| Categories genes  | Tags drought  pollen  ABA  anther  drought tolerance  fertility  anther development  tapetal  vegetative  yield  tapetum  abiotic stress  reproductive  drought resistance 
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  • Key message
    • To explore the potential gene function for improving rice reproductive development under drought, a drought induced gene, Oryza sativa Drought-Induced LTP (OsDIL) encoding a lipid transfer protein, was identified from our microarray data and selected for further study
    • OsDIL was primarily expressed in the anther and mainly responsive to abiotic stresses, including drought, cold, NaCl, and stress-related plant hormone abscisic acid (ABA)
    • Compared with wild type, the OsDIL-overexpressing transgenic rice plants were more tolerant to drought stress during vegetative development and showed less severe tapetal defects and fewer defective anther sacs when treated with drought at the reproductive stage
    • The expression levels of the drought-responsive genes RD22, SODA1, bZIP46 and POD, as well as the ABA synthetic gene ZEP1 were up-regulated in the OsDIL-overexpression lines but the ABA degradation gene ABAOX3 was down-regulated
    • Moreover, overexpression of OsDIL lessened the down-regulation by drought of anther developmental genes (OsC4, CYP704B2 and OsCP1), providing a mechanism supporting pollen fertility under drought
    • Overexpression of OsDIL significantly enhanced drought resistance in transgenic rice during reproductive development, while showing no phenotypic changes or yield penalty under normal conditions
    • The rice OsDIL gene plays a role in drought tolerance at vegetative and reproductive stages
    • Here, we isolated a novel rice anther-specific plant lipid transfer protein (OsLTP6) gene through high through-put expressional profiling
    • Histochemical and fluorometric GUS assay showed that GUS was specifically expressed in the anthers and pollens in OsLTP6 promoter::uidA transgenic plants
    • Transverse section of the rice anther further indicated that the OsLTP6 promoter directed the reporter gene specifically expressed in anther tapetum
    • The results indicated that the OsLTP6 promoter achieved anther-specific expression through a combination of positive and negative regulatory elements
    • The rice OsLTP6 gene promoter directs anther-specific expression by a combination of positive and negative regulatory elements
    • Therefore, OsDIL is an excellent candidate gene for genetic improvement of crop yield in adaption to unfavorable environments
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