Adk-a,OsAK3

2015-01-20 | Categories genes  | Tags temperature  growth  adenylate kinase  development  Kinase  spikelet  grain  grain length  grain size  root  brassinosteroid  BR  Brassinosteroid  BR signaling  lamina  kinase  Brassinosteroid Signaling 

• Information
  • Symbol: Adk-a,OsAK3
  • MSU: LOC_Os12g13380
  • RAPdb: Os12g0236400
• Publication
  • Molecular characterization of cDNA encoding for adenylate kinase of rice Oryza sativa L., 1992, Plant J.
  • An Adenylate Kinase OsAK3 Involves Brassinosteroid Signaling and Grain Length in Rice Oryza sativa L.., 2021, Rice (N Y).
• Genbank accession number
  • D10334
• Key message
  • In order to examine the enzyme activity of the protein encoded by the rice cDNA, Adk-a was cloned into an expression vector, pUC119, which was introduced into Escherichia coli strain CV2, a temperature-sensitive mutant of adenylate kinase
  • We found that the transformant carrying the rice Adk-a gene in the sense orientation recovered cell growth at non-permissive high temperature (42 degrees C) and expressed enzyme activities higher than the untransformed CV2 and the transformant possessing Adk-a cDNA in the antisense orientation
  • Two types of genes (Adk-a, and Adk-b) encoding for adenylate kinase (AK, EC 2.7.4.3.) were isolated from the cDNA library constructed from poly(A)+ RNA of rice (Oryza sativa L.).
  • CONCLUSIONS: Our study reveals a novel BR signaling component OsAK3 in the regulation of grain length, and provides novel clues for uncovering the potential functions of OsAK3 in rice growth and development
  • In addition, RNA-Seq data indicate that OsAK3 is involved in a variety of biological processes that regulate BR signaling and grain development in rice
  • An Adenylate Kinase OsAK3 Involves Brassinosteroid Signaling and Grain Length in Rice (Oryza sativa L.).
  • OsAK3 encodes an adenylate kinase, which regulates grain size by controlling cell expansion of rice spikelet glume
  • Among these mutants, mutant osak3 presents shorter grain length and dwarfing phenotype
  • Overexpression of OsAK3 resulted in longer grain length
  • Lamina inclination, coleoptile elongation and root inhibition experiments showed that the osak3 mutant was less sensitive to exogenous brassinolide (BL) treatment
• Connection
  • Adk-a~OsAK3, Adk-b~OsAK4, Molecular characterization of cDNA encoding for adenylate kinase of rice Oryza sativa L., Two types of genes (Adk-a, and Adk-b) encoding for adenylate kinase (AK, EC 2
  • Adk-a~OsAK3, Adk-b~OsAK4, Molecular characterization of cDNA encoding for adenylate kinase of rice Oryza sativa L., One of the genes, Adk-a, had 1154 bp sequences encoding 241 amino acid residues, while the other type, Adk-b, contained 1085 bp sequences encoding for 243 amino acid residues
  • Adk-a~OsAK3, Adk-b~OsAK4, Molecular characterization of cDNA encoding for adenylate kinase of rice Oryza sativa L., Homology between Adk-a and Adk-b was 73
  • Adk-a~OsAK3, GL3.1~qGL3-1~qGL3~OsPPKL1, An Adenylate Kinase OsAK3 Involves Brassinosteroid Signaling and Grain Length in Rice Oryza sativa L.., OsAK3 interacts with qGL3 in vivo and in vitro

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