- Information
- Symbol: OsSpo11-4,OsSPO11D
- MSU: LOC_Os12g42760
- RAPdb: Os12g0622500
- Publication
- The double-stranded break-forming activity of plant SPO11s and a novel rice SPO11 revealed by a Drosophila bioassay, 2012, BMC Mol Biol.
- OsSpo11-4, a rice homologue of the archaeal TopVIA protein, mediates double-strand DNA cleavage and interacts with OsTopVIB, 2011, PLoS One.
- Gene Expression Dynamics in Rice Peduncles at the Heading Stage, 2020, Front Genet.
- Genbank accession number
- Key message
- Quantitative analyses of the rice SPO11 transcripts revealed the specific increase in OsSPO11D mRNA in the anthers containing meiotic pollen mother cells
- Furthermore, we identified a novel SPO11 homologue, OsSPO11D, with robust DSB activity and a possible meiotic function
- We further analyzed the biological function of OsSpo11-4 by RNA interference and found that down-regulated expression of OsSpo11-4 led to defects in male meiosis, indicating OsSpo11-4 is required for meiosis
- Connection
- OsSpo11-4~OsSPO11D, OsSpo11-5, OsSpo11-4, a rice homologue of the archaeal TopVIA protein, mediates double-strand DNA cleavage and interacts with OsTopVIB, Here, we further identified two novel Spo11/TopVIA homologues (named OsSpo11-4 and OsSpo11-5, respectively) that exist just in the monocot model plant Oryza sativa, indicating that at least five Spo11/TopVIA homologues are present in the rice genome
- OsSpo11-4~OsSPO11D, OsSpo11-5, OsSpo11-4, a rice homologue of the archaeal TopVIA protein, mediates double-strand DNA cleavage and interacts with OsTopVIB, To reveal the biochemical function of the two novel Spo11/TopVIA homologues, we first examined the interactions among OsSpo11-1, OsSpo11-4, OsSpo11-5, and OsTopVIB by yeast two-hybrid assay
- OsSpo11-4~OsSPO11D, TOP6A2~OsTOP6A2~OsSPO11-2, Assessment of the roles of OsSPO11-2 and OsSPO11-4 in rice meiosis using CRISPR/Cas9 mutagenesis, Assessment of the roles of OsSPO11-2 and OsSPO11-4 in rice meiosis using CRISPR/Cas9 mutagenesis
- OsSpo11-4~OsSPO11D, TOP6A2~OsTOP6A2~OsSPO11-2, Assessment of the roles of OsSPO11-2 and OsSPO11-4 in rice meiosis using CRISPR/Cas9 mutagenesis, To clarify the roles of these SPO11 paralogs, we used CRISPR/Cas9 mutagenesis to induce null mutants in OsSPO11-1, OsSPO11-2 and OsSPO11-4
- OsSpo11-4~OsSPO11D, TOP6A2~OsTOP6A2~OsSPO11-2, Assessment of the roles of OsSPO11-2 and OsSPO11-4 in rice meiosis using CRISPR/Cas9 mutagenesis, Altogether, this study demonstrates that whereas OsSPO11-1 and OsSPO11-2 are the likely orthologs of Arabidopsis AtSPO11-1 and AtSPO11-2, the additional OsSPO11-4 gene has no major role in wild-type rice meiosis
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