- Information
- Symbol: NAL2,OsWOX3A,WOX3
- MSU: LOC_Os11g01130
- RAPdb: Os11g0102100
- Publication
- The rice narrow leaf2 and narrow leaf3 loci encode WUSCHEL-related homeobox 3A OsWOX3A and function in leaf, spikelet, tiller and lateral root development, 2013, New Phytol.
- Two WUSCHEL-related homeobox genes, narrow leaf2 and narrow leaf3, control leaf width in rice, 2013, Plant Cell Physiol.
- A WUSCHEL-LIKE HOMEOBOX gene represses a YABBY gene expression required for rice leaf development, 2007, Plant Physiol.
- OsWOX3A is involved in negative feedback regulation of the gibberellic acid biosynthetic pathway in rice Oryza sativa., 2016, J Exp Bot.
- Regulatory role of the OsWOX3A transcription factor in rice root development., 2016, Plant Signal Behav.
- Characterization of dwarf and narrow leaf dnl-4 mutant in rice, 2020, Plant Signal Behav.
- Narrow Leaf21, Encoding Ribosomal Protein RPS3A, Controls Leaf Development in Rice, 2021, Plant Physiol.
- Genbank accession number
- Key message
- In order to understand the molecular genetic mechanisms of rice (Oryza sativa) organ development, we studied the narrow leaf2 narrow leaf3 (nal2 nal3; hereafter nal2/3) double mutant, which produces narrow-curly leaves, more tillers, fewer lateral roots, opened spikelets and narrow-thin grains
- Expression levels of several leaf development-associated genes were altered in nal2/3, and auxin transport-related genes were significantly changed, leading to pin mutant-like phenotypes such as more tillers and fewer lateral roots
- Map-based cloning revealed that NAL2 and NAL3 are paralogs that encode an identical OsWOX3A (OsNS) transcriptional activator, homologous to NARROW SHEATH1 (NS1) and NS2 in maize and PRESSED FLOWER in Arabidopsis
- OsWOX3A is involved in organ development in rice, lateral-axis outgrowth and vascular patterning in leaves, lemma and palea morphogenesis in spikelets, and development of tillers and lateral roots
- The rice narrow leaf2 and narrow leaf3 loci encode WUSCHEL-related homeobox 3A (OsWOX3A) and function in leaf, spikelet, tiller and lateral root development
- Thus, NAL2/3 can be used to modulate leaf shape and improve agronomic yield in crop plants
- Rice YAB3 is closely related to maize (Zea mays) ZmYAB14 and Arabidopsis (Arabidopsis thaliana) FILAMENTOUS FLOWER (FIL), whereas rice WOX3 is highly conserved with maize narrow sheath1 (NS1) and NS2 and Arabidopsis PRESSED FLOWER (PRS)
- Here, we report the characterization of functional relationship between rice (Oryza sativa) YAB3 and WOX3 in rice leaf development
- These data reveal a regulatory network involving YAB3, WOX3, and KNOX genes required for rice leaf development
- The narrow-leaf phenotype of FL90 shows a two-factor recessive inheritance and is caused by the loss of function of two WUSCHEL-related homeobox genes, NAL2 and NAL3 (NAL2/3), which are duplicate genes orthologous to maize NS1 and NS2 and to Arabidopsis PRS
- The overexpression of NAL2/3 in transgenic rice plants results in wider leaves containing increased numbers of veins, suggesting that NAL2/3 expression regulates leaf width
- These data indicate that OsWOX3A is a GA-responsive gene and functions in the negative feedback regulation of the GA biosynthetic pathway for GA homeostasis to maintain the threshold levels of endogenous GA intermediates throughout development
- Exogenous GA3 treatment fully rescued the developmental defects of OsWOX3A-OX plants, suggesting that constitutive overexpression of OsWOX3A downregulates GA biosynthesis
- OsWOX3A expression is drastically and temporarily upregulated by GA3 and downregulated by paclobutrazol, a blocker of GA biosynthesis
- Among the rice (Oryza sativa) WOX proteins, a loss of OsWOX3A function in narrow leaf2 (nal2) nal3 double mutants (termed nal2/3) causes pleiotropic effects, such as narrow and curly leaves, opened spikelets, narrow grains, more tillers, and fewer lateral roots, but almost normal plant height
- Regulatory role of the OsWOX3A transcription factor in rice root development.
