- Information
- Symbol: OsCRTISO,ZEBRA2,MHZ5,MIT3
- MSU: LOC_Os11g36440
- RAPdb: Os11g0572700
- Publication
- ZEBRA2, encoding a carotenoid isomerase, is involved in photoprotection in rice, 2011, Plant Mol Biol.
- Mutations of genes in synthesis of the carotenoid precursors of ABA lead to pre-harvest sprouting and photo-oxidation in rice, 2008, Plant J.
- Ethylene Responses in Rice Roots and Coleoptiles Are Differentially Regulated by a Carotenoid Isomerase-Mediated Abscisic Acid Pathway., 2015, Plant Cell.
- Mutations in the MIT3 gene encoding a caroteniod isomerase lead to increased tiller number in rice., 2017, Plant Sci.
- Genbank accession number
- Key message
- The greatly increased activities of reactive oxygen species (ROS) scavenging enzymes, and reduced photosystem (PS) II core proteins CP43, CP47 and D1 in leaves of the Oscrtiso/phs3-1mutant and OsLCY RNAi transgenic rice indicated that photo-oxidative damage occurred in PS II, consistent with the accumulation of ROS in these plants
- Expression analysis via both RT-PCR and ZEBRA2-promoter-beta-glucuronidase (GUS) transgenic rice indicates that ZEBRA2 is predominantly expressed in mesophyll cells of mature leaves where active photosynthesis occurs
- More severe phenotype of the zebra2 mutant under high light intensity indicates that ““zebra”” phenotype might be caused by photooxidative damages
- We found that MHZ5 encodes a carotenoid isomerase and that the mutation in mhz5 blocks carotenoid biosynthesis, reduces ABA accumulation, and promotes ethylene production in etiolated seedlings
- ABA can largely rescue the ethylene response of the mhz5 mutant
- Ethylene induces MHZ5 expression, the production of neoxanthin, an ABA biosynthesis precursor, and ABA accumulation in roots
- MHZ5 overexpression results in enhanced ethylene sensitivity in roots and reduced ethylene sensitivity in coleoptiles
- Prolycopene was accumulated while all-trans-lycopene was barely detectable in the dark-grown mit3 seedlings
- Exogenously applied GR24, a synthetic SL, could rescue the tillering phenotype of mit3
- Our results reveal that the tillering phenotype of mit3 is due to SL deficiency and directly link carotenoid deficiency with SL-regulated rice tillering
- Mutations in the MIT3 gene encoding a caroteniod isomerase lead to increased tiller number in rice.
- Double mutant analysis of mit3 with the SLs biosynthesis mutant d17 revealed that MIT3 controls tiller development upstream of the SLs biosynthesis pathway
- Map-based cloning revealed that MIT3 encodes a carotenoid isomerase (CRTISO), the key enzyme catalyzing the conversion from prolycopene to all-trans-lycopene in carotenoid biosynthesis
- Connection
- OsCRTISO~ZEBRA2~MHZ5~MIT3, OsPDS, Mutations of genes in synthesis of the carotenoid precursors of ABA lead to pre-harvest sprouting and photo-oxidation in rice, Here we characterize in detail one of these groups, which contains mutations in genes encoding major enzymes of the carotenoid biosynthesis pathway, including phytoene desaturase (OsPDS), zeta-carotene desaturase (OsZDS), carotenoid isomerase (OsCRTISO) and lycopene beta-cyclase (beta-OsLCY), which are essential for the biosynthesis of carotenoid precursors of ABA
- OsCRTISO~ZEBRA2~MHZ5~MIT3, OsZDS, Mutations of genes in synthesis of the carotenoid precursors of ABA lead to pre-harvest sprouting and photo-oxidation in rice, Here we characterize in detail one of these groups, which contains mutations in genes encoding major enzymes of the carotenoid biosynthesis pathway, including phytoene desaturase (OsPDS), zeta-carotene desaturase (OsZDS), carotenoid isomerase (OsCRTISO) and lycopene beta-cyclase (beta-OsLCY), which are essential for the biosynthesis of carotenoid precursors of ABA
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