- Information
- Symbol: OsMT2b
- MSU: LOC_Os05g02070
- RAPdb: Os05g0111300
- Publication
- The promoter and the 5’-untranslated region of rice metallothionein OsMT2b gene are capable of directing high-level gene expression in germinated rice embryos, 2014, Plant Cell Rep.
- Down-regulation of metallothionein, a reactive oxygen scavenger, by the small GTPase OsRac1 in rice, 2004, Plant Physiol.
- Characteristic and expression analysis of a metallothionein gene, OsMT2b, down-regulated by cytokinin suggests functions in root development and seed embryo germination of rice, 2008, Plant Physiol.
- Identification of sugar response complex in the metallothionein OsMT2b gene promoter for enhancement of foreign protein production in transgenic rice., 2019, Plant Cell Rep.
- Genbank accession number
- Key message
- In contrast with wild-type plants, OsMT2b-RNA interference (RNAi) transgenic plants had serious handicap in plant growth and root formation, whereas OsMT2b-overexpressing transformants were dwarfed and presented more adventitious roots and big lateral roots
- Taken together, these results indicate a possible feedback regulation mechanism of OsMT2b to the level of endogenous cytokinins that is involved in root development and seed embryo germination of rice
- Characteristic and expression analysis of a metallothionein gene, OsMT2b, down-regulated by cytokinin suggests functions in root development and seed embryo germination of rice
- Transgenic plants overexpressing OsMT2b showed increased susceptibility to bacterial blight and blast fungus
- Histochemical staining analysis of transgenic rice carrying OsMT2b::GUS chimeric gene showed that high-level GUS activity was detected in germinated embryos and at the meristematic part of other tissues during germination
- OsMT2b was preferentially expressed in rice immature panicles, scutellum of germinating embryos, and primordium of lateral roots
- A rice subfamily p2 (type 2) MT gene, OsMT2b, has been shown previously to exhibit the most abundant gene expression in young rice seedling
- We found that the OsMT2b gene is strongly and differentially expressed in germinated rice embryos during seed germination and seedling development
- We found that the expression of a metallothionein gene (OsMT2b) was synergically down-regulated by OsRac1 and rice (Oryza sativa) blast-derived elicitors
- Here, we characterized rice (Oryza sativa) METALLOTHIONEIN2b (OsMT2b) molecularly and found that its expression was down-regulated by cytokinins
- Expression of OsMT2b was found to be regulated negatively by ethylene and hydrogen peroxide in rice stem node under flooding stress, but little is known about its response to sugar depletion
- Identification of sugar response complex in the metallothionein OsMT2b gene promoter for enhancement of foreign protein production in transgenic rice.
- A 146-bp sugar response complex MTSRC is identified in the promoter of rice metallothionein OsMT2b gene conferring high-level expression of luciferase reporter gene and bioactive recombinant haFGF in transgenic rice
- We found that the expression of OsMT2b gene is induced by sugar starvation in both rice suspension cells and germinated embryos
- Deletion analysis and functional assay of the OsMT2b promoter revealed that the 5’-flanking region of the OsMT2b between nucleotides -<U+2009>351 and -<U+2009>121, which contains the sugar response complex (-<U+2009>266 to -<U+2009>121, designated MTSRC) is responsible for high-level promoter activity under sugar starvation
- Connection
- OsMT2b, OsRac1, Down-regulation of metallothionein, a reactive oxygen scavenger, by the small GTPase OsRac1 in rice, We found that the expression of a metallothionein gene (OsMT2b) was synergically down-regulated by OsRac1 and rice (Oryza sativa) blast-derived elicitors
- OsMT2b, OsRac1, Down-regulation of metallothionein, a reactive oxygen scavenger, by the small GTPase OsRac1 in rice, Taken together, these results showed that OsMT2b is an ROS scavenger and its expression is down-regulated by OsRac1, thus potentiating ROS, which function as signals in resistance response
- DTC1, OsMT2b, Defective Tapetum Cell Death 1 DTC1 Regulates ROS Levels by Binding to Metallothionein during Tapetum Degeneration., DTC1 protein interacts with OsMT2b, a ROS-scavenger
- OsMT2b, OsMTI2B~OsMT2c, Two metallothionein genes highly expressed in rice nodes are involved in distribution of Zn to the grain, Transcriptomic analysis of a rice node revealed that two metallothionein genes, OsMT2b and OsMT2c, were highly expressed in the node I
- OsMT2b, OsMTI2B~OsMT2c, Two metallothionein genes highly expressed in rice nodes are involved in distribution of Zn to the grain, Both OsMT2b and OsMT2c were mainly and constitutively expressed in the phloem region of enlarged and diffuse vascular bundles in the nodes and of the anther
- OsMT2b, OsMTI2B~OsMT2c, Two metallothionein genes highly expressed in rice nodes are involved in distribution of Zn to the grain, Knockout of either OsMT2b or OsMT2c increased zinc (Zn) accumulation in the nodes, but decreased Zn distribution to the panicle, resulting in decreased grain yield
- OsMT2b, OsMTI2B~OsMT2c, Two metallothionein genes highly expressed in rice nodes are involved in distribution of Zn to the grain, Both OsMT2b and OsMT2c showed binding ability with Zn, whereas only OsMT2b showed binding ability with Cu in yeast
- OsMT2b, OsMTI2B~OsMT2c, Two metallothionein genes highly expressed in rice nodes are involved in distribution of Zn to the grain, Our results suggest that both OsMT2b and OsMT2c play an important role mainly in the distribution of Zn to grain through chelation and subsequent transport of Zn in the phloem in rice
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