MOC3,OsWUS,TAB1,OsTAB1

2015-01-20 | Categories genes  | Tags tiller  cytokinin  cytokinin response  development  map-based cloning  transcription factor  fertility  stem  spikelet  vegetative  floral  meristem  inflorescence  floral organ  floral organ number  axillary meristem  growth  auxin  panicle  culm  auxin response  plant growth  panicle development  plant stature 

• Information
  • Symbol: MOC3,OsWUS,TAB1,OsTAB1
  • MSU: LOC_Os04g56780
  • RAPdb: Os04g0663600
• Publication
  • MONOCULM 3, an Ortholog of WUSCHEL in Rice, Is Required for Tiller Bud Formation., 2015, J Genet Genomics.
  • Axillary Meristem Formation in Rice Requires the WUSCHEL Ortholog TILLERS ABSENT1., 2015, Plant Cell.
  • Homeobox Is Pivotal for OsWUS Controlling Tiller Development and Female Fertility in Rice., 2016, G3 (Bethesda).
  • TILLERS ABSENT1, the WUSCHEL ortholog, is not involved in stem cell maintenance in the shoot apical meristem in rice., 2019, Plant Signal Behav.
  • Antagonistic action of TILLERS ABSENT1 and FLORAL ORGAN NUMBER2 regulates stem cell maintenance during axillary meristem development in rice., 2019, New Phytol.
  • OsWUS promotes tiller bud growth by establishing weak apical dominance in rice, 2020, Plant Journal.
  • Changes in the expression pattern of OsWUS negatively regulate plant stature and panicle development in rice., 2023, G3 (Bethesda).
• Genbank accession number
  • AB218894
• Key message
  • Morphological observation revealed that the formation of tiller buds was disrupted in moc3
  • The expression of MOC3 was induced by cytokinins and defection of MOC3 affected the expression of several two-component cytokinin response regulators, OsRRs and ORRs
  • Homeobox Is Pivotal for OsWUS Controlling Tiller Development and Female Fertility in Rice.
  • OsWUS has recently been shown to be a transcription factor gene critical for tiller development and fertility in rice
  • Map-based cloning revealed that the mutant phenotypes were caused by a mutation in OsWUS Compared with the two previously reported null allelic mutants of OsWUS (tab1-1 and moc3-1), which could produce partial N-terminal peptides of OsWUS, the srt1 protein contained a deletion of only seven amino acids within the conserved homeobox domain of OsWUS
  • Recently, we showed that a double mutant of FLORAL ORGAN NUMBER2 (FON2) and ABERRANT SPIKELET AND PANICLE1 (ASP1) led to a marked enlargement of the inflorescence meristem, and that the TAB1 function is not associated with massive stem cells in this meristem
  • In addition, misexpression of TAB1 under the promoter of FON1 led to a slight reduction of the SAM size in wild type, suggesting that TAB1 is not a positive regulator of stem cells
  • In this paper, we confirmed that TAB1 is also unrelated to the enlargement of the SAM in the vegetative phase of the fon2 and fon2 asp1 mutants
  • Taking together, TAB1 seems not to be involved in meristem maintenance, irrespective of the meristem type
  • We revealed that stem cells were established at an early stage of axillary meristem development in wild type, but were not maintained in tab1
  • By contrast, the stem cell region and TAB1 expression domain were expanded in fon2, and FON2 overexpression inhibited axillary meristem formation
  • These results indicate that TAB1 is required to maintain stem cells during axillary meristem development, whereas FON2 negatively regulates stem cell fate by restricting TAB1 expression
  • OsWUS promotes tiller bud growth by establishing weak apical dominance in rice
  • Auxin response is enhanced in the dc1 mutant, and knocking out the auxin action-associated gene ABERRANT SPIKELET AND PANICLE 1 (ASP1) de-repressed growth of the tiller buds in the dc1 mutant, suggesting that OsWUS and ASP1 are both involved in outgrowth of the rice tiller bud
  • Collectively, these findings reveal an important role of OsWUS in tiller bud growth by influencing apical dominance, and provide the basis for an improved understanding of tiller bud development in rice
  • In this study, we identified and functionally characterized a low-tillering mutant decreased culm number 1 (dc1) that resulted from loss-of-function of OsWUS
  • Our results suggest that OsWUS is a precise regulatory element, its specific spatio-temporal expression pattern is critical for its function, and both loss-of-function and gain-of-function mutations lead to abnormal plant growth
  • Changes in the expression pattern of OsWUS negatively regulate plant stature and panicle development in rice.
