OsSUT2,OsSUT2M

2015-01-20 | Categories genes  | Tags leaf  grain  panicle  spikelet  root  growth  transporter  tiller  seed  vegetative  flower  tiller number  lateral root  grain weight  seed development  height  pollen  grain size  grain number  sugar  plasma membrane  grain-filling  sugar transport 

• Information
  • Symbol: OsSUT2,OsSUT2M
  • MSU: LOC_Os12g44380
  • RAPdb: Os12g0641400
• Publication
  • Sugar transporters involved in flowering and grain development of rice, 2001, Journal of Plant Physiology.
  • Impaired function of the tonoplast-localized sucrose transporter in rice, OsSUT2, limits the transport of vacuolar reserve sucrose and affects plant growth, 2011, Plant Physiol.
  • The rice pentatricopeptide repeat protein RF5 restores fertility in Hong-Lian cytoplasmic male-sterile lines via a complex with the glycine-rich protein GRP162, 2012, Plant Cell.
  • Cloning and expression analysis of rice sucrose transporter genes OsSUT2M and OsSUT5Z, 2008, J Integr Plant Biol.
  • The sucrose transporter gene family in rice, 2003, Plant Cell Physiol.
  • OsRRM, an RNA-Binding Protein, Modulates Sugar Transport in Rice Oryza sativa L., 2020, Front Plant Sci.
  • MATE transporter GFD1 cooperates with sugar transporters, mediates carbohydrate partitioning and controls grain-filling duration, grain size and number in rice., 2022, Plant Biotechnol J.
• Genbank accession number
  • DQ072592
• Key message
  • Analysis of the OsSUT2 promoter::beta-glucuronidase transgenic rice indicated that this gene is highly expressed in leaf mesophyll cells, emerging lateral roots, pedicels of fertilized spikelets, and cross cell layers of seed coats
  • In vegetative organs, transcripts of OsSUT2M were higher in source leaf blades than in other organs at the same development stage, whereas transcripts of OsSUT5Z were less traceable in all organs investigated
  • To investigate sugar transport processes during flowering and in developing grains of rice, we newly isolated two genomic clonesOsSUT2(Oryzasativasucrosetransporter 2) andOsMST5(Oryzasativamonosaccharidetransporter 5) and their corresponding cDNAs
  • The ossut2 mutant exhibited a growth retardation phenotype with a significant reduction in tiller number, plant height, 1,000-grain weight, and root dry weight compared with the controls, the wild type, and complemented transgenic lines
  • Northern blot analysis revealed thatOsSUT2andOsMST5mRNA accumulates in panicles before pollination
  • Impaired function of the tonoplast-localized sucrose transporter in rice, OsSUT2, limits the transport of vacuolar reserve sucrose and affects plant growth
  • Two sucrose transporter (SUT) cDNAs, OsSUT2M and OsSUT5Z, were isolated from rice (Oryza sativa L
  • The TopPred program suggested that both sucrose transporter proteins, OsSUT2M and OsSUT5Z, consist of potentially 12 transmembrane domains
  • Cloning and expression analysis of rice sucrose transporter genes OsSUT2M and OsSUT5Z
  • These data presented suggest that bothOsSUT2andOsMST5 play a role during the development at the early stage of the seed development
  • In this paper we report the identification, cloning and expression analysis of four putative sucrose transporter (SUT) genes from rice, designated OsSUT2, 3, 4 and 5
  • In situ hybridization analysis showed thatOsSUT2transcript is specific to the developing pollen
  • Genetic analyses showed that GFD1 might control grain-filling duration through OsSWEET4, adjust grain size with OsSUT2 and synergistically modulate grain number per panicle with both OsSUT2 and OsSWEET4
  • GFD1 protein was partially localized on the plasma membrane and in the Golgi apparatus, and was finally verified to interact with two sugar transporters, OsSWEET4 and OsSUT2
• Connection