- These findings indicate that OsWOX3A may be involved in modulation of GA-auxin crosstalk in rice root development
- In a recent study, we demonstrated that OsWOX3A directly binds to the promoter of the KAO gene, which encodes ent-kaurenoic acid oxidase, an enzyme involved in GA biosynthesis, and represses KAO expression
- Interestingly, we observed that OsWOX3A overexpression causes not only severe dwarfism, but also an increase in the number of lateral roots
- Transformation with modified OsARF11, OsARF16 and OsWOX3A genomic DNA lacking uORFs rescued the narrow leaf phenotype of nal21 to a better extent than transformation with their native genomic DNA, implying that RPS3A could regulate translation of ARFs and WOX3A through uORFs
- Connection
- NAL2~OsWOX3A~WOX3, NAL3, The rice narrow leaf2 and narrow leaf3 loci encode WUSCHEL-related homeobox 3A OsWOX3A and function in leaf, spikelet, tiller and lateral root development, In order to understand the molecular genetic mechanisms of rice (Oryza sativa) organ development, we studied the narrow leaf2 narrow leaf3 (nal2 nal3; hereafter nal2/3) double mutant, which produces narrow-curly leaves, more tillers, fewer lateral roots, opened spikelets and narrow-thin grains
- NAL2~OsWOX3A~WOX3, NAL3, The rice narrow leaf2 and narrow leaf3 loci encode WUSCHEL-related homeobox 3A OsWOX3A and function in leaf, spikelet, tiller and lateral root development, Map-based cloning revealed that NAL2 and NAL3 are paralogs that encode an identical OsWOX3A (OsNS) transcriptional activator, homologous to NARROW SHEATH1 (NS1) and NS2 in maize and PRESSED FLOWER in Arabidopsis
- NAL2~OsWOX3A~WOX3, NAL3, Two WUSCHEL-related homeobox genes, narrow leaf2 and narrow leaf3, control leaf width in rice, The narrow-leaf phenotype of FL90 shows a two-factor recessive inheritance and is caused by the loss of function of two WUSCHEL-related homeobox genes, NAL2 and NAL3 (NAL2/3), which are duplicate genes orthologous to maize NS1 and NS2 and to Arabidopsis PRS
- NAL2~OsWOX3A~WOX3, OsYABBY5~OsYAB3~TOB1, A WUSCHEL-LIKE HOMEOBOX gene represses a YABBY gene expression required for rice leaf development, Here, we report the characterization of functional relationship between rice (Oryza sativa) YAB3 and WOX3 in rice leaf development
- NAL2~OsWOX3A~WOX3, OsYABBY5~OsYAB3~TOB1, A WUSCHEL-LIKE HOMEOBOX gene represses a YABBY gene expression required for rice leaf development, Rice YAB3 is closely related to maize (Zea mays) ZmYAB14 and Arabidopsis (Arabidopsis thaliana) FILAMENTOUS FLOWER (FIL), whereas rice WOX3 is highly conserved with maize narrow sheath1 (NS1) and NS2 and Arabidopsis PRESSED FLOWER (PRS)
- NAL2~OsWOX3A~WOX3, OsYABBY5~OsYAB3~TOB1, A WUSCHEL-LIKE HOMEOBOX gene represses a YABBY gene expression required for rice leaf development, Overexpression of WOX3 repressed YAB3 and showed a YAB3 RNAi phenotype
- NAL2~OsWOX3A~WOX3, OsYABBY5~OsYAB3~TOB1, A WUSCHEL-LIKE HOMEOBOX gene represses a YABBY gene expression required for rice leaf development, The expression of class I KNOTTED-LIKE HOMEOBOX (KNOX) genes was ectopically induced in leaves of YAB3 RNAi or WOX3 overexpression plants
- NAL2~OsWOX3A~WOX3, OsYABBY5~OsYAB3~TOB1, A WUSCHEL-LIKE HOMEOBOX gene represses a YABBY gene expression required for rice leaf development, Data from inducible WOX3 expression and DNA-protein interaction assays suggested that WOX3 acted as a transcriptional repressor of YAB3
- NAL2~OsWOX3A~WOX3, OsYABBY5~OsYAB3~TOB1, A WUSCHEL-LIKE HOMEOBOX gene represses a YABBY gene expression required for rice leaf development, These data reveal a regulatory network involving YAB3, WOX3, and KNOX genes required for rice leaf development
- KAO, NAL2~OsWOX3A~WOX3, OsWOX3A is involved in negative feedback regulation of the gibberellic acid biosynthetic pathway in rice Oryza sativa., Yeast one-hybrid and electrophoretic mobility shift assays revealed that OsWOX3A directly interacts with the KAO promoter
