OsEMF2b

• Symbol: OsEMF2b
• MSU: LOC_Os09g13630
• RAPdb: Os09g0306800

• Expression, imprinting, and evolution of rice homologs of the polycomb group genes, 2009, Mol Plant.
• OsVIL2 functions with PRC2 to induce flowering by repressing OsLFL1 in rice, 2013, Plant J.
• The polycomb group gene EMF2B is essential for maintenance of floral meristem determinacy in rice, 2014, Plant J.
• Down-Regulation of OsEMF2b Caused Semi-sterility Due to Anther and Pollen Development Defects in Rice., 2017, Front Plant Sci.
• OsABF1 Represses Gibberellin Biosynthesis to Regulate Plant Height and Seed Germination in Rice Oryza sativa L. , 2021, Int J Mol Sci.

Genbank accession number

• One line with a T-DNA insertion in OsEMF2b displays pleiotropic phenotypes including altered flowering time and abnormal flower organs, suggesting important roles in rice development for this gene
• As observed from osvil2, a null mutation of OsEMF2b caused late flowering by increasing OsLFL1 expression and decreasing Ehd1 expression
• The polycomb group gene EMF2B is essential for maintenance of floral meristem determinacy in rice
• Loss of EMF2B in rice results in complete sterility, and mutant flowers have severe floral organ defects and indeterminacy that resemble loss-of-function mutants in E-function floral organ specification genes.
• Down-Regulation of OsEMF2b Caused Semi-sterility Due to Anther and Pollen Development Defects in Rice.
• Overall, our work suggests that OsEMF2b plays an essential role during post-meiotic anther and pollen development
• Its biological function in floral organ, flowering time and meristem determinacy have been well-studied, but its role, if only, on male reproduction is still unknown, because null mutants of OsEMF2b barely have anthers
• OsEMF2b was highly expressed in tapetum and microspores, and the protein was localized in the nucleus

