- Information
- Symbol: qCdT7,OsHMA3
- MSU: LOC_Os07g12900
- RAPdb: Os07g0232900
- Publication
- Functional analysis of the C-terminal region of the vacuolar cadmium-transporting rice OsHMA3, 2014, FEBS Lett.
- P1B-ATPases–an ancient family of transition metal pumps with diverse functions in plants, 2005, Trends Plant Sci.
- OsHMA3, a P1B-type of ATPase affects root-to-shoot cadmium translocation in rice by mediating efflux into vacuoles, 2011, New Phytol.
- Physiological, genetic, and molecular characterization of a high-Cd-accumulating rice cultivar, Jarjan, 2011, J Exp Bot.
- Gene limiting cadmium accumulation in rice, 2010, Proc Natl Acad Sci U S A.
- A single recessive gene controls cadmium translocation in the cadmium hyperaccumulating rice cultivar Cho-Ko-Koku, 2010, Theor Appl Genet.
- Overexpression of OsHMA3 enhances Cd tolerance and expression of Zn transporter genes in rice., 2014, J Exp Bot.
- A loss-of-function allele of OsHMA3 associated with high cadmium accumulation in shoots and grain of Japonica rice cultivars., 2016, Plant Cell Environ.
- Effective reduction of cadmium accumulation in rice grain by expressing OsHMA3 under the control of OsHMA2 promoter., 2018, J Exp Bot.
- Natural variation in the promoter of OsHMA3 contributes to differential grain cadmium accumulation between Indica and Japonica rice., 2019, J Integr Plant Biol.
- Map-based cloning of a new total loss-of-function allele of OsHMA3 causing high cadmium accumulation in rice grain., 2019, J Exp Bot.
- A tonoplast-localized OsHMA3 plays an important role in maintaining Zn homeostasis in rice., 2019, J Exp Bot.
- Comprehensive analysis of variation of cadmium accumulation in rice and detection of a new weak allele of OsHMA3., 2019, J Exp Bot.
- Calcium Plays a Double-Edged Role in Modulating Cadmium Uptake and Translocation in Rice, 2020, Int J Mol Sci.
- Comparative transcriptome profile analysis of rice varieties with different tolerance to zinc deficiency, 2020, Plant Biol (Stuttg).
- Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, 2020, PLoS One.
- Cadmium inhibits lateral root emergence in rice by disrupting OsPIN-mediated auxin distribution and the protective effect of OsHMA3, 2020, Plant Cell Physiol.
- Nanoparticle-based amelioration of drought stress and cadmium toxicity in rice via triggering the stress responsive genetic mechanisms and nutrient acquisition, 2020, Ecotoxicol Environ Saf.
- Genbank accession number
- Key message
- Furthermore, we isolated OsHMA3, a gene encoding a tonoplast-localized Cd transporter from Jarjan
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- In this study, using positional cloning and transgenic strategies, heavy metal ATPase 3 (OsHMA3) was identified as the gene that controls root-to-shoot Cd translocation rates
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- The allele of OsHMA3 that confers high root-to-shoot Cd translocation rates (OsHMA3mc) encodes a defective P(1B) -ATPase transporter
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- We propose that the root cell cytoplasm of Cd-overaccumulating rice plants has more Cd available for loading into the xylem as a result of the lack of OsHMA3-mediated transportation of Cd to the vacuoles
- OsHMA3, a P1B-type of ATPase affects root-to-shoot cadmium translocation in rice by mediating efflux into vacuoles
- Our results indicated that OsHMA3 from the low Cd-accumulating cultivar limits translocation of Cd from the roots to the above-ground tissues by selectively sequestrating Cd into the root vacuoles
- Rice OsHMA3 is a vacuolar cadmium (Cd) transporter belonging to the P1B-ATPase family and has a long (273aa) C-terminal region
- Functional analysis of the C-terminal region of the vacuolar cadmium-transporting rice OsHMA3
- The OsHMA3 protein was localized in all roots cells, but the sequence has a mutation leading to loss of function
- Therefore, failure to sequester Cd into the root vacuoles by OsHMA3 is probably responsible for high Cd accumulation in Jarjan
- Overexpression of OsHMA3 selectively reduces Cd accumulation in the grain
- Taken together, overexpression of OsHMA3 is an efficient way to reduce Cd accumulation in the grain and to enhance Cd tolerance in rice
- As a member of the heavy metal ATPase (HMA) family, OsHMA3 is a tonoplast-localized transporter for Cd in the roots of rice (Oryza sativa)
- Overexpression of OsHMA3 enhances Cd tolerance and expression of Zn transporter genes in rice.
