OsACBP2

• Symbol: OsACBP2
• MSU: LOC_Os06g02490
• RAPdb: Os06g0115300

• Subcellular localization of rice acyl-CoA-binding proteins ACBPs indicates that OsACBP6::GFP is targeted to the peroxisomes., 2014, New Phytol.
• Rice acyl-CoA-binding proteins OsACBP4 and OsACBP5 are differentially localized in the endoplasmic reticulum of transgenic Arabidopsis., 2014, Plant Signal Behav.
• The overexpression of rice ACYL-COA-BINDING PROTEIN2 increases grain size and bran oil content in transgenic rice., 2019, Plant J.
• Crystal structure of the rice acyl-CoA-binding protein OsACBP2 in complex with C18:3-CoA reveals a novel pattern of binding to acyl-CoA esters, 2020, FEBS Lett.

Genbank accession number

• Herein, rice OsACBP2 was demonstrated not only to play a role in seed development and germination, but also to influence grain size
• When OsACBP2 function was investigated using osacbp2 mutants and transgenic rice overexpressing OsACBP2 (OsACBP2-OE), osacbp2 was retarded in germination, while OsACBP2-OEs performed better than the wild-type and vector-transformed controls, in germination, seedling growth, grain size, and grain weight
• Deletion analysis of the OsACBP2 5’-flanking region revealed five copies of the seed cis-element, Skn-I-like motif (-1486/-1482, -956/-952, -939/-935, -826/-822, and -766/-762), and the removal of any adversely affected expression in seeds, thereby providing a molecular basis for OsACBP2 expression in seeds
• As dietary rice bran contains beneficial bioactive components, OsACBP2 appears to be a promising candidate for enriching seed nutritional value
• OsACBP2 mRNA accumulated in embryos and endosperm of germinating seeds in qRT-PCR analysis, while β-glucuronidase (GUS) assays on OsACBP2pro::GUS rice transformants showed GUS expression in embryos, as well as the scutellum and aleurone layer of germinating seeds

• OsACBP2, OsACBP6, Rice acyl-CoA-binding proteins OsACBP4 and OsACBP5 are differentially localized in the endoplasmic reticulum of transgenic Arabidopsis., In our recent paper in the New Phytologist, we reported that the six acyl-CoA-binding proteins (OsACBPs) in rice (Oryza sativa) are distributed across various subcellular compartments in transgenic Arabidopsis (Arabidopsis thaliana) such as the cytosol (OsACBP1, OsACBP2 and OsACBP3), the endoplasmic reticulum (ER) including the tubules (OsACBP4 and OsACBP5) and the cisternae (OsACBP4), and the peroxisomes (OsACBP6)