- Information
- Symbol: OSIPK,OsCPK29
- MSU: LOC_Os12g12860
- RAPdb: Os12g0230200
- Publication
- Genbank accession number
- Key message
- Differential screening of a stage-specific cDNA library of Indica rice has been used to identify two genes expressed in pre-pollination stage panicles, namely OSIPA and OSIPK coding for proteins similar to expansins/pollen allergens and calcium-dependent protein kinases (CDPK), respectively
- Northern analysis and in situ hybridizations indicate that OSIPA expresses exclusively in pollen while OSIPK expresses in pollen as well as anther wall
- Promoter of OSIPA is active during the late stages of pollen development and remains active till the anthesis, whereas, OSIPK promoter is active to a low level in developing anther till the pollen matures
- Furthermore, it has been demonstrated that OsCPK29 interacts in vitro as well as in vivo with the MADS68 transcription factor which is a known regulator of pollen development
- OsCPK29 interacts with MADS68 to regulate pollen development in rice.
- Therefore, phenotypic observations and molecular studies suggest that OsCPK29 is an important regulator of pollen development in rice
- Here, we report the functional characterization of a novel OsCPK29 from rice, which is mainly expressed during pollen maturation stages of the anther
- OsCPK29 knockdown rice plants exhibit reduced fertility, set fewer seeds, and produce collapsed non-viable pollen grains that do not germinate
- OsCPK29 exclusively localizes in the nucleus, and its N-terminal variable domain is responsible for retaining it in the nucleus
- Connection
- OSIPA, OSIPK~OsCPK29, Promoters of two anther-specific genes confer organ-specific gene expression in a stage-specific manner in transgenic systems, Differential screening of a stage-specific cDNA library of Indica rice has been used to identify two genes expressed in pre-pollination stage panicles, namely OSIPA and OSIPK coding for proteins similar to expansins/pollen allergens and calcium-dependent protein kinases (CDPK), respectively
- OSIPA, OSIPK~OsCPK29, Promoters of two anther-specific genes confer organ-specific gene expression in a stage-specific manner in transgenic systems, Northern analysis and in situ hybridizations indicate that OSIPA expresses exclusively in pollen while OSIPK expresses in pollen as well as anther wall
- OSIPA, OSIPK~OsCPK29, Promoters of two anther-specific genes confer organ-specific gene expression in a stage-specific manner in transgenic systems, Promoter of OSIPA is active during the late stages of pollen development and remains active till the anthesis, whereas, OSIPK promoter is active to a low level in developing anther till the pollen matures
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