- Information
- Symbol: OsGR3,GR3
- MSU: LOC_Os10g28000
- RAPdb: Os10g0415300
- Publication
- Na+ but not Cl- or osmotic stress is involved in NaCl-induced expression of Glutathione reductase in roots of rice seedlings, 2009, J Plant Physiol.
- Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings, 2012, J Plant Physiol.
- Identification and characterization of a novel chloroplast/mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice, 2013, Plant Mol Biol.
- NaCl-induced expression of glutathione reductase in roots of rice Oryza sativa L. seedlings is mediated through hydrogen peroxide but not abscisic acid, 2009, Plant and Soil.
- Gene knockout of glutathione reductase 3 results in increased sensitivity to salt stress in rice., 2015, Plant Mol Biol.
- Genbank accession number
- Key message
- In this work, we describe the cloning and characterization of the full-length cDNA encoding OsGR3, a chloroplast-localized GR that up to now was considered as a non-functional enzyme because of assumed lack of N-terminal conserved domains
- OsGR3 shows 76 and 53 % identity with OsGR1 (chloroplastic) and OsGR2 (cytosolic), respectively
- A plastid transit peptide is located at the N terminus of OsGR3, and genetic transformation of rice with a GR3-GFP fusion construct further confirmed its localization in chloroplasts
- Furthermore, OsGR1 and OsGR3 are also targeted to mitochondria, which suggest a combined antioxidant mechanism in both chloroplasts and mitochondria
- In addition, the transcript level of OsGR3 was greatly increased with salicylic acid treatment but was not significantly affected by methyl jasmonate, dehydration or heat shock stress
- In fact, exogenous application of ABA enhanced the expression of OsGR2 and OsGR3 in rice roots
- On inhibiting ABA accumulation with sodium tungstate (Tu), an inhibitor of ABA biosynthesis, the expression of OsGR2 and OsGR3 was still induced by NaCl; therefore, NaCl-triggered expression of OsGR2 and OsGR3 in rice roots is not mediated by accumulation of ABA
- However, both isoforms showed a distinct response to salinity: the expression of OsGR3 but not OsGR1 was induced by salt stress
- Our results provide new clues about the possible roles of functional OsGR3 in salt stress and biotic stress tolerance
- Semi-quantitative RT-PCR was used to quantify the mRNA levels for one cytosolic (OsGR2) and two chloroplastic (OsGR1 and OsGR3) isoforms of GR identified in the rice genome
- Semi-quantitative RT-PCR was applied to quantify the mRNA levels for one cytosolic (OsGR2) and two chloroplastic (OsGR1 and OsGR3) isoforms of glutathione reductase identified in the rice genome
- The expression of OsGR2 and OsGR3 but not OsGR1 was increased in rice roots treated with NaCl
- NaCl-induced OsGR2 and OsGR3 in rice roots could be associated with Na(+) but not an osmotic component
- The expression of OsGR2 and OsGR3 but not OsGR1 was increased in rice roots treated with 150 mM NaCl
- However, NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction
- Moreover, the increase in H2O2 level was prior to the induction of OsGR2 and OsGR3 in NaCl-treated rice roots
- Thus, H2O2, but not ABA, is involved in regulation of OsGR2 and OsGR3 expression in NaCl-treated rice roots
- GR3 promoter-GUS was expressed in the vascular cylinder and cortex of root tissues in rice seedlings, vascular tissue of nodes, embryo and aleurone layer of seeds, and young flowers
- Previously, we showed that salt-stress-responsive GR3 is a functional protein localized in chloroplasts and mitochondria in rice
- Rice GR3 was primarily expressed in roots at the seedling stage and ubiquitously expressed in all tissues except the sheath at heading stage
- Oxidative stress, indicated by malondialdehyde content, was greater in gr3 than the WT under salt stress
- As compared with the WT, gr3 was sensitive to salt and methyl viologen; it showed inhibited growth, decreased maximal efficiency of photosystem II, decreased GSH and GSSG contents, and the ratio of GSH to GSSG
- These results reveal that GR3 plays an important role in salt stress tolerance by regulating the GSH redox state in rice
- To learn more about the role of GR3 in salt-stress tolerance, we investigated the response to 100 mM NaCl treatment in wild-type rice (WT); GR3 knockout mutant of rice (gr3); and the functional gr3-complementation line (C1)
- Connection
- OsGR1, OsGR3~GR3, Na+ but not Cl- or osmotic stress is involved in NaCl-induced expression of Glutathione reductase in roots of rice seedlings, Semi-quantitative RT-PCR was used to quantify the mRNA levels for one cytosolic (OsGR2) and two chloroplastic (OsGR1 and OsGR3) isoforms of GR identified in the rice genome
