OsAMT1;1,OsAMT1.1

2015-01-20 | Categories genes  | Tags transporter  nitrogen  growth  root  grain filling  domestication  yield  seed  grain  grain yield  shoot  Kinase  protein phosphatase  homeostasis  plasma membrane 

• Information
  • Symbol: OsAMT1;1,OsAMT1.1
  • MSU: LOC_Os04g43070
  • RAPdb: Os04g0509600
• Publication
  • Diversity and selective sweep in the OsAMT1;1 genomic region of rice, 2011, BMC Evol Biol.
  • Over-expression of the rice OsAMT1-1 gene increases ammonium uptake and content, but impairs growth and development of plants under high ammonium nutrition, 2006, Functional Plant Biology.
  • Influence of different nitrogen inputs on the members of ammonium transporter and glutamine synthetase genes in two rice genotypes having differential responsiveness to nitrogen, 2012, Mol Biol Rep.
  • AMT1;1 transgenic rice plants with enhanced NH4+ permeability show superior growth and higher yield under optimal and suboptimal NH4+ conditions, 2014, J Exp Bot.
  • Differential expression of three members of the AMT1 gene family encoding putative high-affinity NH4+ transporters in roots of Oryza sativa subspecies indica, 2003, Plant Cell Environ.
  • Feedback regulation of the ammonium transporter gene family AMT1 by glutamine in rice, 2003, Plant Cell Physiol.
  • Distinct expression and function of three ammonium transporter genes OsAMT1;1-1;3 in rice, 2003, Plant Cell Physiol.
  • The rice OsAMT1;1 is a proton-independent feedback regulated ammonium transporter., 2014, Plant Cell Rep.
  • The OsAMT1.1 gene functions in ammonium uptake and ammonium-potassium homeostasis over low and high ammonium concentration ranges., 2016, J Genet Genomics.
  • Three polarly localized ammonium transporter 1 members are cooperatively responsible for ammonium uptake in rice under low ammonium condition, 2021, New Phytol.
  • Ammonium transporter 1 increases rice resistance to sheath blight by promoting nitrogen assimilation and ethylene signalling., 2022, Plant Biotechnol J.
  • OsAMT1;1 and OsAMT1;2 Coordinate Root Morphological and Physiological Responses to Ammonium for Efficient Nitrogen Foraging in Rice., 2022, Plant Cell Physiol.
  • Mutation of phytochrome B promotes resistance to sheath blight and saline-alkaline stress via increasing ammonium uptake in rice., 2022, Plant J.
• Genbank accession number
  • AAL05612
  • AF289477
  • AAL05612
  • AF289477
• Key message
  • The NH4(+) uptake of roots is mainly governed by membrane transporters, with OsAMT1;1 being a prominent member of the OsAMT1 gene family that is known to be involved in NH4(+) transport in rice plants
  • A transgenic approach was undertaken to investigate the role of a rice ammonium transporter (OsAMT1-1) in ammonium uptake and consequent ammonium assimilation under different nitrogen regimes
  • OsAMT1;1 also enhanced overall plant growth, especially under suboptimal NH4(+) levels
  • These results suggest that OsAMT1;1 has the potential for improving nitrogen use efficiency, plant growth, and grain yield under both suboptimal and optimal nitrogen fertilizer conditions
  • Gene expression analysis of members of ammonium transporter gene family in flag leaves during active grain filling stage revealed that all the three members of OsAMT3 family genes (OsAMT1;1-3), only one member of OsAMT2 family i
  • To study the regulation of ammonium uptake into rice roots, three ammonium transporter genes (OsAMT1;1, 1;2 and 1;3; Oryza sativa ammonium transporter) were isolated and examined
