- Information
- Symbol: OsSPL14,IPA1,WFP
- MSU: LOC_Os08g39890
- RAPdb: Os08g0509600
- Publication
- Regulation of OsSPL14 by OsmiR156 defines ideal plant architecture in rice, 2010, Nat Genet.
- OsSPL14 promotes panicle branching and higher grain productivity in rice, 2010, Nat Genet.
- Shaping a better rice plant, 2010, Nat Genet.
- Genome-wide binding analysis of the transcription activator ideal plant architecture1 reveals a complex network regulating rice plant architecture, 2013, Plant Cell.
- Enhanced expression of OsSPL14 gene and its association with yield components in rice Oryza sativa under low nitrogen conditions., 2015, Gene.
- A natural tandem array alleviates epigenetic repression of IPA1 and leads to superior yielding rice., 2017, Nat Commun.
- IPA1 functions as a downstream transcription factor repressed by D53 in strigolactone signaling in rice., 2017, Cell Res.
- Introgression of a functional epigenetic OsSPL14WFPallele into elite indica rice genomes greatly improved panicle traits and grain yield., 2018, Sci Rep.
- A single transcription factor promotes both yield and immunity in rice., 2018, Science.
- Inducible overexpression of Ideal Plant Architecture1 improves both yield and disease resistance in rice., 2019, Nat Plants.
- MiR529a controls plant height, tiller number, panicle architecture and grain size by regulating SPL target genes in rice Oryza sativa L., 2021, Plant Sci.
- IPA1 Negatively Regulates Early Rice Seedling Development by Interfering with Starch Metabolism via the GA and WRKY Pathways, 2021, Int J Mol Sci.
- ipa1 improves rice drought tolerance at seedling stage mainly through activating abscisic acid pathway., 2021, Plant Cell Rep.
- Osa-miR535 targets SQUAMOSA promoter binding protein-like 4 to regulate blast disease resistance in rice., 2022, Plant J.
- Targeting a gene regulatory element enhances rice grain yield by decoupling panicle number and size., 2022, Nat Biotechnol.
- IPA1 improves drought tolerance by activating SNAC1 in rice., 2023, BMC Plant Biol.
- Genbank accession number
- Key message
- Two studies describe how regulatory variation at the rice gene OsSPL14 can lead to altered plant morphology and improve grain yield
- We demonstrate that a point mutation in OsSPL14 perturbs OsmiR156-directed regulation of OsSPL14, generating an ‘ideal’ rice plant with a reduced tiller number, increased lodging resistance and enhanced grain yield
- Moreover, our results demonstrated that IPA1 could directly bind to the promoter of rice teosinte branched1, a negative regulator of tiller bud outgrowth, to suppress rice tillering, and directly and positively regulate dense and erect panicle1, an important gene regulating panicle architecture, to influence plant height and panicle length
- Here, we report the cloning and characterization of a semidominant quantitative trait locus, IPA1 (Ideal Plant Architecture 1), which profoundly changes rice plant architecture and substantially enhances rice grain yield
- We also demonstrate the feasibility of using the OsSLP14(WFP) allele to increase rice crop yield
- Introduction of the high-yielding OsSPL14(WFP) allele into the standard rice variety Nipponbare resulted in increased rice production
- Ideal plant architecture1 (IPA1) is critical in regulating rice (Oryza sativa) plant architecture and substantially enhances grain yield
- The elucidation of target genes of IPA1 genome-wide will contribute to understanding the molecular mechanisms underlying plant architecture and to facilitating the breeding of elite varieties with ideal plant architecture
- Higher expression of OsSPL14 in the reproductive stage promotes panicle branching and higher grain yield in rice
- OsSPL14 promotes panicle branching and higher grain productivity in rice
- OsSPL14 controls shoot branching in the vegetative stage and is affected by microRNA excision
- Our study suggests that OsSPL14 may help improve rice grain yield by facilitating the breeding of new elite rice varieties
- Here we show that the quantitative trait locus WFP (WEALTHY FARMER’S PANICLE) encodes OsSPL14 (SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 14, also known as IPA1)
- To elucidate its molecular basis, we first confirmed IPA1 as a functional transcription activator and then identified 1067 and 2185 genes associated with IPA1 binding sites in shoot apices and young panicles, respectively, through chromatin immunoprecipitation sequencing assays
- Regulation of OsSPL14 by OsmiR156 defines ideal plant architecture in rice
- Expression analysis of OsSPL14 (LOC_Os08g39890) gene reported to be associated with increased panicle branching and higher grain yield through real time PCR in leaf and three stages of panicle has shown differential temporal expression and its association with yield and yield related components across the genotypes
- Since OsSPL14 is a functional transcription activator, its association of expression in leaf and three panicle stages with yield components as observed in the present study suggests the role of nitrogen metabolism related genes in plant growth and development and its conversion into yield components in rice
- The expression of OsSPL14 at panicle stage 3, has shown correlation (P<0
- Enhanced expression of OsSPL14 gene and its association with yield components in rice (Oryza sativa) under low nitrogen conditions.