  • OsMST5, OsSUT2~OsSUT2M, Sugar transporters involved in flowering and grain development of rice, To investigate sugar transport processes during flowering and in developing grains of rice, we newly isolated two genomic clonesOsSUT2(Oryzasativasucrosetransporter 2) andOsMST5(Oryzasativamonosaccharidetransporter 5) and their corresponding cDNAs
  • OsMST5, OsSUT2~OsSUT2M, Sugar transporters involved in flowering and grain development of rice, OsSUT2 andOsMST5are encoded by open reading frames of 1485 and 1557 bp encoding 495 and 519amino acids, respectively
  • OsMST5, OsSUT2~OsSUT2M, Sugar transporters involved in flowering and grain development of rice, Northern blot analysis revealed thatOsSUT2andOsMST5mRNA accumulates in panicles before pollination
  • OsMST5, OsSUT2~OsSUT2M, Sugar transporters involved in flowering and grain development of rice, These data presented suggest that bothOsSUT2andOsMST5 play a role during the development at the early stage of the seed development
  • OsSUT1, OsSUT2~OsSUT2M, Sugar transporters involved in flowering and grain development of rice, On the other handOsSUT1 was expressed at the early stage of the grain development, suggesting a different physiological role compared to Os SUT2
  • OsSUT2~OsSUT2M, OsSUT5~OsSUT5Z, Cloning and expression analysis of rice sucrose transporter genes OsSUT2M and OsSUT5Z, Two sucrose transporter (SUT) cDNAs, OsSUT2M and OsSUT5Z, were isolated from rice (Oryza sativa L
  • OsSUT2~OsSUT2M, OsSUT5~OsSUT5Z, Cloning and expression analysis of rice sucrose transporter genes OsSUT2M and OsSUT5Z, The TopPred program suggested that both sucrose transporter proteins, OsSUT2M and OsSUT5Z, consist of potentially 12 transmembrane domains
  • OsSUT2~OsSUT2M, OsSUT5~OsSUT5Z, Cloning and expression analysis of rice sucrose transporter genes OsSUT2M and OsSUT5Z, Semi-quantitative RT-PCR was carried out to investigate the gene expression patterns of OsSUT2M and OsSUT5Z
  • OsSUT2~OsSUT2M, OsSUT5~OsSUT5Z, Cloning and expression analysis of rice sucrose transporter genes OsSUT2M and OsSUT5Z, In vegetative organs, transcripts of OsSUT2M were higher in source leaf blades than in other organs at the same development stage, whereas transcripts of OsSUT5Z were less traceable in all organs investigated
  • OsSUT2~OsSUT2M, OsSUT5~OsSUT5Z, Cloning and expression analysis of rice sucrose transporter genes OsSUT2M and OsSUT5Z, Cloning and expression analysis of rice sucrose transporter genes OsSUT2M and OsSUT5Z
  • GS3.1~DG1~GFD1, OsSUT2~OsSUT2M, MATE transporter GFD1 cooperates with sugar transporters, mediates carbohydrate partitioning and controls grain-filling duration, grain size and number in rice., GFD1 protein was partially localized on the plasma membrane and in the Golgi apparatus, and was finally verified to interact with two sugar transporters, OsSWEET4 and OsSUT2
  • GS3.1~DG1~GFD1, OsSUT2~OsSUT2M, MATE transporter GFD1 cooperates with sugar transporters, mediates carbohydrate partitioning and controls grain-filling duration, grain size and number in rice., Genetic analyses showed that GFD1 might control grain-filling duration through OsSWEET4, adjust grain size with OsSUT2 and synergistically modulate grain number per panicle with both OsSUT2 and OsSWEET4
  • OsSUT2~OsSUT2M, OsSWEET4, MATE transporter GFD1 cooperates with sugar transporters, mediates carbohydrate partitioning and controls grain-filling duration, grain size and number in rice., GFD1 protein was partially localized on the plasma membrane and in the Golgi apparatus, and was finally verified to interact with two sugar transporters, OsSWEET4 and OsSUT2
  • OsSUT2~OsSUT2M, OsSWEET4, MATE transporter GFD1 cooperates with sugar transporters, mediates carbohydrate partitioning and controls grain-filling duration, grain size and number in rice., Genetic analyses showed that GFD1 might control grain-filling duration through OsSWEET4, adjust grain size with OsSUT2 and synergistically modulate grain number per panicle with both OsSUT2 and OsSWEET4

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