- Further characterization in the present study revealed that overexpression of OsHMA3 also enhances the tolerance to toxic Cd
- A loss-of-function allele of OsHMA3 associated with high cadmium accumulation in shoots and grain of Japonica rice cultivars.
- Our study has identified a new loss-of-function allele of OsHMA3 in Japonica rice cultivars leading to high Cd accumulation in shoots and grain
- Furthermore, OsHMA3 protein was detected in the root pericycle cells and phloem region of both the diffuse vascular bundle and the enlarged vascular bundle of the nodes
- Effective reduction of cadmium accumulation in rice grain by expressing OsHMA3 under the control of OsHMA2 promoter.
- These results indicate that expression of OsHMA3 under the control of OsHMA2 promoter can effectively reduce Cd accumulation in rice grain through sequestering more Cd into the vacuoles of various tissues
- We found that the two GCC7 alleles, GCC7PA64s and GCC793-11 , had different promoter activity of OsHMA3, leading to different OsHMA3 expression and different shoot and grain Cd concentrations
- Natural variation in the promoter of OsHMA3 contributes to differential grain cadmium accumulation between Indica and Japonica rice.
- Our results thus reveal that the QTL GCC7 with sequence variation in the OsHMA3 promoter is an important determinant controlling differential grain Cd accumulation between Indica and Japonica rice
- Taken together, we have identified a new allele of OsHMA3 with a total loss of function, resulting in a greatly elevated Cd translocation to the shoots and grain of rice
- Map-based cloning of a new total loss-of-function allele of OsHMA3 causing high cadmium accumulation in rice grain.
- By comparing rice accessions with functional allele of OsHMA3 with accessions with loss of function allele of OsHMA3, we found that accessions with functional OsHMA3 showed higher tolerance to high Zn than those with non-functional OsHMA3 based on root elongation test
- When exposed to Zn-free solution, the Zn concentration in the root cell sap was rapidly decreased in the accessions with functional OsHMA3, but less changed in the accessions with non-functional OsHMA3
- A tonoplast-localized OsHMA3 plays an important role in maintaining Zn homeostasis in rice.
- Comprehensive analysis of variation of cadmium accumulation in rice and detection of a new weak allele of OsHMA3
- However, Ca application facilitated the upward translocation of Cd by both upregulating OsHMA2 to induce xylem loading of Cd and downregulating OsHMA3 to reduce vacuolar sequestration of Cd
- Meanwhile, application of sufficient doses of Fe and Cd in solution culture increased RE of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 in roots, whereas expression level of OsHMA3 was decreased
- Cadmium inhibits lateral root emergence in rice by disrupting OsPIN-mediated auxin distribution and the protective effect of OsHMA3
- Overexpression of functional OsHMA3 in rice greatly alleviated the inhibitory effect of Cd, but the protective effect of OsHMA3 was abolished by the auxin polar transport inhibitor 1-N-naphthylphthalamic acid
- The results suggest that Cd inhibits LR development in rice by disrupting OsPIN-mediated auxin distribution to LPR and OsHMA3 protects against Cd toxicity by sequestering Cd into the vacuoles
- The emergence of LRs in a rice cultivar with a null allele of OsHMA3 (Heavy Metal ATPase 3) was more sensitive to Cd than cultivars with functional alleles
- Furthermore, the expression of the Cd transporter genes, OsHMA2, OsHMA3 and OsLCT1 were curtailed in NPs-treated rice plants under normal and drought stress conditions
- Connection
- OsHMA2~OsHMA2v, qCdT7~OsHMA3, Effective reduction of cadmium accumulation in rice grain by expressing OsHMA3 under the control of OsHMA2 promoter., Effective reduction of cadmium accumulation in rice grain by expressing OsHMA3 under the control of OsHMA2 promoter.
