OsDUR3

2015-01-20 | Categories genes  | Tags nitrogen  transporter  growth  root 

• Information
  • Symbol: OsDUR3
  • MSU: LOC_Os10g42960
  • RAPdb: Os10g0580400
• Publication
  • Rice DUR3 mediates high-affinity urea transport and plays an effective role in improvement of urea acquisition and utilization when expressed in Arabidopsis, 2012, New Phytol.
• Genbank accession number
  • AY463691
• Key message
  • • Quantitative reverse-transcription polymerase chain reaction (qPCR) analysis revealed upregulation of OsDUR3 in rice roots under nitrogen-deficiency and urea-resupply after nitrogen-starvation
  • • A high-affinity urea transporter OsDUR3 from rice was functionally characterized here for the first time among crops
  • Together with its plasma membrane localization detected by green fluorescent protein (GFP)-tagging and with findings that disruption of OsDUR3 by T-DNA reduces rice growth on urea and urea uptake, we suggest that OsDUR3 is an active urea transporter that plays a significant role in effective urea acquisition and utilisation in rice
  • Heterologous expression demonstrated that OsDUR3 restored yeast dur3-mutant growth on urea and facilitated urea import with a K(m) of c
  • Importantly, overexpression of OsDUR3 complemented the Arabidopsis atdur3-1 mutant, improving growth on low urea and increasing root urea-uptake markedly
• Connection

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