- Information
- Symbol: OsNAC5
- MSU: LOC_Os11g08210
- RAPdb: Os11g0184900
- Publication
- Physiological mechanisms underlying OsNAC5-dependent tolerance of rice plants to abiotic stress, 2011, Planta.
- The abiotic stress-responsive NAC-type transcription factor OsNAC5 regulates stress-inducible genes and stress tolerance in rice, 2010, Mol Genet Genomics.
- OsNAC5 overexpression enlarges root diameter in rice plants leading to enhanced drought tolerance and increased grain yield in the field, 2013, Plant Biotechnol J.
- Molecular analysis of the NAC gene family in rice, 2000, Mol Gen Genet.
- Identification of up-regulated genes in flag leaves during rice grain filling and characterization of OsNAC5, a new ABA-dependent transcription factor, 2009, Planta.
- Transcriptional activation of rice CINNAMOYL-CoA REDUCTASE 10 by OsNAC5, contributes to drought tolerance by modulating lignin accumulation in roots., 2021, Plant Biotechnol J.
- Enhanced expression of OsNAC5 leads to up-regulation of OsNAC6 and changes rice Oryza sativa L. ionome., 2023, Genet Mol Biol.
- Genbank accession number
- Key message
- To understand the functions of transcription factor OsNAC5 in response to abiotic stress, we generated transgenic rice plants with knockdown OsNAC5 by RNA-interfered (RNAi) and overexpressing OsNAC5, and investigated the effects of cold, drought and salt stress on wild-type (WT), RNAi and overexpression rice lines
- These findings highlight the important role of OsNAC5 played in the tolerance of rice plants to abiotic stress by regulating downstream targets associated with accumulation of compatible solutes, Na(+) ions, H(2)O(2) and malondialdehyde
- Physiological mechanisms underlying OsNAC5-dependent tolerance of rice plants to abiotic stress
- Both the RCc3:OsNAC5 and GOS2:OsNAC5 plants were found to have larger roots due to an enlarged stele and aerenchyma at flowering stage
- Field evaluations over three growing seasons revealed that the grain yield of the RCc3:OsNAC5 and GOS2:OsNAC5 plants were increased by 9%-23% and 9%-26% under normal conditions, respectively
- Under drought conditions, however, RCc3:OsNAC5 plants showed a significantly higher grain yield of 22%-63%, whilst the GOS2:OsNAC5 plants showed a reduced or similar yield to the nontransgenic (NT) controls
- Our present findings demonstrate that the root-specific overexpression of OsNAC5 enlarges roots significantly and thereby enhances drought tolerance and grain yield under field conditions
- OsNAC5 overexpression enlarges root diameter in rice plants leading to enhanced drought tolerance and increased grain yield in the field
- The root diameter was enlarged to a greater extent in the RCc3:OsNAC5, suggesting the importance of this phenotype for enhanced drought tolerance
- Of the genes specifically up-regulated in the RCc3:OsNAC5 roots, GLP, PDX, MERI5 and O-methyltransferase were implicated in root growth and development
- Expression of OsNAC5 is induced by abiotic stresses such as drought, cold, high salinity, abscisic acid and methyl jasmonic acid
- The abiotic stress-responsive NAC-type transcription factor OsNAC5 regulates stress-inducible genes and stress tolerance in rice
- We show that OsNAC5 expression is up-regulated by natural (aging) and induced senescence processes (dark, ABA application, high salinity, cold and Fe-deficiency) and its expression is not affected in the presence of 6-benzylaminopurine (a senescence inhibitor) under dark-induced senescence
- Using four different rice cultivars, we show that OsNAC5 up-regulation is higher and earlier in flag leaves and panicles of IR75862 plants, which have higher seed concentrations of Fe, Zn and protein
- We suggest that OsNAC5 is a novel senescence-associated ABA-dependent NAC transcription factor and its function could be related to Fe, Zn and amino acids remobilization from green tissues to seeds
- The transcription factor OsNAC5 in rice is a member of the plant-specific NAC family that regulates stress responses
- OsNAC5-overexpressing transgenic plants also had improved tolerance to high salinity compared to control plants
- Salt induction of OsNAC5 expression is abolished by nicotinamide, an inhibitor of ABA effects
- Collectively, our results indicate that the stress-responsive proteins OsNAC5 and OsNAC6 are transcriptional activators that enhance stress tolerance by upregulating the expression of stress-inducible rice genes such as OsLEA3, although the effects of these proteins on growth are different
- Furthermore, because OsNAC5 overexpression did not retard growth, OsNAC5 may be a useful gene that can improve the stress tolerance of rice without affecting its growth
- Differential expression of selected genes (encoding 7 transport proteins, the OsNAS3 enzyme and the OsNAC5 transcription factor) was confirmed by quantitative RT-PCR
- Identification of up-regulated genes in flag leaves during rice grain filling and characterization of OsNAC5, a new ABA-dependent transcription factor
- In addition, knockdown and overexpression of OsNAC5 enhanced and reduced accumulation of malondialdehyde and H(2)O(2), suggesting that knockdown of OsNAC5 renders RNAi plants more susceptible to oxidative damage
