- Information
- Symbol: OsRad51,OsRad51A1,COLD11
- MSU: LOC_Os11g40150
- RAPdb: Os11g0615800
- Publication
- Homologous recombination properties of OsRad51, a recombinase from rice, 2008, Plant Mol Biol.
- Rice OsBRCA2 Is Required for DNA Double-Strand Break Repair in Meiotic Cells, 2020, Front Plant Sci ..
- OsRAD51 Plays a Vital Role in Promoting Homologous Recombination in Rice Meiosis., 2022, Int J Mol Sci.
- Natural variation of codon repeats in COLD11 endows rice with chilling resilience., 2023, Sci Adv.
- Genbank accession number
- Key message
- cDNA corresponding to OsRad51 protein was isolated from cDNA library of rice flowers (Oryza sativa, Indica cultivar group) and cloned in to pET28a expression vector
- These results indicate that OsBRCA2 is essential for facilitating the loading of OsRAD51 and OsDMC1 onto resected ends of programmed double-strand breaks (DSB) during meiosis to promote single-end invasions of homologous chromosomes and accurate recombination
- In the absence of OsBRCA2, localization to the meiotic chromosome axes of the strand-invasion proteins OsRAD51 and OsDMC1 is severely reduced and in vitro OsBRCA2 directly interacts with OsRAD51 and OsDMC1
- Osrad51 (Osrad51a1 Osrad51a2) mutant plants show normal vegetative growth but exhibit complete sterility, indicating that OsRAD51A1 and OsRAD51A2 are functionally redundant in rice fertility
- Moreover, univalents and multivalent associations were observed at metaphase I, chromosome fragments presented at anaphase I, and crossover formation is basically suppressed in Osrad51 pollen mother cells (PMCs)
- In the homologous recombination pathway, the detailed mechanisms of how OsRAD51 and OsDMC1 work in rice meiosis remain to be explored
- In addition, both OsRAD51 and OsDMC1 can interact with OsFIGL1 and OsBRCA2, two important components in rice meiosis
- OsRAD51 Plays a Vital Role in Promoting Homologous Recombination in Rice Meiosis.
- Genome evolution analysis of representative rice germplasms suggested that numbers of GCG sequence repeats in the first exon of COLD11 were subjected to strong domestication selection during the northern expansion of rice planting
- Rice loss-of-function mutations of COLD11 caused reduced chilling tolerance
- Natural variation of codon repeats in COLD11 endows rice with chilling resilience.
- Connection
- OsNAPL6, OsRad51~OsRad51A1~COLD11, A NAP-family histone chaperone acts in abiotic stress response and adaptation., For instance, we show that OsNAPL6 is recruited to OsRad51 promoter activating its expression, leading to a more efficient DNA repair and abrogation of programmed cell death under salinity and genotoxic stress conditions
- OsRad51~OsRad51A1~COLD11, OsRAD51D, OsRAD51D promotes homologous pairing and recombination by preventing non-homologous interactions in rice meiosis., The failed localization of OsRAD51 and OsDMC1 in Osrad51d, together with the genetic analysis of Osrad51d Osdmc1a Osdmc1b, indicates that OsRAD51D acts at a very early stage of HR
- OsRad51~OsRad51A1~COLD11, OsRAD51D, OsRAD51D promotes homologous pairing and recombination by preventing nonhomologous interactions in rice meiosis , The failed localization of OsRAD51 and OsDMC1 in Osrad51d, together with the genetic analysis of Osrad51d Osdmc1a Osdmc1b, indicates that OsRAD51D acts at a very early stage of homologous recombination
- OsDMC1~DMC1B~OsDMC1B, OsRad51~OsRad51A1~COLD11, Rice OsBRCA2 Is Required for DNA Double-Strand Break Repair in Meiotic Cells, In the absence of OsBRCA2, localization to the meiotic chromosome axes of the strand-invasion proteins OsRAD51 and OsDMC1 is severely reduced and in vitro OsBRCA2 directly interacts with OsRAD51 and OsDMC1
