OsTB1,FC1,SCM3,MP3

2015-01-20 | Categories genes  | Tags culm  auxin  branching  cytokinin  growth  shoot  meristem  tiller  crown  shoot apical meristem  lamina  root  crown root  strigolactone  height  tillering  dwarf  transporter  stem  resistance  inflorescence  lodging  grain  grain yield  yield  tiller number  phosphorus  panicle  domestication  breeding  tiller bud outgrowth 

• Information
  • Symbol: OsTB1,FC1,SCM3,MP3
  • MSU: LOC_Os03g49880
  • RAPdb: Os03g0706500
• Publication
  • OsTIR1 and OsAFB2 downregulation via OsmiR393 overexpression leads to more tillers, early flowering and less tolerance to salt and drought in rice, 2012, PLoS One.
  • The OsTB1 gene negatively regulates lateral branching in rice, 2003, The Plant Journal.
  • DWARF10, an RMS1/MAX4/DAD1 ortholog, controls lateral bud outgrowth in rice, 2007, Plant J.
  • The interaction between OsMADS57 and OsTB1 modulates rice tillering via DWARF14, 2013, Nat Commun.
  • FINE CULM1 FC1 works downstream of strigolactones to inhibit the outgrowth of axillary buds in rice, 2010, Plant Cell Physiol.
  • Genome-wide binding analysis of the transcription activator ideal plant architecture1 reveals a complex network regulating rice plant architecture, 2013, Plant Cell.
  • Isolation of a novel lodging resistance QTL gene involved in strigolactone signaling and its pyramiding with a QTL gene involved in another mechanism, 2014, Mol Plant.
  • Production of novel beneficial alleles of a rice yield-related QTL by CRISPR/Cas9., 2020, Plant Biotechnol J.
  • MiR529a controls plant height, tiller number, panicle architecture and grain size by regulating SPL target genes in rice Oryza sativa L., 2021, Plant Sci.
  • In-frame mutation in rice TEOSINTE BRANCHED1 OsTB1 improves productivity under phosphorus deficiency., 2023, Plant Sci.
  • MORE PANICLES 3, a natural allele of OsTB1/FC1, impacts rice yield in paddy fields at elevated CO2 levels., 2023, Plant J.
• Genbank accession number
  • AB088343
• Key message
  • We also demonstrated that a rice strain carrying a classical morphological marker mutation, fine culm 1 (fc1), contain the loss-of-function mutation of OsTB1 and exhibits enhanced lateral branching
  • The expression of an auxin transporter (OsAUX1) and a tillering inhibitor (OsTB1) were downregulated by overexpression of OsmiR393, which suggested that a gene chain from OsmiR393 to rice tillering may be from OsTIR1 and OsAFB2 to OsAUX1, which affected the transportation of auxin, then to OsTB1, which finally controlled tillering
  • We propose that FC1 acts as an integrator of multiple signaling pathways and is essential to the fine-tuning of shoot branching in rice
  • On the other hand, the expression level of FC1 is negatively regulated by cytokinin treatment
  • Here we report that OsMADS57 interacts with OsTB1 (TEOSINTE BRANCHED1) and targets D14 (Dwarf14) to control the outgrowth of axillary buds in rice
  • Expression of OsTB1, as examined with a putative promoter-glucuronidase (GUS) gene fusion, was observed throughout the axillary bud, as well as the basal part of the shoot apical meristem, vascular tissues in the pith and the lamina joint
  • An in situ hybridization analysis showed that FC1 mRNA accumulates in axillary buds, the shoot apical meristem, young leaves, vascular tissues and the tips of crown roots
  • We found that the increased tillering phenotype of fine culm1 (fc1) mutants of rice is not rescued by the application of 1 microM GR24, a synthetic SL analog
  • Treatment with a high concentration of GR24 (10 microM) causes suppression of tiller growth in wild-type plants, but is not effective on fc1 mutants, implying that proper FC1 functioning is required for SLs to inhibit bud growth
  • Overexpression of FC1 partially rescued d3-2 defects in the tiller growth and plant height
  • The rice TB1 gene (OsTB1) was first identified based on its sequence similarity with maize TEOSINTE BRANCHED 1 (TB1), which is involved in lateral branching in maize
  • Transgenic rice plants overexpressing OsTB1 exhibited markedly reduced lateral branching without the propagation of axillary buds being affected
  • Taking these data together, we concluded that OsTB1 functions as a negative regulator for lateral branching in rice, presumably through expression in axillary buds
  • The OsTB1 gene negatively regulates lateral branching in rice
  • FINE CULM1 (FC1) works downstream of strigolactones to inhibit the outgrowth of axillary buds in rice
  • Therefore, OsMIR444a-regulated OsMADS57, together with OsTB1, target D14 to control tillering
  • The interaction between OsMADS57 and OsTB1 modulates rice tillering via DWARF14
  • In addition, we present observations that suggest that FINE CULM1 (FC1), a rice ortholog of teosinte branched 1 (tb1), possibly works independently of the branching inhibitor pathway
