OsUEV1B

• Symbol: OsUEV1B
• MSU: LOC_Os12g41220
• RAPdb: Os12g0605400

• OsUEV1B, an Ubc enzyme variant protein, is required for phosphate homeostasis in rice., 2021, Plant J.

Genbank accession number

• Some Pi starvation inducible and phosphate transporter genes were upregulated in osuev1b mutant and OsUEV1B-RNAi plants in association with enhanced Pi acquisition, and representative Pi starvation responses, including stimulation of acid phosphatase activity and root hair growth, were also activated in the presence of sufficient Pi
• In the present study, we found OsUEV1B was specifically inhibited by Pi deficiency, and was localized in the nucleus and cytoplasm
• Our results indicate that OsUEV1B is required for rice phosphate homeostasis
• Both osuev1b mutant and OsUEV1B-RNA interference (RNAi) lines displayed serious symptoms of toxicity due to Pi overaccumulation
• Moreover, overexpression of OsVDAC1 in osuev1b partially restored its high Pi concentration to a level between those of osuev1b and DongJin

• ICS1~OsVDAC1~OsICS1, OsUEV1B, OsUEV1B, an Ubc enzyme variant protein, is required for phosphate homeostasis in rice., A yeast two-hybrid screen revealed an interaction between OsUEV1B and OsVDAC1, which was confirmed by bimolecular fluorescence complementation and firefly split-luciferase complementation assays
• ICS1~OsVDAC1~OsICS1, OsUEV1B, OsUEV1B, an Ubc enzyme variant protein, is required for phosphate homeostasis in rice., Under sufficient Pi conditions, similar to osuev1b, a mutation in OsVDAC1 resulted in significantly greater Pi concentrations in the roots and second leaves, improved acid phosphatase activity, and enhanced expression of the Pi starvation inducible and phosphate transporter genes compared with wild-type DongJin, whereas overexpression of OsVDAC1 had the opposite effects
• ICS1~OsVDAC1~OsICS1, OsUEV1B, OsUEV1B, an Ubc enzyme variant protein, is required for phosphate homeostasis in rice., OsUEV1B or OsVDAC1 knockout reduced the mitochondrial membrane potential and adenosine triphosphate levels
• ICS1~OsVDAC1~OsICS1, OsUEV1B, OsUEV1B, an Ubc enzyme variant protein, is required for phosphate homeostasis in rice., Moreover, overexpression of OsVDAC1 in osuev1b partially restored its high Pi concentration to a level between those of osuev1b and DongJin
• OsUBC13, OsUEV1B, OsUEV1B, an Ubc enzyme variant protein, is required for phosphate homeostasis in rice., The ubiquitin-conjugating enzyme variant protein OsUEV1B was previously found to interact with OsUbc13 and mediate lysine63-linked polyubiquitination