OsNDPK2,WSL12,YSS2

2015-01-20 | Categories genes  | Tags mitochondria  chloroplast  development  map-based cloning  salinity  tolerance  abiotic stress  ABA  stress  breeding  biotic stress  ABA  abscisic acid  stress tolerance  reactive oxygen species  chloroplast development  leaf  seedlings  growth  leaf development  seedling  photosynthesis  Chl biosynthesis  Kinase  plant growth 

• Information
  • Symbol: OsNDPK2,WSL12,YSS2
  • MSU: LOC_Os12g36194
  • RAPdb: Os12g0548300
• Publication
  • Physiological and Biochemical Characterization of Three Nucleoside Diphosphate Kinase Isozymes from Rice Oryza sativaL., 2014, Bioscience, Biotechnology and Biochemistry.
  • Enhanced expression of a gene encoding a nucleoside diphosphate kinase 1 OsNDPK1 in rice plants upon infection with bacterial pathogens, 2004, Mol Cells.
  • White stripe leaf 12 WSL12, encoding a nucleoside diphosphate kinase 2 OsNDPK2, regulates chloroplast development and abiotic stress response in rice Oryza sativa L.., 2016, Mol Breed.
  • A Young Seedling Stripe2 phenotype in rice is caused by mutation of a chloroplast-localized nucleoside diphosphate kinase 2 required for chloroplast biogenesis., 2017, Genet Mol Biol.
• Genbank accession number
  • AK070054
• Key message
  • Transient expression of green fluorescence protein-fused proteins in onion cells indicated that OsNDPK2 and OsNDPK3 are localized to plastid and mitochondria respectively, while OsNDPK1 is localized to the cytosol
  • Three different programs for predicting subcellular targeting indicated that OsNDPK1 and NP922751 were non-organellar, OsNDPK2 plastidic, and OsNDPK3 mitochondrial
  • The WSL12 protein localized to the chloroplast
  • These results indicate that the OsNDPK2 encoded by WSL12 plays an important role in chloroplast development and chlorophyll biosynthesis by regulating the expression levels of related genes
  • Map-based cloning revealed that the WSL12 locus encoded OsNDPK2, one of the three nucleoside diphosphate kinases (OsNDPKs)
  • The wsl12 mutant showed higher superoxide anion level and enhanced sensitivity to abscisic acid (ABA) and salinity
  • In addition, WSL12 also affects the response to abiotic stress, such as ABA and salinity in rice, and is beneficial to molecular breeding of stress tolerance
  • The transcription pattern of many genes involved in chlorophyll biosynthesis, ABA synthesis, light signaling pathway, reactive oxygen species-scavenging pathway and the other two OsNDPKs was altered in the wsl12 mutant
  • We isolated a rice mutant named yss2 (young seedling stripe2) with a striated seedling phenotype beginning from leaf 2 of delayed plant growth
  • Real-time RT-PCR analysis demonstrated that YSS2 transcripts accumulated highly in L4 sections at the early leaf development stage
  • Chlorotic yss2 seedlings have decreased pigment contents and impaired chloroplast development
  • Expression levels of genes associated with Chl biosynthesis and photosynthesis in yss2 were mostly decreased, but genes involved in chloroplast biogenesis were up-regulated compared to the wild type
  • Our overall data suggest that YSS2 has important roles in chloroplast biogenesis
  • The YSS2 allele encodes a NDPK2 (nucleoside diphosphate kinase 2) protein showing high similarity to other types of NDPKs
• Connection
  • OsNDPK1, OsNDPK2~WSL12~YSS2, Physiological and Biochemical Characterization of Three Nucleoside Diphosphate Kinase Isozymes from Rice Oryza sativaL., Transient expression of green fluorescence protein-fused proteins in onion cells indicated that OsNDPK2 and OsNDPK3 are localized to plastid and mitochondria respectively, while OsNDPK1 is localized to the cytosol
  • OsNDPK1, OsNDPK2~WSL12~YSS2, Physiological and Biochemical Characterization of Three Nucleoside Diphosphate Kinase Isozymes from Rice Oryza sativaL., Recombinant OsNDPK2 and OsNDPK3 showed lower optimum pH and higher stability under acidic pH than OsNDPK1
  • OsNDPK2~WSL12~YSS2, OsNDPK3, Physiological and Biochemical Characterization of Three Nucleoside Diphosphate Kinase Isozymes from Rice Oryza sativaL., Transient expression of green fluorescence protein-fused proteins in onion cells indicated that OsNDPK2 and OsNDPK3 are localized to plastid and mitochondria respectively, while OsNDPK1 is localized to the cytosol
  • OsNDPK2~WSL12~YSS2, OsNDPK3, Physiological and Biochemical Characterization of Three Nucleoside Diphosphate Kinase Isozymes from Rice Oryza sativaL., Recombinant OsNDPK2 and OsNDPK3 showed lower optimum pH and higher stability under acidic pH than OsNDPK1
  • OsNDPK2~WSL12~YSS2, OsNDPK3, Enhanced expression of a gene encoding a nucleoside diphosphate kinase 1 OsNDPK1 in rice plants upon infection with bacterial pathogens, Three other OsNDPK genes (NP922751, OsNDPK2 and OsNDPK3) found in databases were obtained by RTPCR
  • OsNDPK2~WSL12~YSS2, OsNDPK3, Enhanced expression of a gene encoding a nucleoside diphosphate kinase 1 OsNDPK1 in rice plants upon infection with bacterial pathogens, Three different programs for predicting subcellular targeting indicated that OsNDPK1 and NP922751 were non-organellar, OsNDPK2 plastidic, and OsNDPK3 mitochondrial
  • OsNDPK1, OsNDPK2~WSL12~YSS2, Enhanced expression of a gene encoding a nucleoside diphosphate kinase 1 OsNDPK1 in rice plants upon infection with bacterial pathogens, Three different programs for predicting subcellular targeting indicated that OsNDPK1 and NP922751 were non-organellar, OsNDPK2 plastidic, and OsNDPK3 mitochondrial

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