- OsGR1, OsGR3~GR3, Na+ but not Cl- or osmotic stress is involved in NaCl-induced expression of Glutathione reductase in roots of rice seedlings, The expression of OsGR2 and OsGR3 but not OsGR1 was increased in rice roots treated with NaCl
- OsGR2~RGRC2, OsGR3~GR3, Na+ but not Cl- or osmotic stress is involved in NaCl-induced expression of Glutathione reductase in roots of rice seedlings, Semi-quantitative RT-PCR was used to quantify the mRNA levels for one cytosolic (OsGR2) and two chloroplastic (OsGR1 and OsGR3) isoforms of GR identified in the rice genome
- OsGR2~RGRC2, OsGR3~GR3, Na+ but not Cl- or osmotic stress is involved in NaCl-induced expression of Glutathione reductase in roots of rice seedlings, The expression of OsGR2 and OsGR3 but not OsGR1 was increased in rice roots treated with NaCl
- OsGR2~RGRC2, OsGR3~GR3, Na+ but not Cl- or osmotic stress is involved in NaCl-induced expression of Glutathione reductase in roots of rice seedlings, Treatment with 150 mM NaCl and 150 mM NaNO(3) affected OsGR2 and OsGR3 induction similarly, which suggests that Na(+) but not Cl(-) is required for the NaCl-induced expression of OsGR2 and OsGR3
- OsGR2~RGRC2, OsGR3~GR3, Na+ but not Cl- or osmotic stress is involved in NaCl-induced expression of Glutathione reductase in roots of rice seedlings, Here, we show that OsGR2 and OsGR3 expression, GR activity, and H(2)O(2) content were not affected at a concentration of mannitol iso-osmotic with 150 mM NaCl
- OsGR2~RGRC2, OsGR3~GR3, Na+ but not Cl- or osmotic stress is involved in NaCl-induced expression of Glutathione reductase in roots of rice seedlings, NaCl-induced OsGR2 and OsGR3 in rice roots could be associated with Na(+) but not an osmotic component
- OsGR2~RGRC2, OsGR3~GR3, NaCl-induced expression of glutathione reductase in roots of rice Oryza sativa L. seedlings is mediated through hydrogen peroxide but not abscisic acid, Semi-quantitative RT-PCR was applied to quantify the mRNA levels for one cytosolic (OsGR2) and two chloroplastic (OsGR1 and OsGR3) isoforms of glutathione reductase identified in the rice genome
- OsGR2~RGRC2, OsGR3~GR3, NaCl-induced expression of glutathione reductase in roots of rice Oryza sativa L. seedlings is mediated through hydrogen peroxide but not abscisic acid, The expression of OsGR2 and OsGR3 but not OsGR1 was increased in rice roots treated with 150 mM NaCl
- OsGR2~RGRC2, OsGR3~GR3, NaCl-induced expression of glutathione reductase in roots of rice Oryza sativa L. seedlings is mediated through hydrogen peroxide but not abscisic acid, In fact, exogenous application of ABA enhanced the expression of OsGR2 and OsGR3 in rice roots
- OsGR2~RGRC2, OsGR3~GR3, NaCl-induced expression of glutathione reductase in roots of rice Oryza sativa L. seedlings is mediated through hydrogen peroxide but not abscisic acid, On inhibiting ABA accumulation with sodium tungstate (Tu), an inhibitor of ABA biosynthesis, the expression of OsGR2 and OsGR3 was still induced by NaCl; therefore, NaCl-triggered expression of OsGR2 and OsGR3 in rice roots is not mediated by accumulation of ABA
- OsGR2~RGRC2, OsGR3~GR3, NaCl-induced expression of glutathione reductase in roots of rice Oryza sativa L. seedlings is mediated through hydrogen peroxide but not abscisic acid, However, NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction
- OsGR2~RGRC2, OsGR3~GR3, NaCl-induced expression of glutathione reductase in roots of rice Oryza sativa L. seedlings is mediated through hydrogen peroxide but not abscisic acid, On inhibiting the NaCl-induced accumulation of H2O2 with diphenylene iodonium, the expression of OsGR2 and OsGR3 was also suppressed
- OsGR2~RGRC2, OsGR3~GR3, NaCl-induced expression of glutathione reductase in roots of rice Oryza sativa L. seedlings is mediated through hydrogen peroxide but not abscisic acid, Moreover, the increase in H2O2 level was prior to the induction of OsGR2 and OsGR3 in NaCl-treated rice roots
- OsGR2~RGRC2, OsGR3~GR3, NaCl-induced expression of glutathione reductase in roots of rice Oryza sativa L. seedlings is mediated through hydrogen peroxide but not abscisic acid, Thus, H2O2, but not ABA, is involved in regulation of OsGR2 and OsGR3 expression in NaCl-treated rice roots
- OsGR1, OsGR3~GR3, NaCl-induced expression of glutathione reductase in roots of rice Oryza sativa L. seedlings is mediated through hydrogen peroxide but not abscisic acid, Semi-quantitative RT-PCR was applied to quantify the mRNA levels for one cytosolic (OsGR2) and two chloroplastic (OsGR1 and OsGR3) isoforms of glutathione reductase identified in the rice genome
- OsGR1, OsGR3~GR3, NaCl-induced expression of glutathione reductase in roots of rice Oryza sativa L. seedlings is mediated through hydrogen peroxide but not abscisic acid, The expression of OsGR2 and OsGR3 but not OsGR1 was increased in rice roots treated with 150 mM NaCl
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