ASR5,OsAsr1,OsASR2

2015-01-20 | Categories genes  | Tags vegetative  root  palea  growth  transcription factor  photosynthesis  temperature  reproductive  shoot  aluminum  drought  sheath  gibberellin  cold stress  cold tolerance  lemma  abiotic stress  leaf  chloroplast  seedlings  resistance  stress  biotic stress 

• Information
  • Symbol: ASR5,OsAsr1,OsASR2
  • MSU: LOC_Os11g06720
  • RAPdb: Os11g0167800
• Publication
  • Structure, allelic diversity and selection of Asr genes, candidate for drought tolerance, in Oryza sativa L. and wild relatives, 2010, Theor Appl Genet.
  • Identification and characterization of a gibberellin-regulated protein, which is ASR5, in the basal region of rice leaf sheaths, 2008, Mol Genet Genomics.
  • Ectopic expression of a cold-responsive OsAsr1 cDNA gives enhanced cold tolerance in transgenic rice plants, 2009, Mol Cells.
  • Involvement of ASR genes in aluminium tolerance mechanisms in rice, 2013, Plant Cell Environ.
  • The rice ASR5 protein: a putative role in the response to aluminum photosynthesis disturbance, 2012, Plant Signal Behav.
  • Abiotic stress responsive rice ASR1 and ASR3 exhibit different tissue-dependent sugar and hormone-sensitivities, 2013, Mol Cells.
  • Rice ASR1 protein with reactive oxygen species scavenging and chaperone-like activities enhances acquired tolerance to abiotic stresses in Saccharomyces cerevisiae, 2012, Mol Cells.
  • ASR5 is involved in the regulation of miRNA expression in rice., 2015, Plant Cell Rep.
  • OsASR2 regulates the expression of a defense-related gene, Os2H16, by targeting the GT-1 cis-element., 2017, Plant Biotechnol J.
• Genbank accession number
  • AAB96681
  • EF576160
  • AAB96681
  • EF576160
• Key message
  • Low-temperature stress increased mRNA levels of OsAsr1 in both vegetative and reproductive organs
  • Recently, we focused on ASR (Abscisic acid, Stress and Ripening) gene expression analyses and observed increased ASR5 transcript levels in roots and shoots in response to Al
  • In situ analysis showed that OsAsr1 transcript was preferentially accumulated in the leaf mesophyll tissues and parenchyma cells of the palea and lemma
  • Since this protein is regulated not only by abscisic acid but also by GA(3), these results indicate that ASR5 might be involved in plant growth in addition to stress in the basal regions of leaf sheaths
  • Because it is well known that Al induces photosynthetic dysfunction, here we discuss the hypothesis that ASR5 might be sequestered in the chloroplasts as an inactive transcription factor that could be released to the nucleus in response to Al to regulate genes related to photosynthesis
  • A proteomic approach showed that ASR5 silencing affected several proteins related to photosynthesis in RNAi rice shoots
  • The rice ASR5 protein: a putative role in the response to aluminum photosynthesis disturbance
  • Taken together, we suggest that OsAsr1 gene play an important role during temperature stress, and that this gene can be used for generating plants with enhanced cold tolerance
  • The expression of both OsASR1 and OsASR3 was induced by drought stress, which is a major regulator of the expression of all ASR genes in rice
  • Our data also showed that the overexpression of either OsASR1 or OsASR3 in transgenic rice plants increased their tolerance to drought and cold stress
  • ASR5 out of these six proteins was significantly regulated by GA(3) at the protein level but not at the mRNA level in the basal region of leaf sheaths
  • Identification and characterization of a gibberellin-regulated protein, which is ASR5, in the basal region of rice leaf sheaths
  • We suggest that the ASR5 protein acts as a transcription factor to regulate the expression of different genes that collectively protect rice cells from Al-induced stress responses
  • Ectopic expression of a cold-responsive OsAsr1 cDNA gives enhanced cold tolerance in transgenic rice plants
  • OsASR1 and OsASR3 are the most abundant and are found in most tissues; they are enriched in the leaves and roots, respectively
  • , OsASR1 in leaves and OsASR3 in roots)
  • Coexpression analysis of OsASR1 and OsASR3 and a comparison of the cis-acting elements upstream of OsASR1 and OsASR3 suggested that their expression is regulated in common by abiotic stresses but differently regulated by hormone and sugar signals
  • Furthermore, an ASR5-GFP fusion in rice protoplasts revealed for the first time a chloroplast localization of this protein
