OsCD1,OsCSLD4,NRL1,ND1,sle1,DNL1

2015-01-20 | Categories genes  | Tags cell cycle  panicle  leaf  dwarf  cellulose  meristem  root  cell wall  vegetative  pollen  growth  culm  stem  stomata  height  architecture  cell division  anther  grain  salt  tolerance  ABA  salt tolerance  salt stress  stress  ABA  grain width  stress tolerance  stress response  ABA biosynthesis  osmotic stress  defense response  defense  plant growth  grain yield  biomass 

• Information
  • Symbol: OsCD1,OsCSLD4,NRL1,ND1,sle1,DNL1
  • MSU: LOC_Os12g36890
  • RAPdb: Os12g0555600
• Publication
  • Rice slender leaf 1 gene encodes cellulose synthase-like D4 and is specifically expressed in M-phase cells to regulate cell proliferation, 2013, J Exp Bot.
  • Rice cellulose synthase-like D4 is essential for normal cell-wall biosynthesis and plant growth, 2009, Plant J.
  • OsCD1 encodes a putative member of the cellulose synthase-like D sub-family and is essential for rice plant architecture and growth, 2011, Plant Biotechnol J.
  • Rice plants response to the disruption of OsCSLD4 gene, 2010, Plant Signal Behav.
  • Isolation and characterization of a rice mutant with narrow and rolled leaves, 2010, Planta.
  • Identification and characterization of NARROW AND ROLLED LEAF 1, a novel gene regulating leaf morphology and plant architecture in rice, 2010, Plant Mol Biol.
  • DNL1, encodes cellulose synthase-like D4, is a major QTL for plant height and leaf width in rice Oryza sativa L.., 2014, Biochem Biophys Res Commun.
  • Characterization of dwarf and narrow leaf dnl-4 mutant in rice, 2020, Plant Signal Behav.
  • Cadmium inhibits lateral root emergence in rice by disrupting OsPIN-mediated auxin distribution and the protective effect of OsHMA3, 2020, Plant Cell Physiol.
  • Cellulose Synthase-Like Protein OsCSLD4 Plays an Important Role in the Response of Rice to Salt Stress by Mediating ABA Biosynthesis to Regulate Osmotic Stress Tolerance , 2021, Plant Biotechnol J.
  • Variation of a major facilitator superfamily gene contributes to differential cadmium accumulation between rice subspecies., 2019, Nat Commun.
  • Rice cellulose synthase-like protein OsCSLD4 coordinates the trade-off between plant growth and defense., 2022, Front Plant Sci.
  • Cell Wall Matrix Polysaccharides Contribute to Salt-Alkali Tolerance in Rice., 2022, Int J Mol Sci.
• Genbank accession number
  • AK242601
• Key message
  • Double-target in situ hybridization and quantitative RT-PCR analyses revealed that SLE1 was expressed specifically during the M-phase of the cell cycle, and suggested that the cell-cycle regulation was altered in sle1 mutants
  • Analysis of OsCD1 promoter with GUS fusion expression shows that OsCD1 exhibits higher expression in young meristem tissues such as fresh roots, young panicle and stem apical meristem
  • To understand the molecular mechanism of leaf morphogenesis, we identified a rice mutant nrl1, which was characterized by a phenotype of narrow and rolled leaves
  • We isolated three allelic mutants of NARROW AND ROLLED LEAF 1 (nrl1) which showed phenotypes of reduced leaf width and semi-rolled leaves and different degrees of dwarfism
  • The NRL1 gene was mapped to the chromosome 12 and encodes the cellulose synthase-like protein D4 (OsCslD4)
  • We conclude that OsCslD4 encoded by NRL1 plays a critical role in leaf morphogenesis and vegetative development in rice
  • Cell wall composition analysis reveals that cellulose content and the level of xylose are significantly reduced in mature culm owing to loss of OsCD1 function
  • We report here an in-depth characterization of a narrow leaf and dwarf1 (nd1) rice mutant that shows significant reduction in plant growth due to retarded cell division
  • Map-based cloning revealed that SLE1 encodes the OsCSLD4 protein, which was identified previously from a narrow leaf and dwarf 1 mutant
  • Immunological studies and monosaccharide compositional and glycosyl linkage analyses explored several wall compositional effects caused by disruption of OsCSLD4, including alterations in the structure of arabinoxylan and the content of cellulose and homogalacturonan, which are distinct in the monocot grass species Oryza sativa (rice)
  • The nrl1 mutation was rescued by transformation with the wild-type cellulose synthase-like D4 gene
  • Accordingly, the cellulose synthase-like D4 gene was identified as the NRL1 gene
  • In addition to the size reduction of organs, sle1 mutants exhibited serious developmental defects in pollen formation, anther dehiscence, stomata formation, and cell arrangement in various tissues
  • OsCD1 encodes a putative member of the cellulose synthase-like D sub-family and is essential for rice plant architecture and growth
  • This study presents a detailed analysis of slender leaf 1 (sle1) mutants of rice that showed rolled and narrow leaf blades and a reduction in plant height