- OsHMA2~OsHMA2v, qCdT7~OsHMA3, Effective reduction of cadmium accumulation in rice grain by expressing OsHMA3 under the control of OsHMA2 promoter., The aim of this study is to manipulate both expression and tissue localization of OsHMA3, a tonoplast-localized Cd transporter in the roots by expressing it under the control of OsHMA2 promoter, which shows high expression in different organs including roots, nodes and shoots
- OsHMA2~OsHMA2v, qCdT7~OsHMA3, Effective reduction of cadmium accumulation in rice grain by expressing OsHMA3 under the control of OsHMA2 promoter., These results indicate that expression of OsHMA3 under the control of OsHMA2 promoter can effectively reduce Cd accumulation in rice grain through sequestering more Cd into the vacuoles of various tissues
- OsZIP4, qCdT7~OsHMA3, A tonoplast-localized OsHMA3 plays an important role in maintaining Zn homeostasis in rice., Higher expression levels of OsZIP4, OsZIP5, OsZIP8 and OsZIP10 were found in the roots of accessions with functional OsHMA3 than those of accessions with non-functional OsHMA3 in response to Zn-deficiency
- OsZIP5, qCdT7~OsHMA3, A tonoplast-localized OsHMA3 plays an important role in maintaining Zn homeostasis in rice., Higher expression levels of OsZIP4, OsZIP5, OsZIP8 and OsZIP10 were found in the roots of accessions with functional OsHMA3 than those of accessions with non-functional OsHMA3 in response to Zn-deficiency
- OsZIP8, qCdT7~OsHMA3, A tonoplast-localized OsHMA3 plays an important role in maintaining Zn homeostasis in rice., Higher expression levels of OsZIP4, OsZIP5, OsZIP8 and OsZIP10 were found in the roots of accessions with functional OsHMA3 than those of accessions with non-functional OsHMA3 in response to Zn-deficiency
- qCdT7~OsHMA3, ZN, A tonoplast-localized OsHMA3 plays an important role in maintaining Zn homeostasis in rice., A tonoplast-localized OsHMA3 plays an important role in maintaining Zn homeostasis in rice.
- qCdT7~OsHMA3, ZN, A tonoplast-localized OsHMA3 plays an important role in maintaining Zn homeostasis in rice., Here, we found that OsHMA3, a tonoplast-localized transporter for Zn/Cd plays an important role in Zn homeostasis in rice
- qCdT7~OsHMA3, ZN, A tonoplast-localized OsHMA3 plays an important role in maintaining Zn homeostasis in rice., By comparing rice accessions with functional allele of OsHMA3 with accessions with loss of function allele of OsHMA3, we found that accessions with functional OsHMA3 showed higher tolerance to high Zn than those with non-functional OsHMA3 based on root elongation test
- qCdT7~OsHMA3, ZN, A tonoplast-localized OsHMA3 plays an important role in maintaining Zn homeostasis in rice., A 67Zn-labeling experiment showed that accessions with loss of function of OsHMA3 had lower Zn accumulation in the roots although similar Zn concentration was found in the shoots compared with accessions with functional OsHMA3
- qCdT7~OsHMA3, ZN, A tonoplast-localized OsHMA3 plays an important role in maintaining Zn homeostasis in rice., When exposed to Zn-free solution, the Zn concentration in the root cell sap was rapidly decreased in the accessions with functional OsHMA3, but less changed in the accessions with non-functional OsHMA3
- qCdT7~OsHMA3, ZN, A tonoplast-localized OsHMA3 plays an important role in maintaining Zn homeostasis in rice., A mobility experiment showed that more Zn in the roots was translocated to the shoots in the accessions with functional OsHMA3 compared with the accessions with non-functional OsHMA3
- qCdT7~OsHMA3, ZN, A tonoplast-localized OsHMA3 plays an important role in maintaining Zn homeostasis in rice., Taking together, our results indicate that OsHMA3 plays an important role in both Zn detoxification and storage by sequestering Zn into the vacuoles in rice roots, depending on Zn concentrations in the environment
- OsHMA2~OsHMA2v, qCdT7~OsHMA3, Calcium Plays a Double-Edged Role in Modulating Cadmium Uptake and Translocation in Rice, However, Ca application facilitated the upward translocation of Cd by both upregulating OsHMA2 to induce xylem loading of Cd and downregulating OsHMA3 to reduce vacuolar sequestration of Cd
- OsNRAMP5, qCdT7~OsHMA3, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Fe, Mn, and Cd transporters such as CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, OsHMA3, and OsNAAT1 regulate uptake of Cd in rice
