OsmiR535

| Categories genes  | Tags panicle  grain  grain length  architecture  branching  height  plant height  internode length  panicle architecture  grain width  seedlings  plant development  drought  salinity  tolerance  ethylene  stress  breeding  abscisic acid  stress response  ER stress 
  • Information
    • Symbol: OsmiR535
    • MSU: None
    • RAPdb: None
  • Publication

  • Genbank accession number

  • Key message
    • The multiple roles of OsmiR535 in modulating plant height, panicle branching and grain shape.
    • Furthermore, OsmiR535 overexpression imposed great influence in panicle architecture, such as more but shorter panicles, and fewer primary/secondary panicle branches
    • Through quantitative real-time PCR analyses, we further revealed that OsmiR535 overexpression repressed the expression of OsSPL7/12/16, as well as the OsSPLs downstream panicle related genes, including OsPIN1B, OsDEP1, OsLOG and OsSLR1
    • Taken together, our findings suggest that OsmiR535 multiply modulates plant height, panicle architecture and grain shape possibly by regulating OsSPLs genes in rice
    • Moreover, OsmiR535 overexpression increased the grain length, but did not affect grain width
    • Expectedly, OsmiR535 overexpression in rice reduced plant height by decreasing the 1st and 2nd internode length
    • In addition, the overexpression of OsmiR535 significantly reduced the survival rate of rice seedlings during PEG and dehydration post-stress recovery
    • OsmiR535 is involved in regulating the cold-stress response, modulating plant development, and determining panicle architecture and grain length
    • However, the role that OsmiR535 plays in plant responses to drought and salinity are elusive
    • Moreover, our practical application of CRISPR/Cas9 mediated genome editing created a homozygous 5 bp deletion in the coding sequence of OsmiR535, demonstrating that OsmiR535 could be a useful genetic editing target for drought and salinity tolerance and a new marker for molecular breeding of Oryza sativa
    • With transgenic and CRISPR/Cas9 knockout system techniques, our results verified that either inhibition or knockout of OsmiR535 in rice could enhance the tolerance of plants to NaCl, ABA, dehydration and PEG stresses
    • In the current study, molecular and genetic engineering techniques were used to elucidate the possible role of OsmiR535 in response to NaCl, PEG(Poly ethylene glycol), ABA(Abscisic acid), and dehydration stresses
    • Our results demonstrated that OsmiR535 negatively regulates the stress response in rice
    • Our results showed that OsmiR535 is induced under stressed conditions as compared to control
  • Connection

Prev     Next