  • The narrow leaf blade of sle1 was caused by reduced cell proliferation beginning at the P3 primordial stage
  • Map-based cloning indicates that a member of the cellulose synthase-like D (CSLD) group is a candidate for OsCD1
  • Furthermore, sequence analysis indicates that OsCD1 contains the common D,D,D,QXXRW motif, which is a feature of the cellulose synthase-like super-family
  • OsCSLD4 is mainly expressed in tissues undergoing rapid growth
  • The inconsistent alterations in the two tissues and the observable structural defects in primary walls indicate that OsCSLD4 plays important roles in cell-wall formation and plant growth
  • NRL1 was transcribed in various tissues and was mainly expressed in panicles and internodes
  • Further study of OsCSLD4 is expected to yield new insight into the role of hemicelluloses in plant development
  • 08% phenotypic variation for plant height and leaf width, respectively, were located on the same interval of chromosome 12 flanking SSR markers RM519 and RM1103, and was named DNL1 (Dwarf and Narrowed Leaf 1)
  • The results of ORF analysis in target region and nucleotide sequence alignment indicated that DNL1 encodes cellulose synthase-like D4 protein, and a single nucleotide substitution (C2488T) in dnl1 result in decrease in plant height and leaf width
  • Intriguingly, the disruption of OsCSLD4 function decreased grain width and weight, while overexpression of OsCSLD4 increased grain width and weight
  • Cellulose Synthase-Like Protein OsCSLD4 Plays an Important Role in the Response of Rice to Salt Stress by Mediating ABA Biosynthesis to Regulate Osmotic Stress Tolerance
  • This study demonstrated that the OsCSLD4 function-disrupted mutant nd1 was sensitive to salt stress, but insensitive to ABA
  • Moreover, overexpression of OsCSLD4 can enhance rice ABA synthesis gene expression, increase ABA content, and improve rice salt tolerance, thus implying that OsCSLD4-regulated rice salt stress tolerance is mediated by ABA synthesis
  • The results from the transcriptome analysis showed that more osmotic stress responsive genes were impaired in nd1 than salt stress responsive genes, thus indicating that OsCSLD4 is involved in rice salt stress response through an ABA-induced osmotic response pathway
  • Natural variation in OsCd1 with a missense mutation Val449Asp is responsible for the divergence of rice grain Cd accumulation between indica and japonica
  • Rice cellulose synthase-like protein OsCSLD4 coordinates the trade-off between plant growth and defense.
  • Collectively, our data reveals the importance of OsCSLD4 in balancing the trade-off between rice growth and defense
  • Loss function of OsCSLD4 leads to a constitutive activation of defense response in rice
  • The OsCSLD4 function-disrupted mutant displayed reduced SSAS tolerance, biomass and grain yield, whereas the OsCSLD4 overexpression lines exhibited increased SSAS tolerance
  • Collectively, this study not only reveals the potential role of cell wall matrix polysaccharides in mediating SSAS tolerance, but also highlights applicable value of OsCSLD4 and the large-scale screening system in developing SSAS-tolerant rice
• Connection
  • OsCD1~OsCSLD4~NRL1~ND1~sle1~DNL1, RL9~SLL1~AH2~OsADD1, Isolation and characterization of a rice mutant with narrow and rolled leaves, NAL7 and SLL1 were found to be upregulated, whereas OsAGO7 were downregulated in the nrl1 mutant
  • OsAGO7~shl4~SHO2, OsCD1~OsCSLD4~NRL1~ND1~sle1~DNL1, Isolation and characterization of a rice mutant with narrow and rolled leaves, NAL7 and SLL1 were found to be upregulated, whereas OsAGO7 were downregulated in the nrl1 mutant
  • OsCD1~OsCSLD4~NRL1~ND1~sle1~DNL1, OsNRAMP1, Melatonin reduces cadmium accumulation via mediating the nitric oxide accumulation and increasing the cell wall fixation capacity of cadmium in rice., Furthermore, MT applied exogenously down-regulated the transcription level of Natural Resistance-Associated Macrophage Protein 1 (OsNRAMP1), OsNRAMP5, a major facilitator superfamily gene (OsCd1), and IRON-REGULATED TRANSPORTER 1 (OsIRT1), all of which were responsible for Cd intake, thus less Cd was entered into roots
  • OsCD1~OsCSLD4~NRL1~ND1~sle1~DNL1, OsNRAMP5, Melatonin reduces cadmium accumulation via mediating the nitric oxide accumulation and increasing the cell wall fixation capacity of cadmium in rice., Furthermore, MT applied exogenously down-regulated the transcription level of Natural Resistance-Associated Macrophage Protein 1 (OsNRAMP1), OsNRAMP5, a major facilitator superfamily gene (OsCd1), and IRON-REGULATED TRANSPORTER 1 (OsIRT1), all of which were responsible for Cd intake, thus less Cd was entered into roots

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