- Information
- Symbol: D53
- MSU: LOC_Os11g01330
- RAPdb: Os11g0104300,Os11g0104350
- Publication
- DWARF 53 acts as a repressor of strigolactone signalling in rice, 2013, Nature.
- D14-SCFD3-dependent degradation of D53 regulates strigolactone signalling, 2013, Nature.
- A D53 repression motif induces oligomerization of TOPLESS corepressors and promotes assembly of a corepressor-nucleosome complex., 2017, Sci Adv.
- Characterization of dwarf and narrow leaf dnl-4 mutant in rice, 2020, Plant Signal Behav.
- Sucrose promotes D53 accumulation and tillering in rice., 2021, New Phytol.
- Strigolactone and gibberellin signaling coordinately regulate metabolic adaptations to changes in nitrogen availability in rice., 2023, Mol Plant.
- Genbank accession number
- Key message
- D14-SCF(D3)-dependent degradation of D53 regulates strigolactone signalling
- We demonstrate that, in a D14- and D3-dependent manner, SLs induce D53 degradation by the proteasome and abrogate its activity in promoting axillary bud outgrowth
- Here we report the characterization of a dominant SL-insensitive rice (Oryza sativa) mutant dwarf 53 (d53) and the cloning of D53, which encodes a substrate of the SCF(D3) ubiquitination complex and functions as a repressor of SL signalling
- Here we show that DWARF 53 (D53) acts as a repressor of SL signalling and that SLs induce its degradation
- The D53 gene product shares predicted features with the class I Clp ATPase proteins and can form a complex with the alpha/beta hydrolase protein DWARF 14 (D14) and the F-box protein DWARF 3 (D3), two previously identified signalling components potentially responsible for SL perception
- We find that the rice (Oryza sativa) d53 mutant, which produces an exaggerated number of tillers compared to wild-type plants, is caused by a gain-of-function mutation and is insensitive to exogenous SL treatment
- Treatments with GR24, a synthetic SL analogue, cause D53 degradation via the proteasome in a manner that requires D14 and the SCF(D3) ubiquitin ligase, whereas the dominant form of D53 is resistant to SL-mediated degradation
- Sucrose promotes D53 accumulation and tillering in rice.
- Overexpression of D3 antagonizes sucrose inhibition of D53 degradation and enables the SL inhibition of tillering under high sucrose
- Sucrose prevents SL-induced degradation of D14, the SL receptor involved in D53 degradation
- Connection
- D3, D53, DWARF 53 acts as a repressor of strigolactone signalling in rice, Here we report the characterization of a dominant SL-insensitive rice (Oryza sativa) mutant dwarf 53 (d53) and the cloning of D53, which encodes a substrate of the SCF(D3) ubiquitination complex and functions as a repressor of SL signalling
- D3, D53, DWARF 53 acts as a repressor of strigolactone signalling in rice, Treatments with GR24, a synthetic SL analogue, cause D53 degradation via the proteasome in a manner that requires D14 and the SCF(D3) ubiquitin ligase, whereas the dominant form of D53 is resistant to SL-mediated degradation
- D3, D53, DWARF 53 acts as a repressor of strigolactone signalling in rice, Our results suggest a model of SL signalling that involves SL-dependent degradation of the D53 repressor mediated by the D14-D3 complex
- D53, HTD2~D88~D14, DWARF 53 acts as a repressor of strigolactone signalling in rice, Treatments with GR24, a synthetic SL analogue, cause D53 degradation via the proteasome in a manner that requires D14 and the SCF(D3) ubiquitin ligase, whereas the dominant form of D53 is resistant to SL-mediated degradation
- D53, HTD2~D88~D14, DWARF 53 acts as a repressor of strigolactone signalling in rice, Our results suggest a model of SL signalling that involves SL-dependent degradation of the D53 repressor mediated by the D14-D3 complex
- D3, D53, D14-SCFD3-dependent degradation of D53 regulates strigolactone signalling, The D53 gene product shares predicted features with the class I Clp ATPase proteins and can form a complex with the alpha/beta hydrolase protein DWARF 14 (D14) and the F-box protein DWARF 3 (D3), two previously identified signalling components potentially responsible for SL perception
- D3, D53, D14-SCFD3-dependent degradation of D53 regulates strigolactone signalling, We demonstrate that, in a D14- and D3-dependent manner, SLs induce D53 degradation by the proteasome and abrogate its activity in promoting axillary bud outgrowth
- D3, D53, D14-SCFD3-dependent degradation of D53 regulates strigolactone signalling, D14-SCF(D3)-dependent degradation of D53 regulates strigolactone signalling
- D53, HTD2~D88~D14, D14-SCFD3-dependent degradation of D53 regulates strigolactone signalling, The D53 gene product shares predicted features with the class I Clp ATPase proteins and can form a complex with the alpha/beta hydrolase protein DWARF 14 (D14) and the F-box protein DWARF 3 (D3), two previously identified signalling components potentially responsible for SL perception
- D53, HTD2~D88~D14, D14-SCFD3-dependent degradation of D53 regulates strigolactone signalling, We demonstrate that, in a D14- and D3-dependent manner, SLs induce D53 degradation by the proteasome and abrogate its activity in promoting axillary bud outgrowth
- D53, HTD2~D88~D14, D14-SCFD3-dependent degradation of D53 regulates strigolactone signalling, D14-SCF(D3)-dependent degradation of D53 regulates strigolactone signalling
- D53, HTD2~D88~D14, Identification and Molecular Mapping of Indica High-tillering Dwarf Mutant htd4, a Mild Phenotype Allelic Mutant of D14 in Rice Oryza sativa L.., Quantitative RT-PCR analyses revealed that expression levels of the genes D10, D17, D27, D3, and D14 increased significantly while expression of D53 decreased in htd4, compared to the wild type
- D53, OsSPL14~IPA1~WFP, IPA1 functions as a downstream transcription factor repressed by D53 in strigolactone signaling in rice., IPA1 functions as a downstream transcription factor repressed by D53 in strigolactone signaling in rice.