  • This work describes the transcriptomic analysis by deep sequencing two libraries, comparing miRNA abundance from the roots of transgenic ASR5 knockdown rice seedlings with that of the roots of wild-type non-transformed rice seedlings
  • Overexpression of OsASR2 enhanced the resistance against Xanthomonas oryzae pv
  • These results suggest that the interaction between OsASR2 and GT-1 plays an important role in the crosstalk of the response of rice to biotic and abiotic stresses
  • Additionally, the expression of OsASR2 was elevated by pathogens and osmotic stress challenges
• Connection
  • Asr3~OsASR3, ASR5~OsAsr1~OsASR2, Abiotic stress responsive rice ASR1 and ASR3 exhibit different tissue-dependent sugar and hormone-sensitivities, OsASR1 and OsASR3 are the most abundant and are found in most tissues; they are enriched in the leaves and roots, respectively
  • Asr3~OsASR3, ASR5~OsAsr1~OsASR2, Abiotic stress responsive rice ASR1 and ASR3 exhibit different tissue-dependent sugar and hormone-sensitivities, Coexpression analysis of OsASR1 and OsASR3 and a comparison of the cis-acting elements upstream of OsASR1 and OsASR3 suggested that their expression is regulated in common by abiotic stresses but differently regulated by hormone and sugar signals
  • Asr3~OsASR3, ASR5~OsAsr1~OsASR2, Abiotic stress responsive rice ASR1 and ASR3 exhibit different tissue-dependent sugar and hormone-sensitivities, The results of quantitative real-time PCR analyses of OsASR1 and OsASR3 expression under various conditions further support this model
  • Asr3~OsASR3, ASR5~OsAsr1~OsASR2, Abiotic stress responsive rice ASR1 and ASR3 exhibit different tissue-dependent sugar and hormone-sensitivities, The expression of both OsASR1 and OsASR3 was induced by drought stress, which is a major regulator of the expression of all ASR genes in rice
  • Asr3~OsASR3, ASR5~OsAsr1~OsASR2, Abiotic stress responsive rice ASR1 and ASR3 exhibit different tissue-dependent sugar and hormone-sensitivities, OsASR1 transcription was highly induced by ABA, whereas OsASR3 transcription was strongly induced by GA
  • Asr3~OsASR3, ASR5~OsAsr1~OsASR2, Abiotic stress responsive rice ASR1 and ASR3 exhibit different tissue-dependent sugar and hormone-sensitivities, In addition, OsASR1 and OsASR3 expression was significantly induced by sucrose and sucrose/glucose treatments, respectively
  • Asr3~OsASR3, ASR5~OsAsr1~OsASR2, Abiotic stress responsive rice ASR1 and ASR3 exhibit different tissue-dependent sugar and hormone-sensitivities, Our data also showed that the overexpression of either OsASR1 or OsASR3 in transgenic rice plants increased their tolerance to drought and cold stress
  • Asr3~OsASR3, ASR5~OsAsr1~OsASR2, Abiotic stress responsive rice ASR1 and ASR3 exhibit different tissue-dependent sugar and hormone-sensitivities, Abiotic stress responsive rice ASR1 and ASR3 exhibit different tissue-dependent sugar and hormone-sensitivities
  • ASR5~OsAsr1~OsASR2, Os2H16, OsASR2 regulates the expression of a defense-related gene, Os2H16, by targeting the GT-1 cis-element., OsASR2 regulates the expression of a defense-related gene, Os2H16, by targeting the GT-1 cis-element.
  • ASR5~OsAsr1~OsASR2, Os2H16, OsASR2 regulates the expression of a defense-related gene, Os2H16, by targeting the GT-1 cis-element., Furthermore, we identified that OsASR2 was specifically bound to GT-1 and activated the expression of the target gene Os2H16, as well as GFP driven by the chimeric promoter of 2 × GT-1-35S mini construct
  • ASR5~OsAsr1~OsASR2, OsSKL2, Regulation of Drought and Salt Tolerance by OsSKL2 and OsASR1 in Rice., Regulation of Drought and Salt Tolerance by OsSKL2 and OsASR1 in Rice.
  • ASR5~OsAsr1~OsASR2, OsSKL2, Regulation of Drought and Salt Tolerance by OsSKL2 and OsASR1 in Rice., Moreover, OsSKL2 was found to physically interact with OsASR1, a well-known chaperone-like protein, which also exhibited positive roles in salt and drought tolerance
  • ASR5~OsAsr1~OsASR2, OsSKL2, Regulation of Drought and Salt Tolerance by OsSKL2 and OsASR1 in Rice., A reduction in ROS production was also observed in leaves of Nicotiana benthamiana showing transient co-expression of OsSKL2 with OsASR1
  • ASR5~OsAsr1~OsASR2, OsSKL2, Regulation of Drought and Salt Tolerance by OsSKL2 and OsASR1 in Rice., Taken together, these findings suggest that OsSKL2 together with OsASR1 act as important regulatory factors that confer salt and drought tolerance in rice via ROS scavenging

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