- IPA1 functions as a downstream transcription factor repressed by D53 in strigolactone signaling in rice.
- These findings reveal that IPA1 is likely one of the long-speculated transcription factors that act with D53 to mediate the SL-regulated tiller development in rice
- IPA1 returns to a nonphosphorylated state within 48 hours after infection, resuming support of the growth needed for high yield
- Thus, IPA1 promotes both yield and disease resistance by sustaining a balance between growth and immunity
- Phosphorylated IPA1 binds to the promoter of the pathogen defense gene WRKY45 and activates its expression, leading to enhanced disease resistance
- Mechanistically, phosphorylation of IPA1 at amino acid Ser163 within its DNA binding domain occurs in response to infection by the fungus Magnaporthe oryzae and alters the DNA binding specificity of IPA1
- Here we report that higher IPA1 levels enhance immunity
- We discovered that gibberellin signalling might be partially responsible for the disease resistance and developmental defects in IPA1 overexpressors
- We then generated transgenic rice plants expressing IPA1 with the pathogen-inducible promoter of OsHEN1; these plants had both enhanced disease resistance and enhanced yield-related traits
- Using transient dual-luciferase and yeast one-hybrid assays, IPA1 was found to directly activate the expression of OsWRKY51 and OsWRKY71, which would interfere with the binding affinity of GA-induced transcription factor OsGAMYB to inhibit the expression of
-amylase genes - To investigate the role of IPA1 in early seedling development, we developed a pair of IPA1/ipal-NILs and found that seed germination and early seedling growth were retarded in the ipa1-NIL
- In summary, our results suggest that IPA1 negatively regulates seed germination and early seedling growth by interfering with starch metabolism via the GA and WRKY pathways
- IPA1 Negatively Regulates Early Rice Seedling Development by Interfering with Starch Metabolism via the GA and WRKY Pathways
- The ipa1 plants had a better-developed root system and smaller leaf stomatal aperture
- IPA1 (IDEAL PLANT ARCHITECTURE 1)/OsSPL14 encodes a transcription factor and has been reported to function in both rice ideal plant architecture and biotic resistance
- In addition, based on yeast one-hybrid assay and dual-luciferase assay, IPA1 was found to directly activate the promoter activity of OsHOX12, a transcription factor promoting ABA biosynthesis, and OsNAC52, a positive regulator of the ABA pathway
- In both the control and PEG-treated conditions, ABA content in the ipa1 seedlings was significantly higher than that in the IPA1 seedlings
- Expression of the ABA biosynthesis genes was detected to be up-regulated, whereas the expression of ABA catabolism genes was down-regulated in the ipa1 seedlings
- Combined with the previous studies, our results suggested that ipa1 could improve rice seedling drought tolerance mainly through activating the ABA pathway and that regulation of the ipa1-mediated ABA pathway will be an important strategy for improving drought resistance of rice
- ipa1 improves rice drought tolerance at seedling stage mainly through activating abscisic acid pathway.
- Here, with a pair of IPA1 and ipa1-NILs (Near Iso-genic Lines), we found that ipa1 could significantly improve rice drought tolerance at seedling stage
- KEY MESSAGE: ipa1 enhances rice drought tolerance mainly through activating the ABA pathway
- Mechanistic studies revealed that the deleted fragment is a target site for the transcription factor An-1 to repress IPA1 expression in panicles and roots
- In this study, we identified a 54-base pair cis-regulatory region in IPA1 via a tiling-deletion-based CRISPR-Cas9 screen that, when deleted, resolves the tradeoff between grains per panicle and tiller number, leading to substantially enhanced grain yield per plant
- Crop genetic improvement requires balancing complex tradeoffs caused by gene pleiotropy and linkage drags, as exemplified by IPA1 (Ideal Plant Architecture 1), a typical pleiotropic gene in rice that increases grains per panicle but reduces tillers
- IPA1 improves drought tolerance by activating SNAC1 in rice.
