OsMYB60

• Symbol: OsMYB60
• MSU: LOC_Os12g03150
• RAPdb: Os12g0125000

• A novel antisense long noncoding RNA, TWISTED LEAF, maintains leaf blade flattening by regulating its associated sense R2R3-MYB gene in rice., 2018, New Phytol.
• Mutation of OsMYB60 reduces rice resilience to drought stress by attenuating cuticular wax biosynthesis., 2022, Plant J.

Genbank accession number

• Downregulation of TL by RNA interference (RNAi) and overexpression of OsMYB60 resulted in twisted leaf blades in transgenic rice
• This suggests that TL may play a cis-regulatory role on OsMYB60 in leaf morphological development
• We further discovered that a C2H2 transcription factor, OsZFP7, is an OsMYB60 binding partner and involved in leaf development
• Quantitative reverse-transcription PCR showed that the loss-of-function of OsMYB60 led to downregulation of wax biosynthetic genes, leading to reduced amounts of total wax components on leaf surfaces under normal conditions
• Taken together, these results demonstrate that OsMYB60 contributes to enhancing rice resilience to drought stress by promoting cuticular wax biosynthesis on leaf surfaces
• Here, we show that the MYB transcription factor OsMYB60 positively regulates cuticular wax biosynthesis and this helps rice (Oryza sativa) plants tolerate drought stress
• Mutation of OsMYB60 reduces rice resilience to drought stress by attenuating cuticular wax biosynthesis.
• Compared with the wild type (japonica cultivar 'Dongjin'), osmyb60 null mutants (osmyb60-1 and osmyb60-2) exhibited increased drought sensitivity, with higher chlorophyll leaching and faster rates of water loss

• OsCER1~OsGL1-4~LTR1, OsMYB60, Mutation of OsMYB60 reduces rice resilience to drought stress by attenuating cuticular wax biosynthesis., Yeast one-hybrid, luciferase transient transcriptional activity, and chromatin immunoprecipitation assays revealed that OsMYB60 directly binds to the promoter of OsCER1 (a key gene involved in very-long-chain alkane biosynthesis) and upregulates its expression