  • OsWUS have distinct functions in meristem development with slightly tuned expression
• Connection
  • MOC1, MOC3~OsWUS~TAB1~OsTAB1, Tiller Bud Formation Regulators MOC3 and MOC1 Cooperatively Promote Tiller Bud Outgrowth by Activating FON1 Expression in Rice., Tiller Bud Formation Regulators MOC3 and MOC1 Cooperatively Promote Tiller Bud Outgrowth by Activating FON1 Expression in Rice.
  • MOC1, MOC3~OsWUS~TAB1~OsTAB1, Tiller Bud Formation Regulators MOC3 and MOC1 Cooperatively Promote Tiller Bud Outgrowth by Activating FON1 Expression in Rice., MOC1 functions as a co-activator of MOC3, which did not directly bind to the FON1 promoter, but could further activate the FON1 expression in the presence of MOC3
  • MOC1, MOC3~OsWUS~TAB1~OsTAB1, Tiller Bud Formation Regulators MOC3 and MOC1 Cooperatively Promote Tiller Bud Outgrowth by Activating FON1 Expression in Rice., Accordingly, FON1 is highly expressed at axillary meristems and shows remarkable decreased expression levels in moc1 and moc3 mutants
  • MOC1, MOC3~OsWUS~TAB1~OsTAB1, Tiller Bud Formation Regulators MOC3 and MOC1 Cooperatively Promote Tiller Bud Outgrowth by Activating FON1 Expression in Rice., Collectively, these results shed lights on the joint transcriptional regulation by MOC1 and MOC3, and establish a new framework for the control of tiller bud formation and outgrowth
  • FON1, MOC3~OsWUS~TAB1~OsTAB1, TILLERS ABSENT1, the WUSCHEL ortholog, is not involved in stem cell maintenance in the shoot apical meristem in rice., In addition, misexpression of TAB1 under the promoter of FON1 led to a slight reduction of the SAM size in wild type, suggesting that TAB1 is not a positive regulator of stem cells
  • FON2~FON4~TG1, MOC3~OsWUS~TAB1~OsTAB1, TILLERS ABSENT1, the WUSCHEL ortholog, is not involved in stem cell maintenance in the shoot apical meristem in rice., Recently, we showed that a double mutant of FLORAL ORGAN NUMBER2 (FON2) and ABERRANT SPIKELET AND PANICLE1 (ASP1) led to a marked enlargement of the inflorescence meristem, and that the TAB1 function is not associated with massive stem cells in this meristem
  • FON2~FON4~TG1, MOC3~OsWUS~TAB1~OsTAB1, TILLERS ABSENT1, the WUSCHEL ortholog, is not involved in stem cell maintenance in the shoot apical meristem in rice., In this paper, we confirmed that TAB1 is also unrelated to the enlargement of the SAM in the vegetative phase of the fon2 and fon2 asp1 mutants
  • MOC3~OsWUS~TAB1~OsTAB1, OsTPL~ASP1~OsLIS-L1~ASP-LSL, TILLERS ABSENT1, the WUSCHEL ortholog, is not involved in stem cell maintenance in the shoot apical meristem in rice., Recently, we showed that a double mutant of FLORAL ORGAN NUMBER2 (FON2) and ABERRANT SPIKELET AND PANICLE1 (ASP1) led to a marked enlargement of the inflorescence meristem, and that the TAB1 function is not associated with massive stem cells in this meristem
  • MOC3~OsWUS~TAB1~OsTAB1, OsTPL~ASP1~OsLIS-L1~ASP-LSL, TILLERS ABSENT1, the WUSCHEL ortholog, is not involved in stem cell maintenance in the shoot apical meristem in rice., In this paper, we confirmed that TAB1 is also unrelated to the enlargement of the SAM in the vegetative phase of the fon2 and fon2 asp1 mutants