- Together, these findings indicated that the IPA1 played important roles in drought tolerance by regulating SNAC1, thus activating the antioxidant system in rice
- Further investigation indicated that the IPA1 plays a positive role in drought-stress tolerance by inducing reactive oxygen species scavenging in rice
- The IPA1 is constitutively expressed and regulated by H(2)O(2), abscisic acid, NaCl and polyethylene glycol 6000 treatments in rice
- Connection
- Osmtd1, OsSPL14~IPA1~WFP, A T-DNA insertion mutant Osmtd1 aas altered in architecture by upregulating MicroRNA156f in rice., We showed that the expression of OsSPL3, OsSPL12 and OsSPL14 was significantly down-regulated in Osmtd1 or OsmiR156f overexpressed lines, indicating that OsSPL3, OsSPL12 and OsSPL14 were possibly direct target genes of OsmiR156f
- IPI1, OsSPL14~IPA1~WFP, Tissue-specific Ubiquitination by IPA1 INTERACTING PROTEIN 1 Modulates IPA1 Protein Levels to Regulate Plant Architecture in Rice., Here, we report the identification and characterization of IPA1 INTERACTING PROTEIN 1 (IPI1), a RING-finger E3 ligase that can interact with IPA1 in the nucleus
- IPI1, OsSPL14~IPA1~WFP, Tissue-specific Ubiquitination by IPA1 INTERACTING PROTEIN 1 Modulates IPA1 Protein Levels to Regulate Plant Architecture in Rice., IPI1 promotes the degradation of IPA1 in panicles while it stabilizes IPA1 in shoot apexes
- IPI1, OsSPL14~IPA1~WFP, Tissue-specific Ubiquitination by IPA1 INTERACTING PROTEIN 1 Modulates IPA1 Protein Levels to Regulate Plant Architecture in Rice., These results demonstrate that IPI1 affects plant architecture through precisely tuning IPA1 protein levels in different tissues in rice, and provide new insight into the tissue-specific regulation of plant architecture and important genetic resources for molecular breeding applications
- OsSPL14~IPA1~WFP, WTG1~OsOTUB1~GWC1, Non-canonical regulation of SPL transcription factors by a human OTUB1-like deubiquitinase defines a new plant type rice associated with higher grain yield., OsOTUB1 interacts with the E2 ubiquitin-conjugating protein OsUBC13 and the squamosa promoter-binding protein-like transcription factor OsSPL14
- OsSPL14~IPA1~WFP, WTG1~OsOTUB1~GWC1, Non-canonical regulation of SPL transcription factors by a human OTUB1-like deubiquitinase defines a new plant type rice associated with higher grain yield., OsOTUB1 and OsSPL14 share common target genes, and their physical interaction limits K63-linked ubiquitination (K63Ub) of OsSPL14, which in turn promotes K48Ub-dependent proteasomal degradation of OsSPL14
- OsSPL14~IPA1~WFP, WTG1~OsOTUB1~GWC1, Non-canonical regulation of SPL transcription factors by a human OTUB1-like deubiquitinase defines a new plant type rice associated with higher grain yield., Conversely, loss-of-function of OsOTUB1 is correlated with the accumulation of high levels of OsSPL14, resulting in the NPT architecture
- D53, OsSPL14~IPA1~WFP, IPA1 functions as a downstream transcription factor repressed by D53 in strigolactone signaling in rice., IPA1 functions as a downstream transcription factor repressed by D53 in strigolactone signaling in rice.