- Here, we report the results of field evaluations of transgenic rice plants overexpressing OsNAC5, under the control of either the root-specific (RCc3) or constitutive (GOS2) promoters
- Also identified were 19 and 18 up-regulated genes that are specific to the RCc3:OsNAC5 and GOS2:OsNAC5 roots, respectively
- Here, we report that the rice (Oryza sativa) gene CINNAMOYL-CoA REDUCTASE 10 (OsCCR10) is directly activated by the OsNAC5 transcription factor, which mediates drought tolerance through regulating lignin accumulation
- Aiming a better understanding of the role of OsNAC5 in rice plants, we investigated a mutant line carrying a T-DNA insertion in the promoter of OsNAC5, which resulted in enhanced expression of the transcription factor
- Plants with OsNAC5 enhanced expression were shorter at the seedling stage and had reduced yield at maturity
- Ionomic analysis of leaves and seeds from the OsNAC5 enhanced expression line revealed lower Fe and Zn concentrations in leaves and higher Fe concentrations in seeds than in WT plants, further suggesting that OsNAC5 may be involved in regulating the ionome in rice plants
- Connection
- OsLEA3~OsLEA3-1, OsNAC5, The abiotic stress-responsive NAC-type transcription factor OsNAC5 regulates stress-inducible genes and stress tolerance in rice, By microarray analysis, many stress-inducible genes, including the “late embryogenesis abundant” gene OsLEA3, were upregulated in rice plants that overexpressed OsNAC5
- OsLEA3~OsLEA3-1, OsNAC5, The abiotic stress-responsive NAC-type transcription factor OsNAC5 regulates stress-inducible genes and stress tolerance in rice, By gel mobility shift assay, OsNAC5 and OsNAC6 were shown to bind to the OsLEA3 promoter
- OsLEA3~OsLEA3-1, OsNAC5, The abiotic stress-responsive NAC-type transcription factor OsNAC5 regulates stress-inducible genes and stress tolerance in rice, Collectively, our results indicate that the stress-responsive proteins OsNAC5 and OsNAC6 are transcriptional activators that enhance stress tolerance by upregulating the expression of stress-inducible rice genes such as OsLEA3, although the effects of these proteins on growth are different
- OsNAC19~SNAC1~OsNAC9, OsNAC5, The abiotic stress-responsive NAC-type transcription factor OsNAC5 regulates stress-inducible genes and stress tolerance in rice, Pull-down assays revealed that OsNAC5 interacts with OsNAC5, OsNAC6 and SNAC1
- OsNAC5, OsNAC6~SNAC2, The abiotic stress-responsive NAC-type transcription factor OsNAC5 regulates stress-inducible genes and stress tolerance in rice, Pull-down assays revealed that OsNAC5 interacts with OsNAC5, OsNAC6 and SNAC1
- OsNAC5, OsNAC6~SNAC2, The abiotic stress-responsive NAC-type transcription factor OsNAC5 regulates stress-inducible genes and stress tolerance in rice, By gel mobility shift assay, OsNAC5 and OsNAC6 were shown to bind to the OsLEA3 promoter
- OsNAC5, OsNAC6~SNAC2, The abiotic stress-responsive NAC-type transcription factor OsNAC5 regulates stress-inducible genes and stress tolerance in rice, Collectively, our results indicate that the stress-responsive proteins OsNAC5 and OsNAC6 are transcriptional activators that enhance stress tolerance by upregulating the expression of stress-inducible rice genes such as OsLEA3, although the effects of these proteins on growth are different
- OsNAC5, OsNAS3, Identification of up-regulated genes in flag leaves during rice grain filling and characterization of OsNAC5, a new ABA-dependent transcription factor, Differential expression of selected genes (encoding 7 transport proteins, the OsNAS3 enzyme and the OsNAC5 transcription factor) was confirmed by quantitative RT-PCR
- ONAC106, OsNAC5, Rice ONAC106 inhibits leaf senescence and increases salt tolerance and tiller angle., Using yeast one-hybrid assays, we found that ONAC106 binds to the promoter regions of SGR, NYC1, OsNAC5, and LPA1
- OsCCR10, OsNAC5, Transcriptional activation of rice CINNAMOYL-CoA REDUCTASE 10 by OsNAC5, contributes to drought tolerance by modulating lignin accumulation in roots., Here, we report that the rice (Oryza sativa) gene CINNAMOYL-CoA REDUCTASE 10 (OsCCR10) is directly activated by the OsNAC5 transcription factor, which mediates drought tolerance through regulating lignin accumulation
- OsNAC5, OsNAC6~SNAC2, Enhanced expression of OsNAC5 leads to up-regulation of OsNAC6 and changes rice Oryza sativa L. ionome., Enhanced expression of OsNAC5 leads to up-regulation of OsNAC6 and changes rice (Oryza sativa L.) ionome.
- OsNAC5, OsNAC6~SNAC2, Enhanced expression of OsNAC5 leads to up-regulation of OsNAC6 and changes rice Oryza sativa L. ionome., In addition, we evaluated the expression level of OsNAC6, which is co-expressed with OsNAC5, and found that enhanced expression of OsNAC5 leads to increased expression of OsNAC6, suggesting that OsNAC5 might regulate OsNAC6 expression
- OsNAC5, ZN, Enhanced expression of OsNAC5 leads to up-regulation of OsNAC6 and changes rice Oryza sativa L. ionome., Ionomic analysis of leaves and seeds from the OsNAC5 enhanced expression line revealed lower Fe and Zn concentrations in leaves and higher Fe concentrations in seeds than in WT plants, further suggesting that OsNAC5 may be involved in regulating the ionome in rice plants
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