OsPUL

2015-01-20 | Categories genes  | Tags starch  grain filling  seed development  branching  endosperm  seed germination  seed  grain 

• Information
  • Symbol: OsPUL
  • MSU: None
  • RAPdb: None
• Publication
  • Characterization of expression of the OsPUL gene encoding a pullulanase-type debranching enzyme during seed development and germination in rice, 2009, Plant Physiol Biochem.
  • Structural and enzymatic characterization of the isoamylase1 homo-oligomer and the isoamylase1-isoamylase2 hetero-oligomer from rice endosperm, 2006, Planta.
  • Rice debranching enzyme isoamylase3 facilitates starch metabolism and affects plastid morphogenesis, 2011, Plant Cell Physiol.
  • Sequence variations in OsAGPase significantly associated with amylose content and viscosity properties in rice Oryza sativa L.., 2012, Genet Res (Camb).
• Genbank accession number
  • AB012915
• Key message
  • The expression of the OsPUL gene was maintained at a significant level during the entire grain filling period and in the late stage of seed germination, which coincided with its involvement in starch anabolism and catabolism
  • The OsPUL gene maintained a relatively high expression level during the entire period of seed development, and peaked in the middle and late stages
  • Characterization of expression of the OsPUL gene encoding a pullulanase-type debranching enzyme during seed development and germination in rice
  • In addition, the 1177-bp OsPUL promoter sequence was cloned, and some endosperm-specific motifs such as the GCN4 and AACA motifs were observed to exist in this region
  • The data from both histochemical and fluorometric analyses showed that the OsPUL promoter was capable of driving the target gene to have a high level of endosperm-specific expression
  • Furthermore, the seed germination experiment showed that the OsPUL promoter actively functions in the late stage of seed germination
  • In the present study, we performed a quantitative real-time PCR (Q-PCR) analysis of the expression pattern of the OsPUL gene encoding a pullulanase-type DBE in different tissues as well as in seeds at different developmental stages
• Connection
  • FLO2, OsPUL, Three novel alleles of FLOURY ENDOSPERM2 FLO2 confer dull grains with low amylose content in rice., The allelic diversity of flo2 perturbed the expression of starch synthesis-related genes including OsAGPL2, OsAGPS2b, OsGBSSI, OsBEI, OsBEIIb, OsISA1, and OsPUL

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