- D53, OsSPL14~IPA1~WFP, IPA1 functions as a downstream transcription factor repressed by D53 in strigolactone signaling in rice., Here we report that Ideal Plant Architecture 1 (IPA1), a key regulator of the plant architecture in rice, functions as a direct downstream component of D53 in regulating tiller number and SL-induced gene expression
- D53, OsSPL14~IPA1~WFP, IPA1 functions as a downstream transcription factor repressed by D53 in strigolactone signaling in rice., We showed that D53 interacts with IPA1 in vivo and in vitro and suppresses the transcriptional activation activity of IPA1
- D53, OsSPL14~IPA1~WFP, IPA1 functions as a downstream transcription factor repressed by D53 in strigolactone signaling in rice., We further showed that IPA1 could directly bind to the D53 promoter and plays a critical role in the feedback regulation of SL-induced D53 expression
- D53, OsSPL14~IPA1~WFP, IPA1 functions as a downstream transcription factor repressed by D53 in strigolactone signaling in rice., These findings reveal that IPA1 is likely one of the long-speculated transcription factors that act with D53 to mediate the SL-regulated tiller development in rice
- OsSPL14~IPA1~WFP, OsWRKY45~WRKY45, A single transcription factor promotes both yield and immunity in rice., Phosphorylated IPA1 binds to the promoter of the pathogen defense gene WRKY45 and activates its expression, leading to enhanced disease resistance
- OsSPL14~IPA1~WFP, OsSPL7, Inducible overexpression of Ideal Plant Architecture1 improves both yield and disease resistance in rice., In this study, we found that downregulation of microRNA-156 (miR-156) and overexpression of Ideal Plant Architecture1 (IPA1) and OsSPL7, two target genes of miR-156, enhanced disease resistance against bacterial blight caused by Xanthomonas oryzae pv
- OsSHI1, OsSPL14~IPA1~WFP, OsSHI1 Regulates Plant Architecture Through Modulating the Transcriptional Activity of IPA1 in Rice., OsSHI1 Regulates Plant Architecture Through Modulating the Transcriptional Activity of IPA1 in Rice.
- OsSHI1, OsSPL14~IPA1~WFP, OsSHI1 Regulates Plant Architecture Through Modulating the Transcriptional Activity of IPA1 in Rice., We show that OsSHI1 physically interacts with IPA1 both in vitro and in vivo
- OsSHI1, OsSPL14~IPA1~WFP, OsSHI1 Regulates Plant Architecture Through Modulating the Transcriptional Activity of IPA1 in Rice., Further, OsSHI1 represses the transcriptional activation activity of IPA1 by affecting its DNA binding activity towards the promoters of both OsTB1 and OsDEP1, resulting in increased tiller number and diminished panicle size
- OsSHI1, OsSPL14~IPA1~WFP, OsSHI1 Regulates Plant Architecture Through Modulating the Transcriptional Activity of IPA1 in Rice., Taken together, our results demonstrate that OsSHI1 regulates plant architecture through modulating the transcriptional activity of IPA1 and provide novel insight into the establishment of plant architecture in rice
- miR156, OsSPL14~IPA1~WFP, The grain yield modulator miR156 regulates seed dormancy through the gibberellin pathway in rice., Mechanistically, mir156 mutations enhance seed dormancy by suppressing the gibberellin (GA) pathway through de-represssion of the miR156 target gene Ideal Plant Architecture 1 (IPA1), which directly regulates multiple genes in the GA pathway
- OsSPL12, OsSPL14~IPA1~WFP, Seed-Specific Overexpression of SPL12 and IPA1 Improves Seed Dormancy and Grain Size in Rice, Seed-Specific Overexpression of SPL12 and IPA1 Improves Seed Dormancy and Grain Size in Rice
- OsSPL14~IPA1~WFP, qGW8~OsSPL16~GW8, MiR529a controls plant height, tiller number, panicle architecture and grain size by regulating SPL target genes in rice Oryza sativa L., And panicle architecture and grain size were controlled by miR529a through altering the expression of all five target genes OsSPL2, OsSPL7, OsSPL14, OsSPL16, OsSPL17 and OsSPL18
- OsSPL14~IPA1~WFP, OsSPL18, MiR529a controls plant height, tiller number, panicle architecture and grain size by regulating SPL target genes in rice Oryza sativa L., And panicle architecture and grain size were controlled by miR529a through altering the expression of all five target genes OsSPL2, OsSPL7, OsSPL14, OsSPL16, OsSPL17 and OsSPL18
