- Information
- Symbol: OsCKX4
- MSU: LOC_Os01g71310
- RAPdb: Os01g0940000
- Publication
- CYTOKININ OXIDASE/DEHYDROGENASE4 Integrates Cytokinin and Auxin Signaling to Control Rice Crown Root Formation., 2014, Plant Physiol.
- Phenotypic, Transcriptomic, and Metabolomic Signatures of Root-Specifically Overexpressed OsCKX2 in Rice, 2020, Front Plant Sci.
- Endoplasmic Reticulum-Localized PURINE PERMEASE1 Regulates Plant Height and Grain Weight by Modulating Cytokinin Distribution in Rice, 2020, Front Plant Sci.
- The Amino Acid Transporter OsAAP4 Contributes to Rice Tillering and Grain Yield by Regulating Neutral Amino Acid Allocation through Two Splicing Variants, 2021, Rice (N Y).
- OsDOF11 Promotes Crown Root Formation via Cytokinin in Oryza Sativa., 2022, Front Biosci (Landmark Ed).
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Genbank accession number
- Key message
- OsCKX4 is predominantly expressed in leaf blades and roots
- Overexpression and RNA interference of OsCKX4 confirmed that OsCKX4 plays a positive role in crown root formation
- Moreover, expression analysis revealed a significant alteration in the expression of auxin-related genes in the ren1-D mutants, indicating that the OsCKX4 mediates crown root development by integrating the interaction between cytokinin and auxin
- Transgenic plants harboring OsCKX4 under the control of the root-specific promoter RCc3 displayed enhanced root development without affecting their shoot parts, suggesting that this strategy could be a powerful tool in rice root engineering
- OsCKX4 is induced by exogenous auxin and cytokinin in the roots
- Furthermore, one-hybrid assays revealed that OsCKX4 is a direct binding target of both the auxin response factor OsARF25 and the cytokinin response regulators OsRR2 and OsRR3
- Molecular and genetic analyses revealed that these phenotypes are caused by the activation of a cytokinin oxidase/dehydrogenase (CKX) family gene, OsCKX4
- Connection
- OsARF25, OsCKX4, CYTOKININ OXIDASE/DEHYDROGENASE4 Integrates Cytokinin and Auxin Signaling to Control Rice Crown Root Formation., Furthermore, one-hybrid assays revealed that OsCKX4 is a direct binding target of both the auxin response factor OsARF25 and the cytokinin response regulators OsRR2 and OsRR3
- OsCKX4, OsRR2, CYTOKININ OXIDASE/DEHYDROGENASE4 Integrates Cytokinin and Auxin Signaling to Control Rice Crown Root Formation., Furthermore, one-hybrid assays revealed that OsCKX4 is a direct binding target of both the auxin response factor OsARF25 and the cytokinin response regulators OsRR2 and OsRR3
- OsCKX4, OsRR3, CYTOKININ OXIDASE/DEHYDROGENASE4 Integrates Cytokinin and Auxin Signaling to Control Rice Crown Root Formation., Furthermore, one-hybrid assays revealed that OsCKX4 is a direct binding target of both the auxin response factor OsARF25 and the cytokinin response regulators OsRR2 and OsRR3
- D6~OSH15~Oskn3~RLB, OsCKX4, RLB RICE LATERAL BRANCH recruits PRC2-mediated H3K27 tri-methylation on OsCKX4 to regulate lateral branching., RLB (RICE LATERAL BRANCH) recruits PRC2-mediated H3K27 tri-methylation on OsCKX4 to regulate lateral branching.
