OsPRP1

• Symbol: OsPRP1
• MSU: LOC_Os10g06000
• RAPdb: Os10g0150800

• Knockout of OsPRP1, a gene encoding proline-rich protein, confers enhanced cold sensitivity in rice Oryza sativa L. at the seedling stage., 2019, 3 Biotech.

Genbank accession number

• The OsPRP1 mutant plants displayed more sensitivity to cold stress and showed low survival rate with decreased root biomass than wild-type (WT) and homozygous mutant line with large fragment deletion was more sensitive to low temperature
• These results suggested that OsPRP1 enhances cold tolerance by modulating antioxidants and maintaining cross talk through signaling pathways
• Therefore, OsPRP1 gene could be exploited for improving cold tolerance in rice and CRISPR/Cas9 technology is helpful to study the function of a gene by analyzing the phenotypes of knockout mutants generated
• Mutant plants were generated by CRISPR/Cas9 technology to investigate the role of OsPRP1 in cold stress and 26 mutant plants were obtained in T0 generation with the mutation rate of 85% including 15% bi-allelic, 53