DAO

• Symbol: DAO
• MSU: LOC_Os04g39980
• RAPdb: Os04g0475600

• A role for a dioxygenase in auxin metabolism and reproductive development in rice, 2013, Dev Cell.

Genbank accession number

• Here, we demonstrate that the dioxygenase for auxin oxidation (DAO) gene, encoding a putative 2-oxoglutarate-dependent-Fe (II) dioxygenase, is essential for anther dehiscence, pollen fertility, and seed initiation in rice
• Furthermore, exogenous application of IAA or overexpression of the auxin biosynthesis gene OsYUCCA1 phenocopies the dao mutants
• Collectively, these data support a key role of DAO in auxin catabolism and maintenance of auxin homeostasis central to plant reproductive development
• Rice mutant lines lacking a functional DAO display increased levels of free IAA in anthers and ovaries
• We show that recombinant DAO converts the active IAA into biologically inactive 2-oxoindole-3-acetic acid (OxIAA) in vitro