OsFIGNL1

• Symbol: OsFIGNL1
• MSU: LOC_Os12g25720
• RAPdb: Os12g0443800

• The Rice AAA-ATPase OsFIGNL1 Is Essential for Male Meiosis., 2017, Front Plant Sci.
• FIGNL1 Inhibits Non-homologous Chromosome Association and Crossover Formation., 2022, Front Plant Sci.

Genbank accession number

• Here, we isolated and characterized the rice OsFIGNL1 (OsFidgetin-like 1) gene, encoding a conserved AAA-ATPase, and explored its function and importance in male meiosis and pollen formation
• The rice Osfignl1 mutant exhibited normal vegetative growth, but failed to produce seeds and displayed pollen abortion phenotype
• Phenotypic comparisons between the wild-type and Osfignl1 mutant demonstrated that OsFIGNL1 is required for anther development, and that the recessive mutation of this gene causes male sterility in rice
• Taken together, our results suggest that OsFIGNL1 plays an important role in regulation of male meiosis and anther development
• Complementation and CRISPR/Cas9 experiments demonstrated that wild-type OsFIGNL1 is responsible for the male sterility phenotype
• Male meiosis in the Osfignl1 mutant exhibited abnormal chromosome behavior, including chromosome bridges and multivalent chromosomes at diakinesis, lagging chromosomes, and chromosome fragments during meiosis

• DMC1A~DMC1~OsDMC1A, OsFIGNL1, The Rice AAA-ATPase OsFIGNL1 Is Essential for Male Meiosis., Yeast two-hybrid assays demonstrated OsFIGNL1 could interact with RAD51A1, RAD51A2, DMC1A, DMC1B, and these physical interactions were further confirmed by BiFC assay
• OsDMC1~DMC1B~OsDMC1B, OsFIGNL1, The Rice AAA-ATPase OsFIGNL1 Is Essential for Male Meiosis., Yeast two-hybrid assays demonstrated OsFIGNL1 could interact with RAD51A1, RAD51A2, DMC1A, DMC1B, and these physical interactions were further confirmed by BiFC assay