- Information
- Symbol: OsDEF7,CAL1,OsAFP1
- MSU: LOC_Os02g41904
- RAPdb: Os02g0629800
- Publication
- Gene expression analysis, subcellular localization, and in planta antimicrobial activity of rice Oryza sativa L. defensin 7 and 8., 2018, Plant Physiol Biochem.
- A defensin-like protein drives cadmium efflux and allocation in rice., 2018, Nat Commun.
- Rice Defensin OsAFP1 is a New Drug Candidate against Human Pathogenic Fungi., 2018, Sci Rep.
- Partial peptides from rice defensin OsAFP1 exhibited antifungal activity against the rice blast pathogen Pyricularia oryzae., 2017, J Pestic Sci.
- Crystal structure of rice defensin OsAFP1 and molecular insight into lipid-binding., 2020, J Biosci Bioeng.
- Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, 2020, PLoS One.
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Genbank accession number
- Key message
- CAL1 is expressed preferentially in root exodermis and xylem parenchyma cells
- We provide evidence that CAL1 acts by chelating Cd in the cytosol and facilitating Cd secretion to extracellular spaces, hence lowering cytosolic Cd concentration while driving long-distance Cd transport via xylem vessels
- OsAFP1 exerted fungicidal activity against Candida albicans, the most common pathogenic fungus in humans, at 4<U+2009>
M concentration, but it did not inhibit the growth of human pathogenic bacteria - Immunohistochemistry showed that the OsAFP1 target molecule was located in the cell wall
- Further, PI uptake and apoptosis assays suggested that OsAFP1 exerts its antifungal activity by inducing apoptosis of target cells
- Partial peptides from rice defensin OsAFP1 exhibited antifungal activity against the rice blast pathogen Pyricularia oryzae.
- In lipid-binding analyses performed using nitrocellulose membranes immobilized with various membrane lipid components, OsAFP1 was found to bind to phosphatidylinositols (PIPs) harboring phosphate groups, particularly PI(3)P
- Connection
- CAL2, OsDEF7~CAL1~OsAFP1, Overexpression of a Defensin-Like Gene CAL2 Enhances Cadmium Accumulation in Plants., CAL2 shared 66% similarity with CAL1, and its mRNA accumulation is mainly observed in roots and is unaffected by Cd stress, but its transcription level was lower than that of CAL1 based on the relative expression of CAL2/Actin1 observed in this study and that reported previously
- OsDEF7~CAL1~OsAFP1, OsNRAMP5, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Fe, Mn, and Cd transporters such as CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, OsHMA3, and OsNAAT1 regulate uptake of Cd in rice
- OsDEF7~CAL1~OsAFP1, OsNRAMP5, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Meanwhile, application of sufficient doses of Fe and Cd in solution culture increased RE of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 in roots, whereas expression level of OsHMA3 was decreased
- OsDEF7~CAL1~OsAFP1, OsNRAMP5, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Similarly, expression level of CAL1, OsNRAMP5, and OsNRAMP1 significantly increased in roots in high Cd and Mn deficient treatments
- OsDEF7~CAL1~OsAFP1, OsNRAMP5, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, This may be concluded that the Cd increases expression of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 but decreases OsHMA3 expression in rice roots, which resulted in increased Cd uptake in hydroponically grown rice
- OsDEF7~CAL1~OsAFP1, OsNRAMP1, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Fe, Mn, and Cd transporters such as CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, OsHMA3, and OsNAAT1 regulate uptake of Cd in rice
- OsDEF7~CAL1~OsAFP1, OsNRAMP1, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Meanwhile, application of sufficient doses of Fe and Cd in solution culture increased RE of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 in roots, whereas expression level of OsHMA3 was decreased
- OsDEF7~CAL1~OsAFP1, OsNRAMP1, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Similarly, expression level of CAL1, OsNRAMP5, and OsNRAMP1 significantly increased in roots in high Cd and Mn deficient treatments
- OsDEF7~CAL1~OsAFP1, OsNRAMP1, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, This may be concluded that the Cd increases expression of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 but decreases OsHMA3 expression in rice roots, which resulted in increased Cd uptake in hydroponically grown rice
- OsDEF7~CAL1~OsAFP1, OsIRT1, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Fe, Mn, and Cd transporters such as CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, OsHMA3, and OsNAAT1 regulate uptake of Cd in rice
- OsDEF7~CAL1~OsAFP1, OsIRT1, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Meanwhile, application of sufficient doses of Fe and Cd in solution culture increased RE of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 in roots, whereas expression level of OsHMA3 was decreased
- OsDEF7~CAL1~OsAFP1, OsIRT1, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, This may be concluded that the Cd increases expression of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 but decreases OsHMA3 expression in rice roots, which resulted in increased Cd uptake in hydroponically grown rice
- OsDEF7~CAL1~OsAFP1, OsNAAT1, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Fe, Mn, and Cd transporters such as CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, OsHMA3, and OsNAAT1 regulate uptake of Cd in rice
- OsDEF7~CAL1~OsAFP1, OsNAAT1, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Meanwhile, application of sufficient doses of Fe and Cd in solution culture increased RE of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 in roots, whereas expression level of OsHMA3 was decreased
- OsDEF7~CAL1~OsAFP1, OsNAAT1, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, This may be concluded that the Cd increases expression of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 but decreases OsHMA3 expression in rice roots, which resulted in increased Cd uptake in hydroponically grown rice
- OsDEF7~CAL1~OsAFP1, qCdT7~OsHMA3, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Fe, Mn, and Cd transporters such as CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, OsHMA3, and OsNAAT1 regulate uptake of Cd in rice
- OsDEF7~CAL1~OsAFP1, qCdT7~OsHMA3, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Meanwhile, application of sufficient doses of Fe and Cd in solution culture increased RE of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 in roots, whereas expression level of OsHMA3 was decreased
- OsDEF7~CAL1~OsAFP1, qCdT7~OsHMA3, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, This may be concluded that the Cd increases expression of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 but decreases OsHMA3 expression in rice roots, which resulted in increased Cd uptake in hydroponically grown rice
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