• OsEMF2b, OsFIE1~Epi-df, Expression, imprinting, and evolution of rice homologs of the polycomb group genes, The rice genome contains two E(z)-like genes, OsiEZ1 and OsCLF, two homologs of Su(z)12, OsEMF2a and OsEMF2b, and two ESC-like genes, OsFIE1 and OsFIE2
• OsCLF~SDG711, OsEMF2b, Expression, imprinting, and evolution of rice homologs of the polycomb group genes, The rice genome contains two E(z)-like genes, OsiEZ1 and OsCLF, two homologs of Su(z)12, OsEMF2a and OsEMF2b, and two ESC-like genes, OsFIE1 and OsFIE2
• OsEMF2b, OsFIE2, Expression, imprinting, and evolution of rice homologs of the polycomb group genes, The rice genome contains two E(z)-like genes, OsiEZ1 and OsCLF, two homologs of Su(z)12, OsEMF2a and OsEMF2b, and two ESC-like genes, OsFIE1 and OsFIE2
• OsEMF2b, OsiEZ1~OsSET1~SDG718, Expression, imprinting, and evolution of rice homologs of the polycomb group genes, The rice genome contains two E(z)-like genes, OsiEZ1 and OsCLF, two homologs of Su(z)12, OsEMF2a and OsEMF2b, and two ESC-like genes, OsFIE1 and OsFIE2
• OsEMF2a, OsEMF2b, Expression, imprinting, and evolution of rice homologs of the polycomb group genes, The rice genome contains two E(z)-like genes, OsiEZ1 and OsCLF, two homologs of Su(z)12, OsEMF2a and OsEMF2b, and two ESC-like genes, OsFIE1 and OsFIE2
• Ehd1, OsEMF2b, OsVIL2 functions with PRC2 to induce flowering by repressing OsLFL1 in rice, As observed from osvil2, a null mutation of OsEMF2b caused late flowering by increasing OsLFL1 expression and decreasing Ehd1 expression
• OsEMF2b, OsLFL1, OsVIL2 functions with PRC2 to induce flowering by repressing OsLFL1 in rice, As observed from osvil2, a null mutation of OsEMF2b caused late flowering by increasing OsLFL1 expression and decreasing Ehd1 expression
• OsEMF2b, OsVIL2, OsVIL2 functions with PRC2 to induce flowering by repressing OsLFL1 in rice, We showed that OsVIL2 physically interacts with OsEMF2b, a component of polycomb repression complex 2
• OsEMF2b, OsVIL2, OsVIL2 functions with PRC2 to induce flowering by repressing OsLFL1 in rice, As observed from osvil2, a null mutation of OsEMF2b caused late flowering by increasing OsLFL1 expression and decreasing Ehd1 expression
• OsEMF2b, OsLG3b~OsMADS1~LHS1~AFO, The polycomb group gene EMF2B is essential for maintenance of floral meristem determinacy in rice, Transcriptome analysis identified the E-function genes OsMADS1, OsMADS6 and OsMADS34 as differentially expressed in the emf2b mutant compared with wild type.
• OsEMF2b, OsMADS6~MFO1~AFG1, The polycomb group gene EMF2B is essential for maintenance of floral meristem determinacy in rice, Transcriptome analysis identified the E-function genes OsMADS1, OsMADS6 and OsMADS34 as differentially expressed in the emf2b mutant compared with wild type.
• OsEMF2b, OsMADS34~PAP2, The polycomb group gene EMF2B is essential for maintenance of floral meristem determinacy in rice, Transcriptome analysis identified the E-function genes OsMADS1, OsMADS6 and OsMADS34 as differentially expressed in the emf2b mutant compared with wild type.
• OsEMF2b, OsMADS34~PAP2, The polycomb group gene EMF2B is essential for maintenance of floral meristem determinacy in rice, OsMADS1 and OsMADS6, known to be required for meristem determinacy in rice, have reduced expression in the emf2b mutant, whereas OsMADS34 which interacts genetically with OsMADS1 was ectopically expressed.
• OsEMF2b, OsLG3b~OsMADS1~LHS1~AFO, The polycomb group gene EMF2B is essential for maintenance of floral meristem determinacy in rice, OsMADS1 and OsMADS6, known to be required for meristem determinacy in rice, have reduced expression in the emf2b mutant, whereas OsMADS34 which interacts genetically with OsMADS1 was ectopically expressed.
• OsEMF2b, OsMADS6~MFO1~AFG1, The polycomb group gene EMF2B is essential for maintenance of floral meristem determinacy in rice, OsMADS1 and OsMADS6, known to be required for meristem determinacy in rice, have reduced expression in the emf2b mutant, whereas OsMADS34 which interacts genetically with OsMADS1 was ectopically expressed.
• OsEMF2b, OSVP1~VP1, Rice PcG gene OsEMF2b controls seed dormancy and seedling growth by regulating the expression of OsVP1., Rice PcG gene OsEMF2b controls seed dormancy and seedling growth by regulating the expression of OsVP1.
• OsEMF2b, OSVP1~VP1, Rice PcG gene OsEMF2b controls seed dormancy and seedling growth by regulating the expression of OsVP1., Downregulation of OsEMF2b led to vivipary and decreased expression level of OsVP1, which was involved in ABA signal pathway in seed dormancy