- NAL2~OsWOX3A~WOX3, NAL3, OsWOX3A is involved in negative feedback regulation of the gibberellic acid biosynthetic pathway in rice Oryza sativa., Among the rice (Oryza sativa) WOX proteins, a loss of OsWOX3A function in narrow leaf2 (nal2) nal3 double mutants (termed nal2/3) causes pleiotropic effects, such as narrow and curly leaves, opened spikelets, narrow grains, more tillers, and fewer lateral roots, but almost normal plant height
- KAO, NAL2~OsWOX3A~WOX3, Regulatory role of the OsWOX3A transcription factor in rice root development., In a recent study, we demonstrated that OsWOX3A directly binds to the promoter of the KAO gene, which encodes ent-kaurenoic acid oxidase, an enzyme involved in GA biosynthesis, and represses KAO expression
- NAL2~OsWOX3A~WOX3, OsFMO~OsCOW1~NAL7~OsYUC8, Genetic analysis of rice mutants responsible for narrow leaf phenotype and reduced vein number., In this paper, we examined the function of four genes that regulate the width of the leaf blade and the vein number: NARROW LEAF1 (NAL1), NAL2, NAL3 and NAL7
- NAL2~OsWOX3A~WOX3, OsFMO~OsCOW1~NAL7~OsYUC8, Genetic analysis of rice mutants responsible for narrow leaf phenotype and reduced vein number., The nal7 mutation showed additive effects on both leaf width and vein number, when combined with the nal1 single or the nal2 nal3 double mutation
- NAL2~OsWOX3A~WOX3, OsFMO~OsCOW1~NAL7~OsYUC8, Genetic analysis of rice mutants responsible for narrow leaf phenotype and reduced vein number., In addition, observations of inner tissues revealed that cell differentiation was partially compromised in the nal2 nal3 nal7 mutant, consistent with the severe reduction in leaf width in this triple mutant
- GLR1~OsWOX3B~GL1~LSY1, NAL2~OsWOX3A~WOX3, LEAF LATERAL SYMMETRY1, a member of the WUSCHEL-RELATED HOMEOBOX3 gene family, regulates lateral outgrowth of leaves in rice., In this study, we show that a WOX3 gene, LEAF LATERAL SYMMETRY1 (LSY1), regulates lateral leaf development in a different manner than that of other duplicated paralogs of WOX3, NARROW LEAF2 (NAL2)/NAL3, in rice
- GLR1~OsWOX3B~GL1~LSY1, NAL2~OsWOX3A~WOX3, LEAF LATERAL SYMMETRY1, a member of the WUSCHEL-RELATED HOMEOBOX3 gene family, regulates lateral outgrowth of leaves in rice., Our results indicated that all of the rice WOX3 genes are involved in leaf lateral outgrowth, but the functions of LSY1 and NAL2/3 have diverged
- NAL2~OsWOX3A~WOX3, OsCR4~MIS2, Receptor-like kinase OsCR4 controls leaf morphogenesis and embryogenesis by fixing the distribution of auxin in rice, OsWOX3A, which is auxin responsive and related to leaf development, was activated extensively and ectopically in oscr4 leaves, partially accounting for the observed lack of cell differentiation
- NAL2~OsWOX3A~WOX3, OsARF11, Narrow Leaf21, Encoding Ribosomal Protein RPS3A, Controls Leaf Development in Rice, Transformation with modified OsARF11, OsARF16 and OsWOX3A genomic DNA lacking uORFs rescued the narrow leaf phenotype of nal21 to a better extent than transformation with their native genomic DNA, implying that RPS3A could regulate translation of ARFs and WOX3A through uORFs
- NAL2~OsWOX3A~WOX3, OsARF16, Narrow Leaf21, Encoding Ribosomal Protein RPS3A, Controls Leaf Development in Rice, Transformation with modified OsARF11, OsARF16 and OsWOX3A genomic DNA lacking uORFs rescued the narrow leaf phenotype of nal21 to a better extent than transformation with their native genomic DNA, implying that RPS3A could regulate translation of ARFs and WOX3A through uORFs
- NAL2~OsWOX3A~WOX3, NAL21, Narrow Leaf21, Encoding Ribosomal Protein RPS3A, Controls Leaf Development in Rice, Transformation with modified OsARF11, OsARF16 and OsWOX3A genomic DNA lacking uORFs rescued the narrow leaf phenotype of nal21 to a better extent than transformation with their native genomic DNA, implying that RPS3A could regulate translation of ARFs and WOX3A through uORFs
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