  • Our study of a locus involved in resistance to lodging demonstrated that saturation editing of the qSCSA3-1/OsTB1/SCM3 region led to loss-of-function, normal-like, and gain-of-function plant types, based on OsTB1 expression and stem cross-section area
  • One gain-of-function allele recapitulated a previously described beneficial allele of OsTB1 carrying a TGTG insertion in the 5’ region of OsTB1 and enhanced OsTB1 expression at the inflorescence formation stage
  • We propose that mildly enhanced tillering by in-frame mutation of OsTB1 can improve grain yield under low phosphorus conditions
  • In contrast, in-frame mutations in OsTB1 had the effect of slightly increasing tiller numbers, and the in-frame mutants had 40% more panicles than non-mutated plants
  • Our findings show that the moderately increased panicle number combined with large-sized panicles using MP3 could be a novel IPA and contribute to an increase in rice production under climate change with rising atmospheric CO(2) levels
  • MP3 is a natural allele of OsTB1/FC1, previously reported as a negative regulator of tiller bud outgrowth
  • No selective sweep at MP3 in either the temperate japonica or indica subgroups suggested that MP3 has not been involved and utilized in artificial selection during domestication or breeding
• Connection
  • OsAUX1, OsTB1~FC1~SCM3~MP3, OsTIR1 and OsAFB2 downregulation via OsmiR393 overexpression leads to more tillers, early flowering and less tolerance to salt and drought in rice, The expression of an auxin transporter (OsAUX1) and a tillering inhibitor (OsTB1) were downregulated by overexpression of OsmiR393, which suggested that a gene chain from OsmiR393 to rice tillering may be from OsTIR1 and OsAFB2 to OsAUX1, which affected the transportation of auxin, then to OsTB1, which finally controlled tillering
  • OsAFB2, OsTB1~FC1~SCM3~MP3, OsTIR1 and OsAFB2 downregulation via OsmiR393 overexpression leads to more tillers, early flowering and less tolerance to salt and drought in rice, The expression of an auxin transporter (OsAUX1) and a tillering inhibitor (OsTB1) were downregulated by overexpression of OsmiR393, which suggested that a gene chain from OsmiR393 to rice tillering may be from OsTIR1 and OsAFB2 to OsAUX1, which affected the transportation of auxin, then to OsTB1, which finally controlled tillering
  • OsTB1~FC1~SCM3~MP3, OsTIR1, OsTIR1 and OsAFB2 downregulation via OsmiR393 overexpression leads to more tillers, early flowering and less tolerance to salt and drought in rice, The expression of an auxin transporter (OsAUX1) and a tillering inhibitor (OsTB1) were downregulated by overexpression of OsmiR393, which suggested that a gene chain from OsmiR393 to rice tillering may be from OsTIR1 and OsAFB2 to OsAUX1, which affected the transportation of auxin, then to OsTB1, which finally controlled tillering
  • HTD2~D88~D14, OsTB1~FC1~SCM3~MP3, The interaction between OsMADS57 and OsTB1 modulates rice tillering via DWARF14, Here we report that OsMADS57 interacts with OsTB1 (TEOSINTE BRANCHED1) and targets D14 (Dwarf14) to control the outgrowth of axillary buds in rice
  • HTD2~D88~D14, OsTB1~FC1~SCM3~MP3, The interaction between OsMADS57 and OsTB1 modulates rice tillering via DWARF14, Interaction of OsMADS57 with OsTB1 reduced OsMADS57 inhibition of D14 transcription
  • HTD2~D88~D14, OsTB1~FC1~SCM3~MP3, The interaction between OsMADS57 and OsTB1 modulates rice tillering via DWARF14, Therefore, OsMIR444a-regulated OsMADS57, together with OsTB1, target D14 to control tillering
  • OsMADS57, OsTB1~FC1~SCM3~MP3, The interaction between OsMADS57 and OsTB1 modulates rice tillering via DWARF14, Here we report that OsMADS57 interacts with OsTB1 (TEOSINTE BRANCHED1) and targets D14 (Dwarf14) to control the outgrowth of axillary buds in rice
  • OsMADS57, OsTB1~FC1~SCM3~MP3, The interaction between OsMADS57 and OsTB1 modulates rice tillering via DWARF14, Interaction of OsMADS57 with OsTB1 reduced OsMADS57 inhibition of D14 transcription
  • OsMADS57, OsTB1~FC1~SCM3~MP3, The interaction between OsMADS57 and OsTB1 modulates rice tillering via DWARF14, Therefore, OsMIR444a-regulated OsMADS57, together with OsTB1, target D14 to control tillering
  • OsMADS57, OsTB1~FC1~SCM3~MP3, The interaction between OsMADS57 and OsTB1 modulates rice tillering via DWARF14, The interaction between OsMADS57 and OsTB1 modulates rice tillering via DWARF14
  • IAA6~OsIAA6, OsTB1~FC1~SCM3~MP3, OsIAA6, a member of the rice Aux/IAA gene family, is involved in drought tolerance and tiller outgrowth., A knock-down mutant of OsIAA6 showed abnormal tiller outgrowth, apparently due to the regulation of the auxin transporter OsPIN1 and the rice tillering inhibitor OsTB1
  • OsMADS57, OsTB1~FC1~SCM3~MP3, OsMADS57 together with OsTB1 coordinates transcription of its target OsWRKY94 and D14 to switch its organogenesis to defense for cold adaptation in rice., OsMADS57 together with OsTB1 coordinates transcription of its target OsWRKY94 and D14 to switch its organogenesis to defense for cold adaptation in rice.
