OsFAD3

2015-01-20 | Categories genes  | Tags growth  seed  chilling  leaf  temperature  root 

• Information
  • Symbol: OsFAD3
  • MSU: LOC_Os12g01370
  • RAPdb: Os12g0104400
• Publication
  • Structure, chromosomal location and expression of a rice gene encoding the microsome omega-3 fatty acid desaturase, 1997, Plant Mol Biol.
  • Identification and evaluation of omega-3 fatty acid desaturase genes for hyperfortifying alpha-linolenic acid in transgenic rice seed, 2012, J Exp Bot.
• Genbank accession number
  • AK071185
• Key message
  • The accumulation of the OsFAD3 mRNA in leaf tissues remained at quite low levels, both at normal growth temperatures and at chilling temperatures
  • The ALA contents in the seeds of endoplasmic reticulum (ER)-localized GmFAD3-1 and OsFAD3 overexpression lines increased from 0
  • Homologous OsFAD3 is more active than GmFAD3-1 in catalysing LA conversion to ALA in rice seeds
  • Similar temperature responses of the OsFAD3 gene were observed both in chilling- tolerant and in chilling-intolerant rice cultivars
  • In root tissues, a high level of the OsFAD3 mRNA was observed at 15 degrees C and 20 degrees C, with its level decreasing markedly at temperatures below 10 degrees C
  • Expression of the OsFAD3 cDNA in tobacco hairy root tissues and subsequent analysis of fatty acid compositions demonstrated the activity of the microsome omega-3 fatty acid desaturase
  • The OsFAD3 mRNA was abundant in root tissues, but was hardly detectable in leaves
• Connection
  • OsFAD3, OsFAD7, Identification and evaluation of omega-3 fatty acid desaturase genes for hyperfortifying alpha-linolenic acid in transgenic rice seed, Overexpression of ER-localized GmFAD3-2/3 and chloroplast-localized OsFAD7/8 had less effect on increasing the ALA content in rice seeds

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