- D6~OSH15~Oskn3~RLB, OsCKX4, RLB RICE LATERAL BRANCH recruits PRC2-mediated H3K27 tri-methylation on OsCKX4 to regulate lateral branching., RLB directly binds to the promoter and represses the transcription of OsCKX4, a cytokinin oxidase gene with high abundance in panicle branch meristem
- D6~OSH15~Oskn3~RLB, OsCKX4, RLB RICE LATERAL BRANCH recruits PRC2-mediated H3K27 tri-methylation on OsCKX4 to regulate lateral branching., OsCKX4 over-expression lines phenocopied rlb which showed up-regulated OsCKX4 levels
- D6~OSH15~Oskn3~RLB, OsCKX4, RLB RICE LATERAL BRANCH recruits PRC2-mediated H3K27 tri-methylation on OsCKX4 to regulate lateral branching., Meanwhile, RLB physically binds to Polycomb repressive complex 2 (PRC2) component OsEMF2b and co-localized with H3K27me3, a suppressing histone modification mediated by PRC2, in the OsCKX4 promoter
- D6~OSH15~Oskn3~RLB, OsCKX4, RLB RICE LATERAL BRANCH recruits PRC2-mediated H3K27 tri-methylation on OsCKX4 to regulate lateral branching., We proposed that RLB recruits PRC2 to the OsCKX4 promoter to epigenetically repress its transcription, which suppresses the catabolism of cytokinin, thereby promoting rice lateral branching
- OsCKX4, OsDOF11, The sucrose transport regulator OsDOF11 mediates cytokinin degradation during rice development., Moreover, mutation of osckx4 in the osdof11 osckx4 double mutant rescued the semi-dwarf phenotype of the osdof11 mutant
- OsCKX4, OsDOF11, The sucrose transport regulator OsDOF11 mediates cytokinin degradation during rice development., Interestingly, exogenous application of kinetin promoted OsDOF11 expression earlier than OsCKX4, and overexpression of O
- OsCKX4, OsDOF11, The sucrose transport regulator OsDOF11 mediates cytokinin degradation during rice development., Taken together, our results suggest a model in which both a sucrose transport regulator (OsDOF11) and cytokinin via OsCKX4 establish a feedback loop to maintain dynamic tissue homeostasis
- CRL1~ARL1, OsCKX4, WOX11 and CRL1 act synergistically to promote crown root development by maintaining cytokinin homeostasis in rice., Eletrophoretic mobility shift assays (EMSAs), transient expression and chromatin immunoprecipitation qPCR (ChIP-qPCR) suggested that WOX11 and CRL1 directly bind to OsCKX4 to regulate its expression during crown root development
- CRL1~ARL1, OsCKX4, WOX11 and CRL1 act synergistically to promote crown root development by maintaining cytokinin homeostasis in rice., CRL1 enhances OsCKX4 activation through direct interaction with WOX11 at root emergence and elongation stages
- CRL1~ARL1, OsCKX4, WOX11 and CRL1 act synergistically to promote crown root development by maintaining cytokinin homeostasis in rice., Increased expression of OsCKX4 could partially complement the crown root phenotypes of both crl1 and wox11 mutants
- OsCKX4, WOX11, WOX11 and CRL1 act synergistically to promote crown root development by maintaining cytokinin homeostasis in rice., Eletrophoretic mobility shift assays (EMSAs), transient expression and chromatin immunoprecipitation qPCR (ChIP-qPCR) suggested that WOX11 and CRL1 directly bind to OsCKX4 to regulate its expression during crown root development
- OsCKX4, WOX11, WOX11 and CRL1 act synergistically to promote crown root development by maintaining cytokinin homeostasis in rice., CRL1 enhances OsCKX4 activation through direct interaction with WOX11 at root emergence and elongation stages
- OsCKX4, WOX11, WOX11 and CRL1 act synergistically to promote crown root development by maintaining cytokinin homeostasis in rice., Increased expression of OsCKX4 could partially complement the crown root phenotypes of both crl1 and wox11 mutants
- OsCKX4, OsDOF11, OsDOF11 Promotes Crown Root Formation via Cytokinin in Oryza Sativa., Previously, we reported negative feedback regulation loop between Oryza sativa DNA BINDING WITH ONE FINGER 11 (OsDOF11) and cytokinin by Oryza sativa CYTOKININ OXIDASE/DEHYDROGENASE 4 (OsCKX4) in rice development
- OsCKX4, OsDOF11, OsDOF11 Promotes Crown Root Formation via Cytokinin in Oryza Sativa., The expression level of WUSCHEL-related homeobox (OsWOX11), A-type response regulator 2 (OsRR2), OsRR3, and OsCKX4 were decreased in osdof11-1, as well as in OsDOF11 RNA interference 9 mutants (RNAi-9 lines)
- OsCKX4, OsRR2, OsDOF11 Promotes Crown Root Formation via Cytokinin in Oryza Sativa., The expression level of WUSCHEL-related homeobox (OsWOX11), A-type response regulator 2 (OsRR2), OsRR3, and OsCKX4 were decreased in osdof11-1, as well as in OsDOF11 RNA interference 9 mutants (RNAi-9 lines)
- OsCKX4, OsRR3, OsDOF11 Promotes Crown Root Formation via Cytokinin in Oryza Sativa., The expression level of WUSCHEL-related homeobox (OsWOX11), A-type response regulator 2 (OsRR2), OsRR3, and OsCKX4 were decreased in osdof11-1, as well as in OsDOF11 RNA interference 9 mutants (RNAi-9 lines)
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