OsPht1;3,OsPT3

2015-01-20 | Categories genes  | Tags pi  phosphate  leaf  vascular bundle  shoot  phloem  Pi  phosphorus  Pi uptake 

• Information
  • Symbol: OsPht1;3,OsPT3
  • MSU: LOC_Os10g30770
  • RAPdb: Os10g0444600
• Publication
  • Involvement of OsSPX1 in phosphate homeostasis in rice, 2009, Plant J.
  • Rice phosphate transporters include an evolutionarily divergent gene specifically activated in arbuscular mycorrhizal symbiosis, 2002, Proc Natl Acad Sci U S A.
  • OsPHT1;3 Mediates Uptake, Translocation and Remobilization of Phosphate under Extremely Low Phosphate Regimes., 2018, Plant Physiol.
• Genbank accession number
  • AF239619
  • AF536963
  • AF239619
  • AF536963
• Key message
  • In contrast, overexpression of OsSPX1 suppressed the induction of expression by Pi starvation of all 10 phosphate starvation-induced genes tested: IPS1 (induced by phosphate starvation 1), IPS2, OsPAP10 (purple acid phosphatase 10), OsSQD2 (sulfoquinovosyldiacylglycerol 2), miR399d and miR399j (microRNA 399), OsPT2, OsPT3, OsPT6 and OsPT8
  • OsPHT1;3 was directly regulated by OsPHR2 and, in response to Pi starvation, showed enhanced expression in young leaf blades and shoot basal regions and even more so in roots and old leaf blades
  • Moreover, in basal nodes, the expression of OsPHT1;3 was restricted to the phloem of regular vascular bundles and enlarged vascular bundles
  • OsPHT1;3 Mediates Uptake, Translocation and Remobilization of Phosphate under Extremely Low Phosphate Regimes.
  • OsPHT1;3 was able to complement a yeast mutant strain defective in five Pi transporters and mediate Pi influx in Xenopus laevis oocytes
  • Overexpression of OsPHT1;3 led to increased Pi concentration both in roots and shoots
  • However, unlike that reported for other known OsPHT1 members which facilitate Pi uptake at relatively higher Pi levels, mutation of OsPHT1;3 impaired Pi uptake and root-to-shoot Pi translocation only when external Pi concentration was below 5 M
  • An isotope labeling experiment with 32P showed that ospht1;3 mutant lines were impaired in remobilization of Pi from source to sink leaves
  • Taken together, our results indicate that OsPHT1;3 acts as a crucial factor for Pi acquisition, root-to-shoot Pi translocation, and redistribution of phosphorus in plants growing in environments with extremely low Pi levels
• Connection
  • OsPht1;3~OsPT3, OsPht1;8~OsPT8, Involvement of OsSPX1 in phosphate homeostasis in rice, In contrast, overexpression of OsSPX1 suppressed the induction of expression by Pi starvation of all 10 phosphate starvation-induced genes tested: IPS1 (induced by phosphate starvation 1), IPS2, OsPAP10 (purple acid phosphatase 10), OsSQD2 (sulfoquinovosyldiacylglycerol 2), miR399d and miR399j (microRNA 399), OsPT2, OsPT3, OsPT6 and OsPT8
  • OsPht1;3~OsPT3, OsSPX1, Involvement of OsSPX1 in phosphate homeostasis in rice, In contrast, overexpression of OsSPX1 suppressed the induction of expression by Pi starvation of all 10 phosphate starvation-induced genes tested: IPS1 (induced by phosphate starvation 1), IPS2, OsPAP10 (purple acid phosphatase 10), OsSQD2 (sulfoquinovosyldiacylglycerol 2), miR399d and miR399j (microRNA 399), OsPT2, OsPT3, OsPT6 and OsPT8
  • OsPht1;2~OsPT2, OsPht1;3~OsPT3, Involvement of OsSPX1 in phosphate homeostasis in rice, In contrast, overexpression of OsSPX1 suppressed the induction of expression by Pi starvation of all 10 phosphate starvation-induced genes tested: IPS1 (induced by phosphate starvation 1), IPS2, OsPAP10 (purple acid phosphatase 10), OsSQD2 (sulfoquinovosyldiacylglycerol 2), miR399d and miR399j (microRNA 399), OsPT2, OsPT3, OsPT6 and OsPT8
  • OsLPT1~OsPht1;6~OsPT6, OsPht1;3~OsPT3, Involvement of OsSPX1 in phosphate homeostasis in rice, In contrast, overexpression of OsSPX1 suppressed the induction of expression by Pi starvation of all 10 phosphate starvation-induced genes tested: IPS1 (induced by phosphate starvation 1), IPS2, OsPAP10 (purple acid phosphatase 10), OsSQD2 (sulfoquinovosyldiacylglycerol 2), miR399d and miR399j (microRNA 399), OsPT2, OsPT3, OsPT6 and OsPT8
  • OsPht1;3~OsPT3, OsSQD2~OsSQD2.2, Involvement of OsSPX1 in phosphate homeostasis in rice, In contrast, overexpression of OsSPX1 suppressed the induction of expression by Pi starvation of all 10 phosphate starvation-induced genes tested: IPS1 (induced by phosphate starvation 1), IPS2, OsPAP10 (purple acid phosphatase 10), OsSQD2 (sulfoquinovosyldiacylglycerol 2), miR399d and miR399j (microRNA 399), OsPT2, OsPT3, OsPT6 and OsPT8
  • OsPht1;3~OsPT3, OsPIE1, A phosphate-starvation induced RING-type E3 ligase maintains phosphate homeostasis partially through OsSPX2 in rice., Under Pi-sufficient condition, overexpression of OsPIE1 upregulated the expression of OsPT2, OsPT3, OsPT10 and OsPAP21b, resulting in Pi accumulation and acid phosphatases (APases) induction in roots
  • OsPHR2, OsPht1;3~OsPT3, OsPHT1;3 Mediates Uptake, Translocation and Remobilization of Phosphate under Extremely Low Phosphate Regimes., OsPHT1;3 was directly regulated by OsPHR2 and, in response to Pi starvation, showed enhanced expression in young leaf blades and shoot basal regions and even more so in roots and old leaf blades
  • OsPHT1, OsPht1;3~OsPT3, OsPHT1;3 Mediates Uptake, Translocation and Remobilization of Phosphate under Extremely Low Phosphate Regimes., However, unlike that reported for other known OsPHT1 members which facilitate Pi uptake at relatively higher Pi levels, mutation of OsPHT1;3 impaired Pi uptake and root-to-shoot Pi translocation only when external Pi concentration was below 5 μM
  • OsPHT1, OsPht1;3~OsPT3, OsPHT1;3 Mediates Uptake, Translocation and Remobilization of Phosphate under Extremely Low Phosphate Regimes., Furthermore, overexpression and mutation of OsPHT1;3 led to reciprocal alteration in the expression of OsPHT1;2 and several other OsPHT1 genes
  • OsPht1;2~OsPT2, OsPht1;3~OsPT3, OsPHT1;3 Mediates Uptake, Translocation and Remobilization of Phosphate under Extremely Low Phosphate Regimes., Furthermore, overexpression and mutation of OsPHT1;3 led to reciprocal alteration in the expression of OsPHT1;2 and several other OsPHT1 genes
  • OsPht1;2~OsPT2, OsPht1;3~OsPT3, OsPHT1;3 Mediates Uptake, Translocation and Remobilization of Phosphate under Extremely Low Phosphate Regimes., Yeast-two-hybrid, bimolecular fluorescence complementation, and co-immunoprecipitation assays all demonstrated a physical interaction between OsPHT1;3 and OsPHT1;2

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