- OsDMC1~DMC1B~OsDMC1B, OsRad51~OsRad51A1~COLD11, Rice OsBRCA2 Is Required for DNA Double-Strand Break Repair in Meiotic Cells, These results indicate that OsBRCA2 is essential for facilitating the loading of OsRAD51 and OsDMC1 onto resected ends of programmed double-strand breaks (DSB) during meiosis to promote single-end invasions of homologous chromosomes and accurate recombination
- OsBRCA2, OsRad51~OsRad51A1~COLD11, Rice OsBRCA2 Is Required for DNA Double-Strand Break Repair in Meiotic Cells, In the absence of OsBRCA2, localization to the meiotic chromosome axes of the strand-invasion proteins OsRAD51 and OsDMC1 is severely reduced and in vitro OsBRCA2 directly interacts with OsRAD51 and OsDMC1
- OsBRCA2, OsRad51~OsRad51A1~COLD11, Rice OsBRCA2 Is Required for DNA Double-Strand Break Repair in Meiotic Cells, These results indicate that OsBRCA2 is essential for facilitating the loading of OsRAD51 and OsDMC1 onto resected ends of programmed double-strand breaks (DSB) during meiosis to promote single-end invasions of homologous chromosomes and accurate recombination
- DMC1A~DMC1~OsDMC1A, OsRad51~OsRad51A1~COLD11, OsRAD51 Plays a Vital Role in Promoting Homologous Recombination in Rice Meiosis., Moreover, the Osrad51 Osdmc1 (Osrad51a1 Osrad51a2 Osdmc1a Osdmc1b) quadruple mutant PMCs exhibited similar defective phenotypes as Osrad51 in homologous pairing, synapsis, and DSB repair
- OsBRCA2, OsRad51~OsRad51A1~COLD11, OsRAD51 Plays a Vital Role in Promoting Homologous Recombination in Rice Meiosis., In addition, both OsRAD51 and OsDMC1 can interact with OsFIGL1 and OsBRCA2, two important components in rice meiosis
- OsDMC1~DMC1B~OsDMC1B, OsRad51~OsRad51A1~COLD11, OsRAD51 Plays a Vital Role in Promoting Homologous Recombination in Rice Meiosis., In the homologous recombination pathway, the detailed mechanisms of how OsRAD51 and OsDMC1 work in rice meiosis remain to be explored
- OsDMC1~DMC1B~OsDMC1B, OsRad51~OsRad51A1~COLD11, OsRAD51 Plays a Vital Role in Promoting Homologous Recombination in Rice Meiosis., Most OsRAD51 foci can co-localize with OsDMC1 signals
- OsDMC1~DMC1B~OsDMC1B, OsRad51~OsRad51A1~COLD11, OsRAD51 Plays a Vital Role in Promoting Homologous Recombination in Rice Meiosis., OsRAD51 is essential for the loading of OsDMC1 onto chromosomes, and vice versa
- OsDMC1~DMC1B~OsDMC1B, OsRad51~OsRad51A1~COLD11, OsRAD51 Plays a Vital Role in Promoting Homologous Recombination in Rice Meiosis., In addition, both OsRAD51 and OsDMC1 can interact with OsFIGL1 and OsBRCA2, two important components in rice meiosis
- OsDMC1~DMC1B~OsDMC1B, OsRad51~OsRad51A1~COLD11, OsRAD51 Plays a Vital Role in Promoting Homologous Recombination in Rice Meiosis., Moreover, the Osrad51 Osdmc1 (Osrad51a1 Osrad51a2 Osdmc1a Osdmc1b) quadruple mutant PMCs exhibited similar defective phenotypes as Osrad51 in homologous pairing, synapsis, and DSB repair
- OsRad51~OsRad51A1~COLD11, OsRad51A2, OsRAD51 Plays a Vital Role in Promoting Homologous Recombination in Rice Meiosis., Osrad51 (Osrad51a1 Osrad51a2) mutant plants show normal vegetative growth but exhibit complete sterility, indicating that OsRAD51A1 and OsRAD51A2 are functionally redundant in rice fertility
- OsRad51~OsRad51A1~COLD11, OsRad51A2, OsRAD51 Plays a Vital Role in Promoting Homologous Recombination in Rice Meiosis., Moreover, the Osrad51 Osdmc1 (Osrad51a1 Osrad51a2 Osdmc1a Osdmc1b) quadruple mutant PMCs exhibited similar defective phenotypes as Osrad51 in homologous pairing, synapsis, and DSB repair
- OsRad51~OsRad51A1~COLD11, Sc, OsRAD51 Plays a Vital Role in Promoting Homologous Recombination in Rice Meiosis., In contrast to the wild type, Osrad51 chromosomes are not paired perfectly at pachytene and synaptonemal complex (SC) formation is deficient
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