TCD5,CSV1,TSV

• Symbol: TCD5,CSV1,TSV
• MSU: LOC_Os05g34040
• RAPdb: Os05g0411200

• Temperature-sensitive albino gene TCD5, encoding a monooxygenase, affects chloroplast development at low temperatures., 2016, J Exp Bot.
• Heavy-ion beam mutagenesis identified an essential gene for chloroplast development under cold stress conditions during both early growth and tillering stages in rice., 2016, Biosci Biotechnol Biochem.
• TSV, a putative plastidic oxidoreductase, protects rice chloroplasts from cold stress during development by interacting with plastidic thioredoxin Z., 2017, New Phytol.

Genbank accession number

• The development of chloroplasts and etioplasts is impaired in tcd5 plants at 20 °C, and the temperature-sensitive period for the albino phenotype is the P4 stage of leaf development
• Although the phenotype and temperature dependence of the TCD5 orthologous mutant phenotype were different in rice and Arabidopsis, OsTCD5 could rescue the phenotype of the Arabidopsis mutant, suggesting that TCD5 function is conserved between monocots and dicots
• The development of thylakoid membranes is arrested at the mid-P4 stage in tcd5 plants at 20 °C
• The transcription of some genes involved in plastid transcription/translation and photosynthesis varied in the tcd5 mutant
• Furthermore, CSV1 transcripts were more abundant in immature than in mature leaves, and they did not markedly increase or decrease with temperature
• The growth of csv1 plants displayed sensitivity to low temperatures
• Taken together, our results indicate that CSV1 supports chloroplast development under cold stress conditions, in both the early growth and tillering stages in rice
• These results provide novel insights into ways in which rice chloroplast development and chlorophyll synthesis are protected by TSV under cold stress
• We isolated and identified TSV by map-based cloning and rescue experiments, combined with genetic, cytological and molecular biological analyses
• The activity of plastid-encoded RNA polymerase (PEP) and the expression of genes participating in chlorophyll synthesis were severely reduced in the tsv mutant under cold stress, but not at normal temperatures
• We demonstrated that TSV protects rice chloroplasts from cold stress by interacting with OsTrxZ

• OsTRXZ~WP2, TCD5~CSV1~TSV, TSV, a putative plastidic oxidoreductase, protects rice chloroplasts from cold stress during development by interacting with plastidic thioredoxin Z, Strikingly, TSV interacted with OsTrxZ (a subunit of PEP in chloroplasts) and enhanced OsTrxZ stability under low temperatures.