PGL2,OsBUL1,OsbHLH173

2015-01-20 | Categories genes | Tags grain length palea grain size brassinosteroid grain lemma leaf cell elongation height plant height transcriptional activator architecture iaa BR IAA plant architecture leaf angle

Information
- Symbol: PGL2,OsBUL1,OsbHLH173
- MSU: LOC_Os02g51320
- RAPdb: Os02g0747900
Publication
Genbank accession number
- FP100305
Key message
- Here, we report the involvement of another atypical bHLH protein gene, POSITIVE REGULATOR OF GRAIN LENGTH 2 (PGL2), in the regulation of rice grain length
- Over-expression of PGL2 in the lemma/palea increased grain length and weight in correlation with the level of transgene expression
- PGL2 interacts with a typical bHLH protein APG, a negative regulator of rice grain length and weight, in vitro and in vivo
- It was reported that overexpression of BU1 (BRASSINOSTEROID UPREGULATED 1), the closest homolog of PGL2, caused an increase in grain length
- These findings suggest that PGL2 and PGL1 redundantly suppress the function of APG by forming heterodimers to positively regulate the rice grain length, while the pathway through which BU1, the closest homolog of PGL2, controls grain length is independent of APG
- Observation of the inner epidermal cells of lemma of PGL2-overexpressing lines revealed that the long grain size is caused by an increase in cell length
- Rice leaf angle and grain size are affected by the OsBUL1 transcriptional activator complex
- Transgenic rice plants expressing OsBC1 under the control of OsBUL1 promoter showed increased grain size as well as leaf bending while transgenic lines for dsRNAi and/or expressing a dominant repressor form of OsBC1 displayed reduced plant height and grain size
- OsBUL1 knockout mutant (osbul1) and transgenic rice for double stranded RNA interference (dsRNAi) of OsBUL1 produced erected leaves with smaller grains whereas OsBUL1 overexpressors and an activation tagging line of OsBUL1 exhibited increased laminar inclination and grain size
- To understand the molecular network of OsBUL1 function in rice, we isolated a novel OsBUL1-interacting protein, LO9-177, an uncharacterized protein containing a KxDL motif and functionally studied it with respect to the laminar inclination and grain size of rice
- OsBUL1 COMPLEX1 (OsBC1) is a basic HLH (bHLH) transcriptional activator that interacts with OsBUL1 only in the presence of LO9-177 forming a possible trimeric complex for cell elongation in the laminar joint of rice
- Three candidates, OsbHLH153, OsbHLH173 and OsbHLH174, quickly responded to BR and IAA involved in plant architecture except for OsbHLH173, whose expression level was too low to be detected; their overexpression in plants increased rice leaf angle
- OsbHLH98 Regulates Leaf Angle in Rice Through Transcriptional Repression of OsBUL1
- Our results demonstrate that OsbHLH98 negatively regulates leaf angle by counteracting brassinosteroid-induced cell elongation via the repression of OsBUL1 transcription
Connection
- APG~OsPIL16, PGL2~OsBUL1~OsbHLH173, An atypical bHLH protein encoded by POSITIVE REGULATOR OF GRAIN LENGTH 2 is involved in controlling grain length and weight of rice through interaction with a typical bHLH protein APG, PGL2 interacts with a typical bHLH protein APG, a negative regulator of rice grain length and weight, in vitro and in vivo
- APG~OsPIL16, PGL2~OsBUL1~OsbHLH173, An atypical bHLH protein encoded by POSITIVE REGULATOR OF GRAIN LENGTH 2 is involved in controlling grain length and weight of rice through interaction with a typical bHLH protein APG, These findings suggest that PGL2 and PGL1 redundantly suppress the function of APG by forming heterodimers to positively regulate the rice grain length, while the pathway through which BU1, the closest homolog of PGL2, controls grain length is independent of APG