- OsNRAMP5, qCdT7~OsHMA3, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Meanwhile, application of sufficient doses of Fe and Cd in solution culture increased RE of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 in roots, whereas expression level of OsHMA3 was decreased
- OsNRAMP5, qCdT7~OsHMA3, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, This may be concluded that the Cd increases expression of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 but decreases OsHMA3 expression in rice roots, which resulted in increased Cd uptake in hydroponically grown rice
- OsNRAMP1, qCdT7~OsHMA3, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Fe, Mn, and Cd transporters such as CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, OsHMA3, and OsNAAT1 regulate uptake of Cd in rice
- OsNRAMP1, qCdT7~OsHMA3, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Meanwhile, application of sufficient doses of Fe and Cd in solution culture increased RE of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 in roots, whereas expression level of OsHMA3 was decreased
- OsNRAMP1, qCdT7~OsHMA3, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, This may be concluded that the Cd increases expression of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 but decreases OsHMA3 expression in rice roots, which resulted in increased Cd uptake in hydroponically grown rice
- OsDEF7~CAL1~OsAFP1, qCdT7~OsHMA3, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Fe, Mn, and Cd transporters such as CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, OsHMA3, and OsNAAT1 regulate uptake of Cd in rice
- OsDEF7~CAL1~OsAFP1, qCdT7~OsHMA3, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Meanwhile, application of sufficient doses of Fe and Cd in solution culture increased RE of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 in roots, whereas expression level of OsHMA3 was decreased
- OsDEF7~CAL1~OsAFP1, qCdT7~OsHMA3, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, This may be concluded that the Cd increases expression of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 but decreases OsHMA3 expression in rice roots, which resulted in increased Cd uptake in hydroponically grown rice
- OsIRT1, qCdT7~OsHMA3, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Fe, Mn, and Cd transporters such as CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, OsHMA3, and OsNAAT1 regulate uptake of Cd in rice
- OsIRT1, qCdT7~OsHMA3, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Meanwhile, application of sufficient doses of Fe and Cd in solution culture increased RE of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 in roots, whereas expression level of OsHMA3 was decreased
- OsIRT1, qCdT7~OsHMA3, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, This may be concluded that the Cd increases expression of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 but decreases OsHMA3 expression in rice roots, which resulted in increased Cd uptake in hydroponically grown rice
- OsNAAT1, qCdT7~OsHMA3, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Fe, Mn, and Cd transporters such as CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, OsHMA3, and OsNAAT1 regulate uptake of Cd in rice
- OsNAAT1, qCdT7~OsHMA3, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, Meanwhile, application of sufficient doses of Fe and Cd in solution culture increased RE of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 in roots, whereas expression level of OsHMA3 was decreased
- OsNAAT1, qCdT7~OsHMA3, Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment, This may be concluded that the Cd increases expression of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 but decreases OsHMA3 expression in rice roots, which resulted in increased Cd uptake in hydroponically grown rice
- OsHMA2~OsHMA2v, qCdT7~OsHMA3, Nanoparticle-based amelioration of drought stress and cadmium toxicity in rice via triggering the stress responsive genetic mechanisms and nutrient acquisition, Furthermore, the expression of the Cd transporter genes, OsHMA2, OsHMA3 and OsLCT1 were curtailed in NPs-treated rice plants under normal and drought stress conditions
- OsLCT1, qCdT7~OsHMA3, Nanoparticle-based amelioration of drought stress and cadmium toxicity in rice via triggering the stress responsive genetic mechanisms and nutrient acquisition, Furthermore, the expression of the Cd transporter genes, OsHMA2, OsHMA3 and OsLCT1 were curtailed in NPs-treated rice plants under normal and drought stress conditions
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