- OsSPL14~IPA1~WFP, OsSPL7, MiR529a controls plant height, tiller number, panicle architecture and grain size by regulating SPL target genes in rice Oryza sativa L., And panicle architecture and grain size were controlled by miR529a through altering the expression of all five target genes OsSPL2, OsSPL7, OsSPL14, OsSPL16, OsSPL17 and OsSPL18
- OsMYBGA~OsGAMYB, OsSPL14~IPA1~WFP, IPA1 Negatively Regulates Early Rice Seedling Development by Interfering with Starch Metabolism via the GA and WRKY Pathways, Using transient dual-luciferase and yeast one-hybrid assays, IPA1 was found to directly activate the expression of OsWRKY51 and OsWRKY71, which would interfere with the binding affinity of GA-induced transcription factor OsGAMYB to inhibit the expression of α-amylase genes
- OsSPL14~IPA1~WFP, OsWRKY51, IPA1 Negatively Regulates Early Rice Seedling Development by Interfering with Starch Metabolism via the GA and WRKY Pathways, Using transient dual-luciferase and yeast one-hybrid assays, IPA1 was found to directly activate the expression of OsWRKY51 and OsWRKY71, which would interfere with the binding affinity of GA-induced transcription factor OsGAMYB to inhibit the expression of α-amylase genes
- OsSPL14~IPA1~WFP, OsWRKY71, IPA1 Negatively Regulates Early Rice Seedling Development by Interfering with Starch Metabolism via the GA and WRKY Pathways, Using transient dual-luciferase and yeast one-hybrid assays, IPA1 was found to directly activate the expression of OsWRKY51 and OsWRKY71, which would interfere with the binding affinity of GA-induced transcription factor OsGAMYB to inhibit the expression of α-amylase genes
- HOX12~Oshox12, OsSPL14~IPA1~WFP, ipa1 improves rice drought tolerance at seedling stage mainly through activating abscisic acid pathway., In addition, based on yeast one-hybrid assay and dual-luciferase assay, IPA1 was found to directly activate the promoter activity of OsHOX12, a transcription factor promoting ABA biosynthesis, and OsNAC52, a positive regulator of the ABA pathway
- HOX12~Oshox12, OsSPL14~IPA1~WFP, ipa1 improves rice drought tolerance at seedling stage mainly through activating abscisic acid pathway., The expression of OsHOX12 and OsNAC52 was significantly up-regulated in the ipa1 plants
- OsNAC52, OsSPL14~IPA1~WFP, ipa1 improves rice drought tolerance at seedling stage mainly through activating abscisic acid pathway., In addition, based on yeast one-hybrid assay and dual-luciferase assay, IPA1 was found to directly activate the promoter activity of OsHOX12, a transcription factor promoting ABA biosynthesis, and OsNAC52, a positive regulator of the ABA pathway
- OsNAC52, OsSPL14~IPA1~WFP, ipa1 improves rice drought tolerance at seedling stage mainly through activating abscisic acid pathway., The expression of OsHOX12 and OsNAC52 was significantly up-regulated in the ipa1 plants
- An-1, OsSPL14~IPA1~WFP, Targeting a gene regulatory element enhances rice grain yield by decoupling panicle number and size., Mechanistic studies revealed that the deleted fragment is a target site for the transcription factor An-1 to repress IPA1 expression in panicles and roots
- OsSPL14~IPA1~WFP, PILS6b, OsSPL14 acts upstream of OsPIN1b and PILS6b to modulate axillary bud outgrowth by fine-tuning auxin transport in rice., OsSPL14 acts upstream of OsPIN1b and PILS6b to modulate axillary bud outgrowth by fine-tuning auxin transport in rice.
- OsSPL14~IPA1~WFP, PILS6b, OsSPL14 acts upstream of OsPIN1b and PILS6b to modulate axillary bud outgrowth by fine-tuning auxin transport in rice., Further analysis revealed that OsSPL14 directly binds to the promoter of PIN-FORMED 1b (OsPIN1b) and PIN-LIKE6b (PILS6b) to positively regulate their expression
- OsSPL14~IPA1~WFP, PILS6b, OsSPL14 acts upstream of OsPIN1b and PILS6b to modulate axillary bud outgrowth by fine-tuning auxin transport in rice., Taken together, our findings suggested that OsSPL14 can control axillary bud outgrowth and tiller number by activating the expression of OsPIN1b and PILS6b to fine-tune auxin transport in rice
- OsSPL14~IPA1~WFP, SAPK6~OSRK1~OsSAPK6, Chilling-induced phosphorylation of IPA1 by OsSAPK6 activates chilling tolerance responses in rice., Chilling-induced phosphorylation of IPA1 by OsSAPK6 activates chilling tolerance responses in rice.