  • OsMADS57, OsTB1~FC1~SCM3~MP3, OsMADS57 together with OsTB1 coordinates transcription of its target OsWRKY94 and D14 to switch its organogenesis to defense for cold adaptation in rice., Here, we demonstrate that interacting transcription factors OsMADS57 and OsTB1 directly target the defense gene OsWRKY94 and the organogenesis gene D14 to trade off the functions controlling/moderating rice tolerance to cold
  • OsMADS57, OsTB1~FC1~SCM3~MP3, OsMADS57 together with OsTB1 coordinates transcription of its target OsWRKY94 and D14 to switch its organogenesis to defense for cold adaptation in rice., We demonstrated that OsMADS57 and OsTB1 conversely affect rice chilling tolerance via targeting OsWRKY94
  • OsSHI1, OsTB1~FC1~SCM3~MP3, OsSHI1 Regulates Plant Architecture Through Modulating the Transcriptional Activity of IPA1 in Rice., Moreover, OsSHI1 could bind directly to the promoter regions of both OsTB1 and OsDEP1 through a novel cis-element (T/GCTCTAC motif)
  • OsSHI1, OsTB1~FC1~SCM3~MP3, OsSHI1 Regulates Plant Architecture Through Modulating the Transcriptional Activity of IPA1 in Rice., Further, OsSHI1 represses the transcriptional activation activity of IPA1 by affecting its DNA binding activity towards the promoters of both OsTB1 and OsDEP1, resulting in increased tiller number and diminished panicle size
  • OsTB1~FC1~SCM3~MP3, OsVIL2, Chromatin Interacting Factor OsVIL2 Is Required for Outgrowth of Axillary Buds in Rice., Analysis of the expression patterns of the tiller-related genes revealed that expression of OsTB1, which is a negative regulator of bud outgrowth, was increased in osvil2 mutants
  • OsTB1~FC1~SCM3~MP3, OsVIL2, Chromatin Interacting Factor OsVIL2 Is Required for Outgrowth of Axillary Buds in Rice., Chromatin immunoprecipitation assays showed that OsVIL2 binds to the promoter region of OsTB1 chromatin in wild-type rice, but the binding was not observed in osvil2 mutants
  • OsTB1~FC1~SCM3~MP3, OsVIL2, Chromatin Interacting Factor OsVIL2 Is Required for Outgrowth of Axillary Buds in Rice., Tiller number of double mutant osvil2 ostb1 was similar to that of ostb1 , suggesting that osvil2 is epistatic to ostb1
  • OsTB1~FC1~SCM3~MP3, OsVIL2, Chromatin Interacting Factor OsVIL2 Is Required for Outgrowth of Axillary Buds in Rice., These observations indicate that OsVIL2 suppresses OsTB1 expression by chromatin modification, thereby inducing bud outgrowth
  • OsTB1~FC1~SCM3~MP3, REP1~OsTb2, Neo-functionalization of a Teosinte branched 1 homologue mediates adaptations of upland rice., Here, we report that an OsTb1 duplicate gene (OsTb2) has been artificially selected during upland rice adaptation and that natural variation in OsTb2 is associated with tiller number
  • OsTB1~FC1~SCM3~MP3, REP1~OsTb2, Neo-functionalization of a Teosinte branched 1 homologue mediates adaptations of upland rice., Interestingly, transgenic rice overexpressing this gene shows increased rather than decreased tillering, suggesting that OsTb2 gains a regulatory effect opposite to that of OsTb1 following duplication
  • OsTB1~FC1~SCM3~MP3, REP1~OsTb2, Neo-functionalization of a Teosinte branched 1 homologue mediates adaptations of upland rice., Functional analyses suggest that the OsTb2 protein positively regulates tillering by interacting with the homologous OsTb1 protein and counteracts the inhibitory effect of OsTb1 on tillering
  • OsPIL11, OsTB1~FC1~SCM3~MP3, PIL transcription factors directly interact with SPLs and repress tillering/branching in plants., In rice, overexpression and loss-of-function mutations of OsPIL11 reduce or increase tiller number by regulating the expression of OsTB1

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