induced CPDs in rice chloroplast and mitochondrial DNA
  • 7-fold higher CPD photolyase activities than the wild-type, respectively, were significantly more resistant to UVB-induced growth damage, and maintained significantly lower CPD levels in their leaves during growth under elevated UVB radiation
  • Conversely, the AS-D plant had little photolyase activity, was severely damaged by elevated UVB radiation, and maintained higher CPD levels in its leaves during growth under UVB radiation
  • Notably, the S-C plant was not more resistant to UVB-induced growth inhibition than the S-B plant, even though it had much higher CPD photolyase activity
  • These results strongly indicate that UVB-induced CPDs are one of principal causes of UVB-induced growth inhibition in rice plants grown under supplementary UVB radiation, and that increasing CPD photolyase activity can significantly alleviate UVB-caused growth inhibition in rice
  • Increase in CPD photolyase activity functions effectively to prevent growth inhibition caused by UVB radiation
  • These results suggest that spontaneously occurring mutations in the CPD photolyase gene cause different degrees of sensitivity to UVB in rice, and that the resistance of rice to UVB radiation could be increased by increasing the photolyase function through conventional breeding or bioengineering
  • Plants homozygous for the Nipponbare allele at the qUVR-10 locus were more resistant to UVB compared with the Kasalath allele
  • Here, we tested whether CPD photolyase function determines the UVB sensitivity of rice (Oryza sativa) by generating transgenic rice plants bearing the CPD photolyase gene of the UV-resistant rice cultivar Sasanishiki in the sense orientation (S-B and S-C lines) or the antisense orientation (AS-D line)
• Connection
  • CPD~OsCPDP~qUVR-10, OsMeCP, Methyl-CpG binding domain protein acts to regulate the repair of cyclobutane pyrimidine dimers on rice DNA., Overexpression of OsMeCP led to lower Os10g0167600 transcript levels and a higher CPD content than in the WT, but the reverse was true in the OsMeCP-RNAi line
  • CPD~OsCPDP~qUVR-10, OsSLA1, OsSLA1 functions in leaf angle regulation by enhancing the interaction between OsBRI1 and OsBAK1 in rice., The OsSLA1 mutant plants were insensitive to exogenous epibrassinolide (eBL) and showed upregulated expression of DWARF and CPD, but downregulated expression of BU1, BUL1, and ILI1, indicating that brassinosteroid (BR) signal transduction was blocked

Prev     Next