- OsSPL14~IPA1~WFP, SAPK6~OSRK1~OsSAPK6, Chilling-induced phosphorylation of IPA1 by OsSAPK6 activates chilling tolerance responses in rice., Under chilling stress, OsSAPK6 could phosphorylate IPA1 and increase its stability
- OsSPL14~IPA1~WFP, SAPK6~OSRK1~OsSAPK6, Chilling-induced phosphorylation of IPA1 by OsSAPK6 activates chilling tolerance responses in rice., Genetic evidence showed that OsSAPK6, IPA1 and OsCBF3 were all positive regulators of rice chilling tolerance
- OsSPL14~IPA1~WFP, SAPK6~OSRK1~OsSAPK6, Chilling-induced phosphorylation of IPA1 by OsSAPK6 activates chilling tolerance responses in rice., The function of OsSAPK6 in chilling tolerance depended on IPA1, and overexpression of OsCBF3 could rescue the chilling-sensitive phenotype of ipa1 loss-of-function mutant
- OsMPK6~OsMPK4, OsSPL14~IPA1~WFP, OsMPK4 promotes phosphorylation and degradation of IPA1 in response to salt stress to confer salt tolerance in rice., OsMPK4 promotes phosphorylation and degradation of IPA1 in response to salt stress to confer salt tolerance in rice.
- OsMPK6~OsMPK4, OsSPL14~IPA1~WFP, OsMPK4 promotes phosphorylation and degradation of IPA1 in response to salt stress to confer salt tolerance in rice., Under salt stress, OsMPK4 could interact with IPA1 and phosphorylate IPA1 at Thr180, leading to degradation of IPA1
- OsMPK6~OsMPK4, OsSPL14~IPA1~WFP, OsMPK4 promotes phosphorylation and degradation of IPA1 in response to salt stress to confer salt tolerance in rice., Genetic evidence shows that IPA1 is a negative regulator of salt tolerance in rice, whereas OsMPK4 promotes salt response in an IPA1-dependent manner
- LM7~OsHSP40~NAL11, OsSPL14~IPA1~WFP, The DnaJ domain-containing heat-shock protein NAL11 determines plant architecture by mediating gibberellin homeostasis in rice Oryza sativa., Biochemical analyses show that IPA1 directly binds to elements in the missing fragment of the NAL11(-923del-1552) promoter and negatively regulates NAL11 expression
- LM7~OsHSP40~NAL11, OsSPL14~IPA1~WFP, The DnaJ domain-containing heat-shock protein NAL11 determines plant architecture by mediating gibberellin homeostasis in rice Oryza sativa., Genetic analyses support the hypothesis that NAL11 acts downstream of IPA1 to regulate IPA by modulating GA homeostasis, and NAL11 may be an essential complement for IPA1
- OsNAC19~SNAC1~OsNAC9, OsSPL14~IPA1~WFP, IPA1 improves drought tolerance by activating SNAC1 in rice., IPA1 improves drought tolerance by activating SNAC1 in rice.
- OsNAC19~SNAC1~OsNAC9, OsSPL14~IPA1~WFP, IPA1 improves drought tolerance by activating SNAC1 in rice., Yeast one-hybrid, dual-luciferase and electrophoretic mobility shift assays indicated that the IPA1 directly activates the promoter of SNAC1
- OsNAC19~SNAC1~OsNAC9, OsSPL14~IPA1~WFP, IPA1 improves drought tolerance by activating SNAC1 in rice., Expression of SNAC1 is significantly down-regulated in IPA1 knockout plants
- OsNAC19~SNAC1~OsNAC9, OsSPL14~IPA1~WFP, IPA1 improves drought tolerance by activating SNAC1 in rice., Together, these findings indicated that the IPA1 played important roles in drought tolerance by regulating SNAC1, thus activating the antioxidant system in rice
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