  • Northern blot analysis showed a distinct expression pattern for the three genes; more constitutive expression in shoots and roots for OsAMT1;1, root-specific and ammonium-inducible expression for OsAMT1;2, and root-specific and nitrogen-derepressible expression for OsAMT1;3
  • In both the genotypes, both increase and decline in seed protein contents matched with the expressions levels of OsAMT1;1, OsGS1;1 and OsGS1;2 in the flag leaves during grain filling stage indicating that high nitrogen nutrition in KN3119 probably causes the repression of these genes which might be important during grain filling
  • CONCLUSIONS: The severe reduction in nucleotide variation at OsAMT1;1 in rice was caused by a selective sweep around OsAMT1;1, which may reflect the nitrogen uptake system under strong selection by the paddy soil during the domestication of rice
  • OsAMT1;1 is a high-affinity ammonium transporter in rice (Oryza sativa L
  • rufipogon on either side of the promoter of OsAMT1;1, demonstrating a strong natural selection within or nearby the ammonium transporter
  • The three members of the rice OsAMT1 gene family of ammonium transporters show distinct expression patterns; constitutive and ammonium-promoted expression in shoots and roots for OsAMT1;1; root-specific and ammonium-inducible expression for OsAMT1;2; root-specific and nitrogen-repressible expression for OsAMT1;3 [Sonoda et al
  • This study shows that OsAMT1;1 is a constitutively expressed, nitrogen-responsive gene, and its protein product is localized in the plasma membrane
  • , OsAMT2;3 and the high affinity OsAMT1;1 were differentially expressed and were affected by different doses of nitrogen
  • Distinct expression and function of three ammonium transporter genes (OsAMT1;1-1;3) in rice
  • Transgenic rice lines (L-2 and L-3) overexpressing the OsAMT1;1 gene had the same root structure as the wild type (WT)
  • Ammonium application to roots following a period of nitrogen starvation induced accumulation of OsAMT1;1 and OsAMT1;2 mRNA, but a decrease of OsAMT1;3 mRNA levels
  • The expression patterns of the three genes showed good correlation (positive in OsAMT1;1 and OsAMT1;2, negative in OsAMT1;3) with the root tissue contents of glutamine but not of ammonium
  • Over-expression of the rice OsAMT1-1 gene increases ammonium uptake and content, but impairs growth and development of plants under high ammonium nutrition
  • Inhibition of yeast growth or currents elicited from oocytes by ammonium assimilation inhibitor L-methionine sulfoximine indicates that NH4 (+) transport by OsAMT1;1 is likely feedback regulated by accumulation of the substrate
  • Our results show that OsAMT1;1 is a NH4 (+) transporter with relatively lower affinity to NH4 (+) (110-129 μM in oocytes and yeast cells, respectively)
  • In addition, effects of phosphorylation inhibitors imply that NH4 (+) uptake by OsAMT1;1 is also modulated by tyrosine-specific protein kinase or calcium-regulated serine/threonine-specific protein phosphatase involved phosphorylation processes
  • The OsAMT1.1 gene functions in ammonium uptake and ammonium-potassium homeostasis over low and high ammonium concentration ranges.
  • Spatial expression analysis showed that the upregulated expression of OsAMT1;1 and OsAMT1;2 and downregulated expression of OsAMT1;3 by ammonium were higher in the root mature region than in the root tips
  • Upon exposure to ammonium, localization of OsAMT1;1 and OsAMT1;2 was also observed in the endoplasmic reticulum, but their abundance in the plasma membrane was not changed
  • OsAMT1;1 and OsAMT1;2 Coordinate Root Morphological and Physiological Responses to Ammonium for Efficient Nitrogen Foraging in Rice.