  • MOC3~OsWUS~TAB1~OsTAB1, SaM, TILLERS ABSENT1, the WUSCHEL ortholog, is not involved in stem cell maintenance in the shoot apical meristem in rice., We previously showed that, in rice (Oryza sativa), the WUS ortholog TILLERS ABSENT1 (TAB1, also known as OsWUS) has no function in SAM maintenance, whereas it plays a crucial role in axillary meristem development
  • MOC3~OsWUS~TAB1~OsTAB1, SaM, TILLERS ABSENT1, the WUSCHEL ortholog, is not involved in stem cell maintenance in the shoot apical meristem in rice., In this paper, we confirmed that TAB1 is also unrelated to the enlargement of the SAM in the vegetative phase of the fon2 and fon2 asp1 mutants
  • MOC3~OsWUS~TAB1~OsTAB1, SaM, TILLERS ABSENT1, the WUSCHEL ortholog, is not involved in stem cell maintenance in the shoot apical meristem in rice., In addition, misexpression of TAB1 under the promoter of FON1 led to a slight reduction of the SAM size in wild type, suggesting that TAB1 is not a positive regulator of stem cells
  • FON2~FON4~TG1, MOC3~OsWUS~TAB1~OsTAB1, Antagonistic action of TILLERS ABSENT1 and FLORAL ORGAN NUMBER2 regulates stem cell maintenance during axillary meristem development in rice., To elucidate the genetic mechanisms underlying axillary meristem development in rice (Oryza sativa), we undertook a molecular genetic analysis focusing on TILLERS ABSENT1 (TAB1) and FLORAL ORGAN NUMBER2 (FON2), respective orthologs of the WUSCHEL and CLAVATA3 genes involved in SAM maintenance in Arabidopsis (Arabidopsis thaliana)
  • FON2~FON4~TG1, MOC3~OsWUS~TAB1~OsTAB1, Antagonistic action of TILLERS ABSENT1 and FLORAL ORGAN NUMBER2 regulates stem cell maintenance during axillary meristem development in rice., By contrast, the stem cell region and TAB1 expression domain were expanded in fon2, and FON2 overexpression inhibited axillary meristem formation
  • FON2~FON4~TG1, MOC3~OsWUS~TAB1~OsTAB1, Antagonistic action of TILLERS ABSENT1 and FLORAL ORGAN NUMBER2 regulates stem cell maintenance during axillary meristem development in rice., These results indicate that TAB1 is required to maintain stem cells during axillary meristem development, whereas FON2 negatively regulates stem cell fate by restricting TAB1 expression
  • MOC3~OsWUS~TAB1~OsTAB1, SaM, Antagonistic action of TILLERS ABSENT1 and FLORAL ORGAN NUMBER2 regulates stem cell maintenance during axillary meristem development in rice., To elucidate the genetic mechanisms underlying axillary meristem development in rice (Oryza sativa), we undertook a molecular genetic analysis focusing on TILLERS ABSENT1 (TAB1) and FLORAL ORGAN NUMBER2 (FON2), respective orthologs of the WUSCHEL and CLAVATA3 genes involved in SAM maintenance in Arabidopsis (Arabidopsis thaliana)
  • MOC3~OsWUS~TAB1~OsTAB1, OsTPL~ASP1~OsLIS-L1~ASP-LSL, OsWUS promotes tiller bud growth by establishing weak apical dominance in rice, Auxin response is enhanced in the dc1 mutant, and knocking out the auxin action-associated gene ABERRANT SPIKELET AND PANICLE 1 (ASP1) de-repressed growth of the tiller buds in the dc1 mutant, suggesting that OsWUS and ASP1 are both involved in outgrowth of the rice tiller bud

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