• OsEMF2b, OSVP1~VP1, Rice PcG gene OsEMF2b controls seed dormancy and seedling growth by regulating the expression of OsVP1., While, the seedlings with downregulation of OsEMF2b exhibited hyper-sensitive response to ABA and the expression of OsVP1 is upregulated
• OsEMF2b, OSVP1~VP1, Rice PcG gene OsEMF2b controls seed dormancy and seedling growth by regulating the expression of OsVP1., Yeast one-hybrid assay and ChIP analyses proved that OsEMF2b could bind to the promoter of OsVP1 directly and affected H3K27me3 enrichments of OsVP1 in seedling
• OsEMF2b, OSVP1~VP1, Rice PcG gene OsEMF2b controls seed dormancy and seedling growth by regulating the expression of OsVP1., Interestingly, both H3K27me3 and H3K4me3 enrichments of OsVP1 were changed with OsEMF2b mis-expression in seed and seedling
• OsEMF2b, OSVP1~VP1, Rice PcG gene OsEMF2b controls seed dormancy and seedling growth by regulating the expression of OsVP1., We proposed that OsEMF2b may play a pivotal role in seed dormancy and seedling growth by regulating the expression of OsVP1 indirectly or directly
• OsCLF~SDG711, OsEMF2b, Regulation of plant height in rice by the Polycomb group genes OsEMF2b, OsFIE2 and OsCLF., Regulation of plant height in rice by the Polycomb group genes OsEMF2b, OsFIE2 and OsCLF.
• OsCLF~SDG711, OsEMF2b, Regulation of plant height in rice by the Polycomb group genes OsEMF2b, OsFIE2 and OsCLF., To determine how PcG genes regulate plant height, we investigated the phenotypes of the emf2b mutant and OsEMF2b, OsFIE2 and OsCLF RNAi transgenic plants; they all exhibited dwarf phenotype
• OsCLF~SDG711, OsEMF2b, Regulation of plant height in rice by the Polycomb group genes OsEMF2b, OsFIE2 and OsCLF., Reduced cell expansion and cell division of stem caused significant reduction of stem length during mature period of OsEMF2b, OsFIE2 and OsCLF RNAi transgenic plants
• OsCLF~SDG711, OsEMF2b, Regulation of plant height in rice by the Polycomb group genes OsEMF2b, OsFIE2 and OsCLF., H3K27me3-modified sites were observed in 95 of the 109 up-regulated genes, and some of them were up-regulated in OsFIE2, OsCLF and OsEMF2b RNAi transgenic plants, and their H3K27me3 levels were reduced in emf2b
• OsCLF~SDG711, OsEMF2b, Regulation of plant height in rice by the Polycomb group genes OsEMF2b, OsFIE2 and OsCLF., Moreover, OsEMF2b interacted with OsCLF
• OsCLF~SDG711, OsEMF2b, Regulation of plant height in rice by the Polycomb group genes OsEMF2b, OsFIE2 and OsCLF., Therefore, we speculated that these PcG genes, OsFIE2, OsCLF and OsEMF2b, may work as a PRC2 to regulate rice height
• D6~OSH15~Oskn3~RLB, OsEMF2b, RLB RICE LATERAL BRANCH recruits PRC2-mediated H3K27 tri-methylation on OsCKX4 to regulate lateral branching., Meanwhile, RLB physically binds to Polycomb repressive complex 2 (PRC2) component OsEMF2b and co-localized with H3K27me3, a suppressing histone modification mediated by PRC2, in the OsCKX4 promoter
• OsABF1~OsABI5~OREB1~OsbZIP10, OsEMF2b, OsABF1 Represses Gibberellin Biosynthesis to Regulate Plant Height and Seed Germination in Rice Oryza sativa L.., Moreover, OsABF1 interacts with and transcriptionally antagonizes to the polycomb repression complex component OsEMF2b, whose mutant showed as similar but more severe phenotype to OsABF1 overexpression lines
• OsABF1~OsABI5~OREB1~OsbZIP10, OsEMF2b, OsABF1 Represses Gibberellin Biosynthesis to Regulate Plant Height and Seed Germination in Rice Oryza sativa L. , Moreover, OsABF1 interacts with and transcriptionally antagonizes to the polycomb repression complex component OsEMF2b, whose mutant showed as similar but more severe phenotype to OsABF1 overexpression lines
• OsEMF2b, OsMADS8~OsMADS24, A Novel Embryo Phenotype Associated With Interspecific Hybrid Weakness in Rice Is Controlled by the MADS-Domain Transcription Factor OsMADS8., Overexpression of OsMADS8 led to decreased expression of OsEMF2b and ABA signaling-related genes including OsVP1/ABI3
• OsEMF2b, OsMADS8~OsMADS24, A Novel Embryo Phenotype Associated With Interspecific Hybrid Weakness in Rice Is Controlled by the MADS-Domain Transcription Factor OsMADS8., Our results suggest that OsMADS8 is critical for floral organ determination and seed germination and that these effects are the result of regulation of the expression of OsEMF2b and its role in ABA signaling and catabolism
• OsEMF2b, PACP, A coiled-coil protein associates Polycomb Repressive Complex 2 with KNOX/BELL transcription factors to maintain silencing of cell differentiation-promoting genes in the shoot apex, A loss-of-function mutation of PACP resulted in differential gene expression similar to that observed in PRC2 gene knockdown plants, reduced H3K27me3 levels, and reduced genome-wide binding of the PRC2 core component EMF2b.