  • The two independent double mutants (dko) defective in OsAMT1;1 and OsAMT1;2 failed to induce NH4+ uptake and stimulate LR formation, suggesting that OsAMT1s conferred the substrate-dependent root NH4+ foraging
• Connection
  • OsAMT1;1~OsAMT1.1, OsGS~OsGS1~GS1~OsGS1;1~OsGLN1;1, Influence of different nitrogen inputs on the members of ammonium transporter and glutamine synthetase genes in two rice genotypes having differential responsiveness to nitrogen, In both the genotypes, both increase and decline in seed protein contents matched with the expressions levels of OsAMT1;1, OsGS1;1 and OsGS1;2 in the flag leaves during grain filling stage indicating that high nitrogen nutrition in KN3119 probably causes the repression of these genes which might be important during grain filling
  • GLN1;2~GS1;2~OsGS1;2, OsAMT1;1~OsAMT1.1, Influence of different nitrogen inputs on the members of ammonium transporter and glutamine synthetase genes in two rice genotypes having differential responsiveness to nitrogen, In both the genotypes, both increase and decline in seed protein contents matched with the expressions levels of OsAMT1;1, OsGS1;1 and OsGS1;2 in the flag leaves during grain filling stage indicating that high nitrogen nutrition in KN3119 probably causes the repression of these genes which might be important during grain filling
  • OsAMT1;1~OsAMT1.1, OsAMT2;3~OsAMT2.3, Influence of different nitrogen inputs on the members of ammonium transporter and glutamine synthetase genes in two rice genotypes having differential responsiveness to nitrogen, , OsAMT2;3 and the high affinity OsAMT1;1 were differentially expressed and were affected by different doses of nitrogen
  • OsAMT1;1~OsAMT1.1, OsAMT1;2~OsAMT1.2, Feedback regulation of the ammonium transporter gene family AMT1 by glutamine in rice, The three members of the rice OsAMT1 gene family of ammonium transporters show distinct expression patterns; constitutive and ammonium-promoted expression in shoots and roots for OsAMT1;1; root-specific and ammonium-inducible expression for OsAMT1;2; root-specific and nitrogen-repressible expression for OsAMT1;3 [Sonoda et al
  • OsAMT1;1~OsAMT1.1, OsAMT1;2~OsAMT1.2, Feedback regulation of the ammonium transporter gene family AMT1 by glutamine in rice, Ammonium application to roots following a period of nitrogen starvation induced accumulation of OsAMT1;1 and OsAMT1;2 mRNA, but a decrease of OsAMT1;3 mRNA levels
  • OsAMT1;1~OsAMT1.1, OsAMT1;2~OsAMT1.2, Feedback regulation of the ammonium transporter gene family AMT1 by glutamine in rice, The expression patterns of the three genes showed good correlation (positive in OsAMT1;1 and OsAMT1;2, negative in OsAMT1;3) with the root tissue contents of glutamine but not of ammonium
  • OsAMT1;1~OsAMT1.1, OsAMT1;3~OsAMT1.3, Feedback regulation of the ammonium transporter gene family AMT1 by glutamine in rice, The three members of the rice OsAMT1 gene family of ammonium transporters show distinct expression patterns; constitutive and ammonium-promoted expression in shoots and roots for OsAMT1;1; root-specific and ammonium-inducible expression for OsAMT1;2; root-specific and nitrogen-repressible expression for OsAMT1;3 [Sonoda et al
  • OsAMT1;1~OsAMT1.1, OsAMT1;3~OsAMT1.3, Feedback regulation of the ammonium transporter gene family AMT1 by glutamine in rice, Ammonium application to roots following a period of nitrogen starvation induced accumulation of OsAMT1;1 and OsAMT1;2 mRNA, but a decrease of OsAMT1;3 mRNA levels
  • OsAMT1;1~OsAMT1.1, OsAMT1;3~OsAMT1.3, Feedback regulation of the ammonium transporter gene family AMT1 by glutamine in rice, The expression patterns of the three genes showed good correlation (positive in OsAMT1;1 and OsAMT1;2, negative in OsAMT1;3) with the root tissue contents of glutamine but not of ammonium
