- Information
- Symbol: OsMYB80,BM1,OsMS188
- MSU: LOC_Os04g39470
- RAPdb: Os04g0470600
- Publication
- MYB80, a regulator of tapetal and pollen development, is functionally conserved in crops, 2012, Plant Mol Biol.
- MYB80 homologues in Arabidopsis, cotton and Brassica: regulation and functional conservation in tapetal and pollen development, 2014, BMC Plant Biol.
- OsMYB80 regulates anther development and pollen fertility by targeting multiple biological pathways., 2020, Plant Cell Physiol.
- The MYB transcription factor Baymax1 plays a critical role in rice male fertility, 2020, Theor Appl Genet.
- OsMS188 Is a Key Regulator of Tapetum Development and Sporopollenin Synthesis in Rice, 2021, Rice (N Y).
- OsMS188 Is a Key Regulator of Tapetum Development and Sporopollenin Synthesis in Rice, 2021, Rice (N Y).
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Genbank accession number
- Key message
- OsMYB80 regulates anther development and pollen fertility by targeting multiple biological pathways.
- To understand how OsMYB80 regulates anther development, RNA-seq analysis was conducted to identify genes differentially regulated by OsMYB80 in rice anthers
- The osmyb80 mutants exhibited premature tapetum cell death, lack of Ubisch bodies, no exine, and microspore degeneration
- Here we isolated two allelic male sterile mutants of OsMYB80 and investigated how OsMYB80 regulates male fertility in rice
- Functional annotation of the 188 genes suggested that OsMYB80 regulates male fertility by directly targeting multiple biological processes
- Moreover, BM1 affects the expression of several known genes related to tapetum and microspore development
- Collectively, our results suggest that BM1 is one of key regulators for rice male fertility and may serve as a potential target for rice male-sterile line breeding and hybrid seed production
- The bm1 mutant exhibited slightly lagging meiosis, aborted transition of the tapetum to a secretory type, premature tapetal degeneration, and abnormal pollen exine formation, leading to ultimately lacks of visible pollens in the mature white anthers
- Map-based cloning, complementation and targeted mutagenesis using CRISPR/Cas9 technology demonstrated that the mutated LOC_Os04g39470 is the causal gene in bm1
- Results: In this study, we obtained several lines in which the MYB transcription factor OsMS188 was knocked out through the CRISPR-Cas9 approach
- OsMS188 Is a Key Regulator of Tapetum Development and Sporopollenin Synthesis in Rice
- The osms188 lines exhibited a male-sterile phenotype with aberrant development and degeneration of tapetal cells, absence of the sexine layer and defective anther cuticles
- Conclusion: Overall, OsMS188 is a key regulator of tapetum development and pollen wall formation
- Connection
- DTD~EAT1, OsMYB80~BM1~OsMS188, The MYB transcription factor Baymax1 plays a critical role in rice male fertility, BM1 can physically interacts with bHLH protein TIP2, EAT1, and PHD (plant homeodomain)-finger member TIP3, respectively
- OsMYB80~BM1~OsMS188, TIP2~bHLH142, The MYB transcription factor Baymax1 plays a critical role in rice male fertility, BM1 can physically interacts with bHLH protein TIP2, EAT1, and PHD (plant homeodomain)-finger member TIP3, respectively
- OsMYB80~BM1~OsMS188, TIP3, The MYB transcription factor Baymax1 plays a critical role in rice male fertility, BM1 can physically interacts with bHLH protein TIP2, EAT1, and PHD (plant homeodomain)-finger member TIP3, respectively
- OsMYB80~BM1~OsMS188, TDR, OsMS188 Is a Key Regulator of Tapetum Development and Sporopollenin Synthesis in Rice, Electrophoretic mobility shift assays (EMSAs) and activation assays revealed that TDR directly regulates OsMS188 expression
- OsMYB80~BM1~OsMS188, TDR, OsMS188 Is a Key Regulator of Tapetum Development and Sporopollenin Synthesis in Rice, Additionally, protein interaction assays indicated that TDR interacts with OsMS188 to regulate downstream gene expression
- OsMYB80~BM1~OsMS188, OsTMS18, Mutation of glucose-methanol-choline oxidoreductase leads to thermosensitive genic male sterility in rice and Arabidopsis., Further analysis showed that the tapetal transcription factor OsMS188 directly regulates OsTMS18 for pollen wall formation
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