- D53, OsSPL14~IPA1~WFP, IPA1 functions as a downstream transcription factor repressed by D53 in strigolactone signaling in rice., Here we report that Ideal Plant Architecture 1 (IPA1), a key regulator of the plant architecture in rice, functions as a direct downstream component of D53 in regulating tiller number and SL-induced gene expression
- D53, OsSPL14~IPA1~WFP, IPA1 functions as a downstream transcription factor repressed by D53 in strigolactone signaling in rice., We showed that D53 interacts with IPA1 in vivo and in vitro and suppresses the transcriptional activation activity of IPA1
- D53, OsSPL14~IPA1~WFP, IPA1 functions as a downstream transcription factor repressed by D53 in strigolactone signaling in rice., We further showed that IPA1 could directly bind to the D53 promoter and plays a critical role in the feedback regulation of SL-induced D53 expression
- D53, OsSPL14~IPA1~WFP, IPA1 functions as a downstream transcription factor repressed by D53 in strigolactone signaling in rice., These findings reveal that IPA1 is likely one of the long-speculated transcription factors that act with D53 to mediate the SL-regulated tiller development in rice
- D53, HTD2~D88~D14, DWARF14, A Receptor Covalently Linked with the Active Form of Strigolactones, Undergoes Strigolactone-Dependent Degradation in Rice., This stimulates the interaction between D14 and D3, leading to the ubiquitination and degradation of the transcriptional repressor protein D53
- D53, HTD2~D88~D14, DWARF14, A Receptor Covalently Linked with the Active Form of Strigolactones, Undergoes Strigolactone-Dependent Degradation in Rice., The Lys280 site of the D14 amino acid sequence was important for SL-induced D14 degradation, but did not change the subcellular localization of D14 nor disturbed the interaction between D14 and D3, nor D53 degradation
- D53, HTD2~D88~D14, DWARF14, A Receptor Covalently Linked with the Active Form of Strigolactones, Undergoes Strigolactone-Dependent Degradation in Rice., We further showed that D14 degradation is dependent on D3 and is tightly correlated with protein levels of D53
- D53, HTD2~D88~D14, DWARF14, A Receptor Covalently Linked with the Active Form of Strigolactones, Undergoes Strigolactone-Dependent Degradation in Rice., These findings revealed that D14 degradation takes place following D53 degradation and functions as an important feedback regulation mechanism of SL perception in rice
- D53, HTD2~D88~D14, Rationally Designed Strigolactone Analogs as Antagonists of the D14 Receptor., This cleavage reaction (hydrolysis and dissociation) is important for inducing the interaction between D14 and its target proteins, including D3 and D53
- D53, HTD2~D88~D14, Rationally Designed Strigolactone Analogs as Antagonists of the D14 Receptor., Subsequent biochemical and physiological studies indicated that carba-SLs blocked the interaction between D14 and D53 by inhibiting D14 hydrolytic activity
- D53, OsCKX9, Strigolactone promotes cytokinin degradation through transcriptional activation of CYTOKININ OXIDASE/DEHYDROGENASE 9 in rice., Here we show that the cytokinin (CK) content is dramatically increased in shoot bases of the rice SL signaling mutant d53 By examining transcript levels of all the CK metabolism-related genes after treatment with SL analog GR24, we identified CYTOKININ OXIDASE/DEHYDROGENASE 9 (OsCKX9) as a primary response gene significantly up-regulated within 1 h of treatment in the wild type but not in d53 We also found that OsCKX9 functions as a cytosolic and nuclear dual-localized CK catabolic enzyme, and that the overexpression of OsCKX9 suppresses the browning of d53 calli
- D53, OsBZR1~BZR1, Strigolactones and brassinosteroids antagonistically regulate the stability of D53-OsBZR1 complex to determine FC1 expression in rice tillering., Further experiments demonstrate that D53 interacts with OsBZR1 to inhibit FC1 expression, the local inhibitor for tillering, which depends on the direct DNA-binding by OsBZR1 to recruit D53 to FC1 promoter in rice buds
- D53, OsSMAX1, Karrikin Signaling Acts Parallel to and Additively with Strigolactone Signaling to Regulate Rice Mesocotyl Elongation in Darkness , We demonstrate that D14L recognizes the karrikin signal and recruits the SCFD3 ubiquitin ligase for the ubiquitination and degradation of OsSMAX1, mirroring the SL-induced and D14- and D3-dependent ubiquitination and degradation of D53
- D3, D53, Sucrose promotes D53 accumulation and tillering in rice., Overexpression of D3 antagonizes sucrose inhibition of D53 degradation and enables the SL inhibition of tillering under high sucrose
- D3, D53, Sucrose promotes D53 accumulation and tillering in rice., In contrast to D3, D14 overexpression enhances D53 protein levels and sucrose-induced tillering, even in the presence of SL
- D53, HTD2~D88~D14, Sucrose promotes D53 accumulation and tillering in rice., Sucrose prevents SL-induced degradation of D14, the SL receptor involved in D53 degradation
- D53, HTD2~D88~D14, Sucrose promotes D53 accumulation and tillering in rice., In contrast to D3, D14 overexpression enhances D53 protein levels and sucrose-induced tillering, even in the presence of SL
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