  • OsAMT1;1~OsAMT1.1, OsAMT1;2~OsAMT1.2, Distinct expression and function of three ammonium transporter genes OsAMT1;1-1;3 in rice, Northern blot analysis showed a distinct expression pattern for the three genes; more constitutive expression in shoots and roots for OsAMT1;1, root-specific and ammonium-inducible expression for OsAMT1;2, and root-specific and nitrogen-derepressible expression for OsAMT1;3
  • OsAMT1;1~OsAMT1.1, OsAMT1;3~OsAMT1.3, Distinct expression and function of three ammonium transporter genes OsAMT1;1-1;3 in rice, Northern blot analysis showed a distinct expression pattern for the three genes; more constitutive expression in shoots and roots for OsAMT1;1, root-specific and ammonium-inducible expression for OsAMT1;2, and root-specific and nitrogen-derepressible expression for OsAMT1;3
  • OsAMT1;1~OsAMT1.1, OsPTR6~PTR6~OsNPF7.3, Over-expression of OsPTR6 in rice increased plant growth at different nitrogen supplies but decreased nitrogen use efficiency at high ammonium supply, The expression of OsATM1 genes in over-expression lines showed that the OsPTR6 over expression increased OsAMT1.1, OsATM1.2 and OsAMT1.3 expression at 0.2 and 5.0mmol/L NH4(+) and 2.5mmol/L NH4NO3
  • OsAMT1;1~OsAMT1.1, OsDOF18, Transcription Factor OsDOF18 Controls Ammonium Uptake by Inducing Ammonium Transporters in Rice Roots., Among the 10 ammonium transporter genes examined here, expression of OsAMT1;1,<U+00A0>OsAMT1;3, OsAMT2;1, and OsAMT4;1 was reduced in osdof18 mutants, demonstrating that the ammonium transporter genes function downstream of OsDOF18
  • OsACTPK1, OsAMT1;1~OsAMT1.1, Lack of ACTPK1, an STY kinase, enhances ammonium uptake and use and promotes growth of rice seedlings under sufficient external ammonium., Root cellular expression of OsACTPK1 also overlapped with that of ammonium-induced OsAMT1;1 and OsAMT1;2
  • OsAMT1;1~OsAMT1.1, OsAMT1;2~OsAMT1.2, Three polarly localized ammonium transporter 1 members are cooperatively responsible for ammonium uptake in rice under low ammonium condition, Spatial expression analysis showed that the upregulated expression of OsAMT1;1 and OsAMT1;2 and downregulated expression of OsAMT1;3 by ammonium were higher in the root mature region than in the root tips
  • OsAMT1;1~OsAMT1.1, OsAMT1;2~OsAMT1.2, Three polarly localized ammonium transporter 1 members are cooperatively responsible for ammonium uptake in rice under low ammonium condition, Upon exposure to ammonium, localization of OsAMT1;1 and OsAMT1;2 was also observed in the endoplasmic reticulum, but their abundance in the plasma membrane was not changed
  • OsAMT1;1~OsAMT1.1, OsAMT1;3~OsAMT1.3, Three polarly localized ammonium transporter 1 members are cooperatively responsible for ammonium uptake in rice under low ammonium condition, Spatial expression analysis showed that the upregulated expression of OsAMT1;1 and OsAMT1;2 and downregulated expression of OsAMT1;3 by ammonium were higher in the root mature region than in the root tips
  • OsAMT1;1~OsAMT1.1, OsAMT1;2~OsAMT1.2, OsAMT1;1 and OsAMT1;2 Coordinate Root Morphological and Physiological Responses to Ammonium for Efficient Nitrogen Foraging in Rice., OsAMT1;1 and OsAMT1;2 Coordinate Root Morphological and Physiological Responses to Ammonium for Efficient Nitrogen Foraging in Rice.
  • OsAMT1;1~OsAMT1.1, OsAMT1;2~OsAMT1.2, OsAMT1;1 and OsAMT1;2 Coordinate Root Morphological and Physiological Responses to Ammonium for Efficient Nitrogen Foraging in Rice., These synergistic physiological and morphological responses were closely related to NH4+-induced expression of NH4+ transporters OsAMT1;1 and OsAMT1;2 in roots
  • OsAMT1;1~OsAMT1.1, OsAMT1;2~OsAMT1.2, OsAMT1;1 and OsAMT1;2 Coordinate Root Morphological and Physiological Responses to Ammonium for Efficient Nitrogen Foraging in Rice., The two independent double mutants (dko) defective in OsAMT1;1 and OsAMT1;2 failed to induce NH4+ uptake and stimulate LR formation, suggesting that OsAMT1s conferred the substrate-dependent root NH4+ foraging

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