- PGL1, PGL2~OsBUL1~OsbHLH173, An atypical bHLH protein encoded by POSITIVE REGULATOR OF GRAIN LENGTH 2 is involved in controlling grain length and weight of rice through interaction with a typical bHLH protein APG, These findings suggest that PGL2 and PGL1 redundantly suppress the function of APG by forming heterodimers to positively regulate the rice grain length, while the pathway through which BU1, the closest homolog of PGL2, controls grain length is independent of APG
- OsBDG1, PGL2~OsBUL1~OsbHLH173, Oryza sativa BRASSINOSTEROID UPREGULATED1 LIKE1 Induces the Expression of a Gene Encoding a Small Leucine-Rich-Repeat Protein to Positively Regulate Lamina Inclination and Grain Size in Rice., Here, we identified a putative downstream gene of OsBUL1, OsBUL1 DOWNSTREAM GENE1 (OsBDG1) encoding a small protein with short leucine-rich-repeats by cDNA microarray analyses in the lamina joint and panicles of wild-type and osbul1 plants
- OsBDG1, PGL2~OsBUL1~OsbHLH173, Oryza sativa BRASSINOSTEROID UPREGULATED1 LIKE1 Induces the Expression of a Gene Encoding a Small Leucine-Rich-Repeat Protein to Positively Regulate Lamina Inclination and Grain Size in Rice., Moreover, transgenic rice expressing OsBDG1 under the control of OsBUL1 promoter also shows enlarged leaf bending and grain size phenotypes
- BU1~OsbHLH174, PGL2~OsBUL1~OsbHLH173, Genome-wide association studies reveal that members of bHLH subfamily 16 share a conserved function in regulating flag leaf angle in rice Oryza sativa., Three candidates, OsbHLH153, OsbHLH173 and OsbHLH174, quickly responded to BR and IAA involved in plant architecture except for OsbHLH173, whose expression level was too low to be detected; their overexpression in plants increased rice leaf angle
- LO9-177, PGL2~OsBUL1~OsbHLH173, Rice Leaf Angle and Grain Size Are Affected by the OsBUL1 Transcriptional Activator Complex., To understand the molecular network of OsBUL1 function in rice, we isolated a novel OsBUL1-interacting protein, LO9-177, an uncharacterized protein containing a KxDL motif, and functionally studied it with respect to the lamina inclination and grain size of rice
- LO9-177, PGL2~OsBUL1~OsbHLH173, Rice Leaf Angle and Grain Size Are Affected by the OsBUL1 Transcriptional Activator Complex., OsBUL1 COMPLEX1 (OsBC1) is a basic helix-loop-helix (bHLH) transcriptional activator that interacts with OsBUL1 only in the presence of LO9-177 forming a possible trimeric complex for cell elongation in the lamina joint of rice
- LO9-177, PGL2~OsBUL1~OsbHLH173, Rice Leaf Angle and Grain Size Are Affected by the OsBUL1 Transcriptional Activator Complex., Together, these results demonstrated that a novel protein complex consisting of OsBUL1, LO9-177, and OsBC1 is associated with the HLH-bHLH system, providing new insight into the molecular functional network based on HLH-bHLH proteins for cell elongation
- OsbHLH98, PGL2~OsBUL1~OsbHLH173, OsbHLH98 regulates leaf angle in rice through transcriptional repression of OsBUL1., OsbHLH98 regulates leaf angle in rice through transcriptional repression of OsBUL1.
- OsbHLH98, PGL2~OsBUL1~OsbHLH173, OsbHLH98 regulates leaf angle in rice through transcriptional repression of OsBUL1., Furthermore, we established by chromatin immunoprecipitation and CUT&RUN that OsBUL1 is a direct downstream target of OsbHLH98
- OsbHLH98, PGL2~OsBUL1~OsbHLH173, OsbHLH98 regulates leaf angle in rice through transcriptional repression of OsBUL1., Transactivation assays and reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis indicated that OsbHLH98 represses OsBUL1 transcription
- OsbHLH98, PGL2~OsBUL1~OsbHLH173, OsbHLH98 regulates leaf angle in rice through transcriptional repression of OsBUL1., Our results demonstrate that OsbHLH98 negatively regulates leaf angle by counteracting brassinosteroid-induced cell elongation via the repression of OsBUL1 transcription
- HOS59, PGL2~OsBUL1~OsbHLH173, KNOX II transcription factor HOS59 functions in regulating rice grain size., The qRT-PCR results showed that mutation of the HOS59 gene led to upregulation of some grain size-related genes such as OsSPL13, OsSPL18, and PGL2

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